##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546580_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1642884
Sequences flagged as poor quality	0
Sequence length	51
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.312024464295714	31.0	31.0	33.0	30.0	34.0
2	31.652002210746467	31.0	31.0	34.0	30.0	34.0
3	31.50075050946993	31.0	31.0	34.0	30.0	34.0
4	35.43692555286922	37.0	35.0	37.0	33.0	37.0
5	35.41194448299454	37.0	35.0	37.0	33.0	37.0
6	35.4815641274734	37.0	35.0	37.0	33.0	37.0
7	35.955823417843256	37.0	35.0	37.0	35.0	37.0
8	34.655905712150094	37.0	35.0	37.0	33.0	37.0
9	36.91327202650948	39.0	37.0	39.0	33.0	39.0
10	36.80913442458506	39.0	37.0	39.0	32.0	39.0
11	36.729438597003806	39.0	37.0	39.0	32.0	39.0
12	36.62876746014935	39.0	35.0	39.0	32.0	39.0
13	36.61497342478227	39.0	35.0	39.0	32.0	39.0
14	37.7932574667475	40.0	36.0	41.0	33.0	41.0
15	37.916990487459856	40.0	36.0	41.0	33.0	41.0
16	37.92309986584567	40.0	36.0	41.0	33.0	41.0
17	37.82968547992433	40.0	36.0	41.0	33.0	41.0
18	37.567565938922044	39.0	36.0	41.0	32.0	41.0
19	37.297029492039606	38.0	36.0	41.0	32.0	41.0
20	36.95067150206588	38.0	35.0	41.0	32.0	41.0
21	36.86181373730586	38.0	35.0	41.0	32.0	41.0
22	36.77612905110769	38.0	35.0	41.0	32.0	41.0
23	36.68659990601893	38.0	35.0	40.0	32.0	41.0
24	36.50527000080346	38.0	35.0	40.0	31.0	41.0
25	36.385788649715984	38.0	35.0	40.0	31.0	41.0
26	36.28682305019709	38.0	35.0	40.0	31.0	41.0
27	36.15207099223073	37.0	35.0	40.0	31.0	41.0
28	36.07312993491932	37.0	35.0	40.0	31.0	41.0
29	35.953442239379044	37.0	35.0	40.0	30.0	41.0
30	35.8109117868334	37.0	35.0	40.0	30.0	41.0
31	35.55614760384787	37.0	35.0	40.0	29.0	41.0
32	35.10050800908646	37.0	34.0	40.0	25.0	41.0
33	34.57079805999693	37.0	34.0	40.0	22.0	41.0
34	34.05958728674697	37.0	33.0	40.0	18.0	41.0
35	33.667185875570034	37.0	33.0	40.0	16.0	41.0
36	33.44955760723216	37.0	33.0	40.0	15.0	41.0
37	33.336684148120014	37.0	33.0	40.0	15.0	41.0
38	33.19526393829388	37.0	33.0	40.0	13.0	41.0
39	33.084591486678306	37.0	33.0	40.0	12.0	41.0
40	32.93370438813696	36.0	32.0	40.0	10.0	41.0
41	32.831326496575535	36.0	32.0	40.0	10.0	41.0
42	32.71248000467471	36.0	32.0	40.0	10.0	41.0
43	32.61416813359921	36.0	32.0	40.0	10.0	41.0
44	32.48420277998934	36.0	31.0	40.0	10.0	41.0
45	32.37474222160542	35.0	31.0	40.0	10.0	41.0
46	32.30000718249128	35.0	31.0	40.0	10.0	41.0
47	32.22909712432527	35.0	31.0	40.0	10.0	41.0
48	32.11487907849854	35.0	31.0	40.0	10.0	41.0
49	32.04069063914434	35.0	31.0	40.0	10.0	41.0
50	31.937760669651663	35.0	31.0	40.0	10.0	41.0
51	31.623698325627373	35.0	30.0	39.0	10.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	2.0
12	6.0
13	9.0
14	25.0
15	61.0
16	188.0
17	525.0
18	1226.0
19	2352.0
20	4174.0
21	6570.0
22	9802.0
23	14919.0
24	23510.0
25	37925.0
26	52996.0
27	55179.0
28	48518.0
29	44005.0
30	44885.0
31	50196.0
32	59218.0
33	72874.0
34	104047.0
35	159595.0
36	145672.0
37	158002.0
38	206976.0
39	339155.0
40	270.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.443030670455126	21.967588703767277	24.514755758775422	25.074624867002175
2	41.445165939895936	22.19578497325435	24.838576551965932	11.520472534883778
3	24.502703781886	22.043735285023168	41.731674299585364	11.72188663350547
4	21.32919914004884	24.48937356502346	41.28398596614247	12.897441328785234
5	18.791649319124172	28.334928089871227	40.1581608926741	12.715261698330496
6	16.972409494523045	36.274076562922275	36.90254454970649	9.850969392848187
7	74.16768317178814	3.1354617854942894	20.41951835917813	2.2773366835394344
8	71.56567353507613	5.70740234855291	19.4476907681857	3.2792333481852647
9	68.17456375495775	5.409815909096443	22.692959454228053	3.7226608817177596
10	31.786115148726264	28.25245117731988	27.717477314283904	12.243956359669946
11	24.040772203028332	23.91252212572525	37.20627871474797	14.840426956498451
12	22.362808329742087	21.068681659812867	39.831601013826905	16.73690899661814
13	19.275432714665186	25.1752406134578	40.86575802065149	14.683568651225528
14	15.99802542358438	29.66417592477619	38.76061852206242	15.577180129577012
15	14.318661573184716	25.54008682292846	45.421648759133326	14.719602844753494
16	16.36859327864901	24.04351128868502	43.60508715161874	15.98280828104723
17	16.264021075133726	25.022095290963943	38.32699082832385	20.386892805578483
18	17.80776975124233	26.0443220580394	39.52902335161825	16.61888483910002
19	19.583488548187212	28.286963656594132	35.332257177013105	16.797290618205547
20	20.605654446692522	26.1868762493274	34.62733826612226	18.580131037857818
21	19.105548535380464	28.346553986769607	36.38163132637484	16.16626615147509
22	18.333674197326168	25.60868570148592	35.674764621239234	20.382875479948677
23	16.398662352302416	29.401893256005902	34.924133414166796	19.27531097752489
24	16.988722271322867	27.362735287457905	38.89398155925799	16.754560881961233
25	17.498253072036736	28.012020325232946	36.73740811889336	17.75231848383696
26	16.26438628655462	29.86692913194115	35.55168837239878	18.31699620910545
27	17.1494761650853	28.553689730985266	36.025976270996615	18.27085783293282
28	16.517112589811575	27.936178086827795	38.536378709635	17.010330613725618
29	16.31758541686449	26.877186703382588	37.04309007817959	19.76213780157333
30	17.53775677406317	27.541932357975362	36.380109612121124	18.540201255840337
31	19.72324278524838	27.97239488606621	32.83195892101938	19.472403407666032
32	19.020028194321693	28.276433393958428	32.798542076007806	19.904996335712077
33	17.897794366492096	29.11118496497622	32.67589190715839	20.31512876137329
34	15.909583391158474	28.59416733013408	33.763248044292844	21.733001234414605
35	16.694361866084275	28.264503154209304	32.74722987137253	22.29390510833388
36	19.585010262440928	28.2041215326219	31.303792598868817	20.907075606068354
37	18.26178841598068	29.219531019840716	31.969025202022785	20.549655362155818
38	17.974793107730065	30.500997027179032	30.770401318656702	20.753808546434197
39	19.01144572593074	29.472318191667824	29.701123146856386	21.815112935545052
40	19.70845172270227	28.554359285256904	31.22101134346673	20.516177648574093
41	18.39210802466881	28.33955410120252	30.788601021131136	22.479736852997533
42	19.193260144964587	30.700098120135078	29.022377721129427	21.084264013770905
43	19.315971182384146	29.848425086615975	30.784705432641623	20.050898298358252
44	19.822823766011478	30.974737108645527	29.734661729008256	19.467777396334736
45	18.108582224916674	31.167142658885226	29.854877155051724	20.869397961146376
46	20.546672802218538	30.101212258443077	29.30675568086365	20.045359258474733
47	19.72141672814392	29.505187219548063	29.64658490800324	21.12681114430477
48	19.302701834091756	29.030473240959193	30.12154236087271	21.54528256407634
49	19.22375529860903	27.996498839845053	30.355155933103006	22.42458992844291
50	17.935898091405114	28.802276971472118	32.511668504897486	20.750156432225282
51	17.504644271902336	29.668680198967184	30.752140747612128	22.074534781518356
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2233.0
1	2554.0
2	2875.0
3	39583.0
4	76291.0
5	49081.5
6	21872.0
7	22396.0
8	22920.0
9	25552.5
10	28185.0
11	30594.0
12	33003.0
13	33814.5
14	34626.0
15	33852.5
16	33079.0
17	30947.5
18	28816.0
19	26932.5
20	25049.0
21	23349.5
22	21650.0
23	20560.0
24	19470.0
25	19253.0
26	18867.0
27	18698.0
28	19892.5
29	21087.0
30	22725.5
31	24364.0
32	27722.5
33	31081.0
34	34288.0
35	37495.0
36	41631.5
37	45768.0
38	49968.5
39	54169.0
40	62149.5
41	70130.0
42	77554.0
43	84978.0
44	92074.5
45	99171.0
46	106092.5
47	113014.0
48	128729.5
49	144445.0
50	142337.5
51	140230.0
52	125566.5
53	110903.0
54	93708.0
55	76513.0
56	66211.0
57	55909.0
58	49008.5
59	42108.0
60	37320.0
61	32532.0
62	27932.5
63	23333.0
64	20522.0
65	17711.0
66	14178.0
67	10645.0
68	8951.5
69	7258.0
70	6063.5
71	4869.0
72	3951.5
73	3034.0
74	2582.0
75	1583.0
76	1036.0
77	836.0
78	636.0
79	489.0
80	342.0
81	223.5
82	105.0
83	79.0
84	53.0
85	34.5
86	16.0
87	12.5
88	9.0
89	6.0
90	3.0
91	3.0
92	3.0
93	2.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1642884.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.928618918766
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.53382307440143	30.558898559340346
2	8.536495404464398	6.817009438133123
3	3.9754250063358048	4.761996903543458
4	2.254319093963517	3.6004739209666856
5	1.5160610224210362	3.026711141092216
6	1.0535727425161927	2.524062272347694
7	0.8069743695198389	2.255496045443838
8	0.6721254825200309	2.146963380570723
9	0.5079645662918254	1.8254091232532292
>10	3.9796192095126663	30.88533341455337
>50	0.13054769577220954	3.384617071835563
>100	0.02823632710612867	2.086186422649323
>500	0.0024960026709560175	0.6734655559605844
>1k	0.002184002337086516	1.7906758974061119
>5k	0.0	0.0
>10k+	1.560001669347511E-4	3.6627008529036518
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	58802	3.579193661877528	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTT	4836	0.29436040523859264	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCT	3844	0.233978783651189	No Hit
CGTTCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTC	3230	0.1966054815799533	No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2043	0.1243544888135742	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTC	1850	0.11260685477489586	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1718	0.10457220351528167	No Hit
CTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTGCT	1654	0.10067661502577176	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.0868570148592354E-5	0.0	0.0	0.11029384910924935	0.0
2	6.0868570148592354E-5	0.0	0.0	0.33946401571869955	0.0
3	6.0868570148592354E-5	0.0	0.0	0.5999814959546749	0.0
4	6.0868570148592354E-5	0.0	0.0	0.9029852381543676	0.0
5	6.0868570148592354E-5	0.0	0.0	1.6035824805646655	0.0
6	6.0868570148592354E-5	0.0	0.0	2.9700210118304153	0.0
7	6.0868570148592354E-5	0.0	0.0	3.9235271632081146	0.0
8	6.0868570148592354E-5	0.0	0.0	5.643977298458077	0.0
9	6.0868570148592354E-5	0.0	0.0	6.666995356945469	0.0
10	6.0868570148592354E-5	0.0	0.0	7.8947752854127256	0.0
11	6.0868570148592354E-5	0.0	0.0	8.569016436948683	0.0
12	6.0868570148592354E-5	0.0	0.0	9.212701566270047	0.0
13	6.0868570148592354E-5	0.0	0.0	9.584121581316758	0.0
14	6.0868570148592354E-5	0.0	0.0	9.770196800261004	0.0
15	6.0868570148592354E-5	0.0	0.0	9.929794191190613	0.0
16	1.2173714029718471E-4	0.0	0.0	10.196581134151893	0.0
17	1.2173714029718471E-4	0.0	0.0	10.475480922572745	0.0
18	1.2173714029718471E-4	0.0	0.0	10.978194443429969	0.0
19	1.2173714029718471E-4	0.0	0.0	11.165060953786147	0.0
20	1.2173714029718471E-4	0.0	0.0	11.385405177724051	0.0
21	1.2173714029718471E-4	0.0	0.0	11.59388003048298	0.0
22	1.2173714029718471E-4	0.0	0.0	11.817206814358165	0.0
23	1.2173714029718471E-4	0.0	0.0	12.077480820313546	0.0
24	1.2173714029718471E-4	0.0	0.0	12.285833935932178	0.0
25	1.2173714029718471E-4	0.0	0.0	12.473613474840585	0.0
26	1.2173714029718471E-4	0.0	0.0	12.662427779441519	0.0
27	1.2173714029718471E-4	0.0	0.0	12.864572300904994	0.0
28	1.2173714029718471E-4	0.0	0.0	13.05332573693578	0.0
29	1.2173714029718471E-4	0.0	0.0	13.274643857996061	0.0
30	1.8260571044577707E-4	0.0	0.0	13.588360468541905	0.0
31	1.8260571044577707E-4	0.0	0.0	13.813574178091697	0.0
32	1.8260571044577707E-4	0.0	0.0	14.02667504218192	0.0
33	1.8260571044577707E-4	0.0	0.0	14.243671494761651	0.0
34	1.8260571044577707E-4	0.0	0.0	14.465841775804012	0.0
35	1.8260571044577707E-4	0.0	0.0	14.699759690885053	0.0
36	1.8260571044577707E-4	0.0	0.0	14.929355937485544	0.0
37	1.8260571044577707E-4	0.0	0.0	15.160352161199452	0.0
38	1.8260571044577707E-4	0.0	0.0	15.391165779202915	0.0
39	1.8260571044577707E-4	0.0	0.0	15.630379259886881	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCATCG	110	0.0	45.0	21
CCCGTAC	25	3.89154E-5	45.0	40
ACTCGTC	25	3.89154E-5	45.0	16
ACGTCGG	25	3.89154E-5	45.0	2
TATCGCG	40	6.8175723E-9	45.0	1
CAATACG	20	7.0341735E-4	45.0	1
ATCGATC	20	7.0341735E-4	45.0	37
CGTTTTT	27830	0.0	44.029823	1
AATACGG	115	0.0	43.04348	2
CGTTGAT	75	0.0	42.000004	25
CGTTCGG	370	0.0	40.743244	45
CGGGTAC	145	0.0	40.344826	6
TATACGG	95	0.0	40.26316	2
CGACGGT	185	0.0	40.135136	28
GTTTACG	45	1.9292202E-8	40.0	1
CGTTAGG	220	0.0	39.886364	2
CGTTCTG	435	0.0	39.827583	1
GTTTTTT	30045	0.0	39.59311	2
GTATACG	40	3.4594268E-7	39.375	1
GGTACGT	150	0.0	39.000004	9
>>END_MODULE