FastQCFastQC Report
Thu 26 May 2016
SRR1546579_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546579_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3135000
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT943893.0108133971291866No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTT63980.20408293460925042No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCT53590.17094098883572567No Hit
GAATCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTC52690.16807017543859648No Hit
CGTTCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTC45300.1444976076555024No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC38810.12379585326953747No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA34340.10953748006379586No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGACG351.2128294E-745.01
CGTTTTT462350.044.162971
CGACGGT2150.042.90697528
GACGGTC2300.040.10869229
GCGCGAC6250.039.69
TACGGGA9550.039.5811544
GTTTTTT507250.039.3267632
CCGCATC2250.039.00000420
TTCGCGG2150.038.7209322
CGGTCTA2450.038.5714331
ACGGGAT9000.038.5000045
GCGGCTA7000.038.2524
GCCGCAT2300.038.15217219
CGCATCG2250.038.00000421
TAGGGCG8750.037.85
CGGCTAC7100.037.7112725
GCCGACC1850.037.702714
TAGGGAC26900.037.555765
CGCGTCA601.5643309E-1037.49999639
GGCGCGA6500.037.3846178
Produced by FastQC (version 0.11.5)