##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546581_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1328796
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.329343255097097	31.0	31.0	33.0	30.0	34.0
2	31.682678153757237	31.0	31.0	34.0	30.0	34.0
3	31.789110593349168	31.0	31.0	34.0	30.0	34.0
4	35.54568571850005	37.0	35.0	37.0	33.0	37.0
5	35.457821215596674	37.0	35.0	37.0	33.0	37.0
6	35.511547295446405	37.0	35.0	37.0	33.0	37.0
7	35.88124512716775	37.0	35.0	37.0	35.0	37.0
8	34.54384570694072	37.0	35.0	37.0	32.0	37.0
9	36.805751221406446	39.0	37.0	39.0	32.0	39.0
10	36.735931625320966	39.0	37.0	39.0	32.0	39.0
11	36.66644165093814	39.0	35.0	39.0	32.0	39.0
12	36.22782353348445	38.0	35.0	39.0	32.0	39.0
13	36.00710869087505	38.0	35.0	39.0	31.0	39.0
14	37.03370946330362	39.0	35.0	41.0	31.0	41.0
15	37.251091965960164	39.0	35.0	41.0	32.0	41.0
16	37.37077474646221	39.0	35.0	41.0	32.0	41.0
17	37.237318595179396	39.0	35.0	41.0	32.0	41.0
18	37.18673596248032	39.0	35.0	41.0	32.0	41.0
19	37.144522560272605	39.0	35.0	41.0	32.0	41.0
20	36.92217466036924	39.0	35.0	41.0	31.0	41.0
21	36.84689974984874	38.0	35.0	41.0	31.0	41.0
22	36.749882600489464	38.0	35.0	41.0	31.0	41.0
23	36.65868425251129	38.0	35.0	41.0	31.0	41.0
24	36.544547093760066	38.0	35.0	41.0	31.0	41.0
25	36.49582027640059	38.0	35.0	40.0	31.0	41.0
26	36.3380037266819	38.0	35.0	40.0	31.0	41.0
27	36.194156213594866	38.0	35.0	40.0	30.0	41.0
28	36.237907097854	38.0	35.0	40.0	30.0	41.0
29	36.20928720435643	38.0	35.0	40.0	30.0	41.0
30	36.17856390296178	38.0	35.0	40.0	30.0	41.0
31	36.07693280232632	38.0	35.0	40.0	30.0	41.0
32	35.912480170018576	38.0	35.0	40.0	30.0	41.0
33	35.772452656389696	38.0	35.0	40.0	29.0	41.0
34	35.62636552187093	38.0	35.0	40.0	28.0	41.0
35	35.4875827440781	38.0	35.0	40.0	27.0	41.0
36	35.35672894861213	38.0	35.0	40.0	27.0	41.0
37	35.29955087161611	38.0	34.0	40.0	27.0	41.0
38	35.210186514709555	38.0	34.0	40.0	26.0	41.0
39	35.135059106138186	38.0	34.0	40.0	26.0	41.0
40	35.01128389910867	37.0	34.0	40.0	25.0	41.0
41	34.93282941851119	37.0	34.0	40.0	25.0	41.0
42	34.87997706194179	37.0	34.0	40.0	25.0	41.0
43	34.775410220982	37.0	34.0	40.0	24.0	41.0
44	34.69193766387015	37.0	34.0	40.0	24.0	41.0
45	34.61535706007544	37.0	34.0	40.0	24.0	41.0
46	34.55303522888389	37.0	34.0	40.0	24.0	41.0
47	34.45600679111015	36.0	34.0	40.0	24.0	41.0
48	34.4113197210106	36.0	34.0	40.0	23.0	41.0
49	34.37948789731456	36.0	34.0	40.0	24.0	41.0
50	34.238152432728576	36.0	34.0	40.0	24.0	41.0
51	33.885078672723274	35.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	5.0
11	4.0
12	4.0
13	6.0
14	13.0
15	53.0
16	117.0
17	332.0
18	725.0
19	1445.0
20	2491.0
21	4276.0
22	6460.0
23	9326.0
24	12135.0
25	16498.0
26	20790.0
27	23452.0
28	25136.0
29	27967.0
30	33387.0
31	40153.0
32	50179.0
33	64544.0
34	106010.0
35	162966.0
36	100915.0
37	125925.0
38	178650.0
39	314524.0
40	306.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.27298697467482	23.499318179765744	26.25113260425227	13.97656224130717
2	31.818653879150748	26.73781378029434	27.59370136574764	13.84983097480727
3	29.04682133299619	26.541320112342298	30.52078723897423	13.891071315687284
4	25.52491127306223	26.920610838684038	31.953362291879266	15.601115596374463
5	24.32367346078706	31.005436500410898	29.02304040650333	15.647849632298714
6	20.276626359501385	41.27405561124507	27.354612747178646	11.094705282074901
7	82.21690914180958	4.759797591202863	10.617054837612395	2.4062384293751635
8	78.8555203357024	8.251755724731261	8.503186343125657	4.389537596440688
9	75.85287734159344	5.744674126050951	11.927564502000306	6.474884030355299
10	43.673520991935554	23.00353101604761	18.079449366193156	15.243498625823678
11	37.2309970830737	23.618147556133522	24.10385040292114	15.047004957871637
12	32.23180984891586	21.19384766359923	27.747524826986236	18.826817660498676
13	23.54876143516386	30.734890833506423	30.099202586401525	15.617145144928191
14	18.317710167700685	35.21518728232174	29.96133341762016	16.50576913235741
15	16.334486256731658	24.050945367084186	42.69172995704382	16.922838419140334
16	18.79438228290874	21.36979641720776	39.13655670245846	20.69926459742504
17	19.391539408607493	22.33405278161584	29.878100174895167	28.396307634881502
18	21.37205410010265	23.595420214991616	33.547662696155015	21.48486298875072
19	26.031836339061826	26.037932082878037	26.8238314985897	21.10640007947044
20	27.6859653400522	24.986002366051675	26.162179898193553	21.165852395702576
21	22.780772970418333	28.730670471614907	29.456741290611955	19.03181526735481
22	22.629432960364117	23.631618397406374	28.3382099283863	25.400738713843207
23	20.757663328306226	28.87764562807233	26.778075791919903	23.58661525170154
24	20.462283149557944	25.7977146228616	33.43206933193658	20.307932895643876
25	19.813425085566184	26.458764174485776	30.86395503899771	22.863855700950335
26	17.74659165138968	29.57293670360236	29.393074632976017	23.287397012031942
27	20.97440088621579	28.23676471030918	29.204106574673617	21.584727828801412
28	17.919454905041857	27.144497725760765	34.40716257424014	20.52888479495724
29	19.513228516642133	24.38470615504562	33.5958265979127	22.50623873039955
30	20.84571296120699	27.156538701200184	30.975936110584318	21.02181222700851
31	24.73600161349071	24.9127781841607	27.39111195397939	22.960108248369202
32	26.357469468601654	25.84316930514541	27.658346352638024	20.141014873614914
33	24.759556771694076	24.756697040027213	28.301710721585554	22.182035466693158
34	19.088784132402566	25.68219651473966	30.442219874232013	24.786799478625763
35	19.5866032107261	25.050120560266585	31.787798879587235	23.575477349420076
36	25.130343559131724	25.15344718075611	28.54983007173411	21.166379188378052
37	19.425254139837868	27.763027582864485	30.80766347881842	22.004054798479224
38	20.520982904825118	29.446957998067425	26.14125870336756	23.890800393739898
39	20.13108106887739	26.69815381744075	29.02221258944187	24.14855252423999
40	22.025277017691202	24.24969671793112	28.324061782244982	25.4009644821327
41	18.636419736362843	23.32630441392057	29.213889867218146	28.82338598249844
42	22.10407015072291	24.614989810324534	28.001890433143988	25.279049605808567
43	21.672325925123197	24.649682870809364	29.476608900086998	24.201382303980445
44	21.257740089524653	26.88253125385687	29.33437487770884	22.52535377890963
45	18.953323158709086	28.396232378785008	27.980818726125	24.669625736380905
46	22.883798566521875	27.669333742726497	28.09362761477307	21.353240075978555
47	21.20438351710872	25.63598927149088	29.922426015731535	23.23720119566886
48	22.276030331217132	24.18031059696146	29.807284188092076	23.73637488372933
49	21.56937558511615	22.32005514766751	31.98421729144278	24.12635197577356
50	20.509393465964678	26.57909867278348	29.463740107586116	23.447767753665723
51	20.063425838127145	28.040195786260647	26.91752533872769	24.97885303688452
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1649.0
1	2061.0
2	2473.0
3	7717.5
4	12962.0
5	9156.5
6	5351.0
7	5699.0
8	6047.0
9	6458.5
10	6870.0
11	7050.5
12	7231.0
13	7208.0
14	7185.0
15	7016.0
16	6847.0
17	6594.0
18	6341.0
19	6278.5
20	6216.0
21	6438.0
22	6660.0
23	6974.0
24	7288.0
25	8080.5
26	9447.5
27	10022.0
28	11534.0
29	13046.0
30	14711.5
31	16377.0
32	20596.0
33	24815.0
34	26863.0
35	28911.0
36	31298.0
37	33685.0
38	40468.0
39	47251.0
40	60808.0
41	74365.0
42	89095.5
43	103826.0
44	121055.5
45	138285.0
46	135711.0
47	133137.0
48	122956.0
49	112775.0
50	104544.0
51	96313.0
52	88140.0
53	79967.0
54	73610.5
55	67254.0
56	61369.5
57	55485.0
58	51915.5
59	48346.0
60	44436.5
61	40527.0
62	36353.5
63	32180.0
64	29339.0
65	26498.0
66	22376.0
67	18254.0
68	15340.0
69	12426.0
70	10757.0
71	9088.0
72	7592.0
73	6096.0
74	4832.5
75	2776.5
76	1984.0
77	1551.5
78	1119.0
79	938.5
80	758.0
81	510.0
82	262.0
83	174.5
84	87.0
85	53.5
86	20.0
87	23.5
88	27.0
89	22.5
90	18.0
91	18.0
92	18.0
93	14.0
94	10.0
95	6.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1328796.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.299251091014664
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.31496954828407	21.92538520065803
2	6.662207054655285	3.637465264107277
3	2.3761497759720127	1.9460132809235464
4	1.2897545452296575	1.408373327040074
5	0.8640377403020703	1.179379161230957
6	0.6616298009956757	1.083719884000741
7	0.5042357624566058	0.9635681081860475
8	0.40149666093862924	0.8768446527334094
9	0.3565907805105066	0.8761195128507495
>10	5.152951563669192	35.70629055496822
>50	1.322241388257652	23.79540730039495
>100	0.0869182602759354	3.5881285054345997
>500	0.003692605828525903	0.6631442737101224
>1k	0.0028404660219430024	1.7639389544384783
>5k	2.840466021943002E-4	0.5862220193227929
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7560	0.5689360895126114	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCG	4724	0.35550979984888575	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTC	4687	0.35272532427851977	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGC	2636	0.19837507036445023	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2344	0.17640029018750808	No Hit
GCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGC	1837	0.13824544926384486	No Hit
GAACTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCT	1509	0.11356144961303316	No Hit
CTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGCT	1498	0.11273363255157301	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.5051219299275434E-4	0.0	0.0	0.11965719342923971	0.0
2	1.5051219299275434E-4	0.0	0.0	0.4276051402924151	0.0
3	1.5051219299275434E-4	0.0	0.0	0.6256039301743834	0.0
4	1.5051219299275434E-4	0.0	0.0	1.1091243501636068	0.0
5	1.5051219299275434E-4	0.0	0.0	2.05546976360555	0.0
6	1.5051219299275434E-4	0.0	0.0	2.980969238317996	0.0
7	1.5051219299275434E-4	0.0	0.0	3.5583340106382018	0.0
8	1.5051219299275434E-4	0.0	0.0	5.02928967275639	0.0
9	1.5051219299275434E-4	0.0	0.0	5.669568541747567	0.0
10	1.5051219299275434E-4	0.0	0.0	7.0244793030683415	0.0
11	1.5051219299275434E-4	0.0	0.0	7.717362183510486	0.0
12	1.5051219299275434E-4	0.0	0.0	8.577313598174587	0.0
13	1.5051219299275434E-4	0.0	0.0	8.8678773867471	0.0
14	1.5051219299275434E-4	0.0	0.0	8.990770592325685	0.0
15	1.5051219299275434E-4	0.002257682894891315	0.0	9.191779626067508	0.0
16	1.5051219299275434E-4	0.002257682894891315	0.0	9.510112914247182	0.0
17	1.5051219299275434E-4	0.002257682894891315	0.0	9.812266141680137	0.0
18	1.5051219299275434E-4	0.002257682894891315	0.0	10.299850390880165	0.0
19	1.5051219299275434E-4	0.002257682894891315	0.0	10.552635619011497	0.0
20	1.5051219299275434E-4	0.002257682894891315	0.0	10.770125737886026	0.0
21	1.5051219299275434E-4	0.002257682894891315	0.0	11.02118007579794	0.0
22	1.5051219299275434E-4	0.002257682894891315	0.0	11.278856950201536	0.0
23	1.5051219299275434E-4	0.002257682894891315	0.0	11.553616958509808	0.0
24	1.5051219299275434E-4	0.002257682894891315	0.0	11.772386431024778	0.0
25	1.5051219299275434E-4	0.002257682894891315	0.0	11.95074337972119	0.0
26	1.5051219299275434E-4	0.002257682894891315	0.0	12.125488035785779	0.0
27	1.5051219299275434E-4	0.002257682894891315	0.0	12.315660191632125	0.0
28	1.5051219299275434E-4	0.002257682894891315	0.0	12.497177896381386	0.0
29	1.5051219299275434E-4	0.002257682894891315	0.0	12.69653129600029	0.0
30	1.5051219299275434E-4	0.002257682894891315	0.0	12.95428342650038	0.0
31	1.5051219299275434E-4	0.002257682894891315	0.0	13.179524923314037	0.0
32	1.5051219299275434E-4	0.002257682894891315	0.0	13.385651371617614	0.0
33	1.5051219299275434E-4	0.002257682894891315	0.0	13.591476795535206	0.0
34	1.5051219299275434E-4	0.002257682894891315	0.0	13.799635158444186	0.0
35	1.5051219299275434E-4	0.002257682894891315	0.0	14.031047655170545	0.0
36	1.5051219299275434E-4	0.002257682894891315	0.0	14.236572054702151	0.0
37	1.5051219299275434E-4	0.002257682894891315	0.0	14.449998344365877	0.0
38	1.5051219299275434E-4	0.002257682894891315	0.0	14.682765450829171	0.0
39	1.5051219299275434E-4	0.002257682894891315	0.0	14.997335934184028	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTAGCG	85	0.0	45.000004	1
CGCACAT	25	3.8911356E-5	45.0	28
TAAATCG	20	7.0336845E-4	45.0	35
TTCGACT	20	7.0336845E-4	45.0	27
CGGTCTA	210	0.0	43.92857	31
CGTAAGG	230	0.0	42.06522	2
CGTTTTT	5045	0.0	41.43211	1
GCGTAAG	120	0.0	41.250004	1
CACGACG	225	0.0	40.0	26
ATAGGGT	405	0.0	39.444447	4
ATAGACG	40	3.458772E-7	39.375	1
TCACGAC	245	0.0	38.57143	25
CTCACGA	245	0.0	38.57143	24
TAATCGG	35	6.249027E-6	38.571426	2
TACGGGT	100	0.0	38.25	4
TAAGGGA	915	0.0	38.11475	4
GCGCGAC	480	0.0	37.968754	9
GATGAAT	1055	0.0	37.962086	20
CGATGAA	1030	0.0	37.79126	19
ACCCGCT	460	0.0	37.663044	34
>>END_MODULE