##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546579_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3135000
Sequences flagged as poor quality	0
Sequence length	51
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.374610845295056	31.0	31.0	33.0	30.0	34.0
2	31.7286341307815	31.0	31.0	34.0	30.0	34.0
3	31.605501435406698	31.0	31.0	34.0	30.0	34.0
4	35.51339138755981	37.0	35.0	37.0	33.0	37.0
5	35.47966889952153	37.0	35.0	37.0	33.0	37.0
6	35.54105454545454	37.0	35.0	37.0	33.0	37.0
7	35.984276555023925	37.0	35.0	37.0	35.0	37.0
8	34.67091547049442	37.0	35.0	37.0	33.0	37.0
9	36.95651642743222	39.0	37.0	39.0	33.0	39.0
10	36.85864370015949	39.0	37.0	39.0	32.0	39.0
11	36.75562870813397	39.0	37.0	39.0	32.0	39.0
12	36.627052950558216	39.0	35.0	39.0	32.0	39.0
13	36.58781818181818	39.0	35.0	39.0	32.0	39.0
14	37.756364593301434	40.0	36.0	41.0	33.0	41.0
15	37.90881339712919	40.0	36.0	41.0	33.0	41.0
16	37.92725741626794	40.0	36.0	41.0	33.0	41.0
17	37.8142370015949	40.0	36.0	41.0	33.0	41.0
18	37.58391515151515	39.0	36.0	41.0	33.0	41.0
19	37.3645649122807	38.0	36.0	41.0	33.0	41.0
20	37.0284293460925	38.0	35.0	41.0	32.0	41.0
21	36.94998532695375	38.0	35.0	41.0	32.0	41.0
22	36.86713907496013	38.0	35.0	41.0	32.0	41.0
23	36.77208038277512	38.0	35.0	41.0	32.0	41.0
24	36.606994258373206	38.0	35.0	40.0	31.0	41.0
25	36.532601594896335	38.0	35.0	40.0	31.0	41.0
26	36.412531738437	38.0	35.0	40.0	31.0	41.0
27	36.26297543859649	38.0	35.0	40.0	31.0	41.0
28	36.23039170653907	38.0	35.0	40.0	31.0	41.0
29	36.14879872408294	38.0	35.0	40.0	30.0	41.0
30	36.02529696969697	38.0	35.0	40.0	30.0	41.0
31	35.78069601275917	38.0	35.0	40.0	30.0	41.0
32	35.362880701754385	38.0	35.0	40.0	26.0	41.0
33	34.89604784688995	38.0	34.0	40.0	23.0	41.0
34	34.44776363636364	38.0	34.0	40.0	21.0	41.0
35	34.11308612440192	38.0	34.0	40.0	18.0	41.0
36	33.918868899521534	38.0	33.0	40.0	16.0	41.0
37	33.8409288676236	38.0	33.0	40.0	15.0	41.0
38	33.73013684210526	38.0	33.0	40.0	15.0	41.0
39	33.63515023923445	38.0	33.0	40.0	15.0	41.0
40	33.533314832535886	37.0	33.0	40.0	15.0	41.0
41	33.43447336523126	37.0	33.0	40.0	15.0	41.0
42	33.35462711323764	37.0	33.0	40.0	13.0	41.0
43	33.26988133971292	37.0	33.0	40.0	12.0	41.0
44	33.15969314194577	37.0	33.0	40.0	12.0	41.0
45	33.06666315789474	37.0	33.0	40.0	10.0	41.0
46	32.991765869218504	37.0	33.0	40.0	10.0	41.0
47	32.92244976076555	36.0	32.0	40.0	10.0	41.0
48	32.84597320574163	36.0	32.0	40.0	10.0	41.0
49	32.78484848484848	36.0	32.0	40.0	10.0	41.0
50	32.691678468899525	36.0	32.0	40.0	10.0	41.0
51	32.38159489633174	35.0	31.0	40.0	10.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	5.0
10	2.0
11	5.0
12	3.0
13	19.0
14	30.0
15	105.0
16	314.0
17	850.0
18	2052.0
19	4179.0
20	7288.0
21	11494.0
22	17592.0
23	26788.0
24	41606.0
25	66601.0
26	92596.0
27	95458.0
28	84659.0
29	77282.0
30	79273.0
31	90084.0
32	107634.0
33	134090.0
34	197416.0
35	308330.0
36	256986.0
37	288180.0
38	404074.0
39	739408.0
40	597.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.18701754385965	23.019840510366826	25.284114832535888	23.50902711323764
2	40.53145135566188	23.078819776714514	25.012312599681017	11.377416267942584
3	24.864306220095695	23.241945773524723	39.78079744816586	12.112950558213715
4	21.142966507177032	25.372440191387557	39.83444976076555	13.650143540669857
5	20.13792663476874	29.086730462519938	37.97757575757576	12.797767145135566
6	17.645390749601276	37.171993620414675	35.60877192982456	9.57384370015949
7	76.07827751196172	3.2865390749601278	18.735279106858055	1.8999043062200955
8	73.55282296650718	6.122583732057416	17.417448165869217	2.9071451355661884
9	70.43508771929825	5.468006379585327	20.268197767145136	3.8287081339712916
10	33.86896331738437	28.791483253588517	25.837735247208933	11.501818181818182
11	25.192185007974484	23.76701754385965	36.13001594896332	14.910781499202553
12	22.945805422647528	21.5652312599681	38.67371610845295	16.81524720893142
13	19.493811802232855	26.785582137161086	39.441818181818185	14.27878787878788
14	16.041371610845296	31.01948963317384	37.70066985645933	15.238468899521532
15	14.647432216905901	25.357256778309413	45.48698564593302	14.508325358851677
16	16.51237639553429	23.884912280701755	43.497448165869216	16.105263157894736
17	16.65591706539075	24.390079744816585	37.781499202551835	21.17250398724083
18	17.875821371610844	25.362360446570975	39.5555980861244	17.20622009569378
19	20.175151515151516	27.41681020733652	35.297352472089315	17.110685805422648
20	21.219106858054225	26.08854864433812	34.64015948963318	18.05218500797448
21	19.335502392344498	27.71272727272727	36.496874003189795	16.454896331738436
22	18.489346092503986	24.902838915470493	35.79342902711324	20.81438596491228
23	16.476555023923446	28.924178628389154	35.47014354066986	19.12912280701754
24	16.929952153110047	27.22701754385965	39.35984051036683	16.483189792663477
25	17.19177033492823	27.607719298245613	37.28647527910686	17.9140350877193
26	16.14896331738437	29.508995215311007	36.12331738437002	18.21872408293461
27	17.399170653907493	28.481371610845297	36.21371610845295	17.90574162679426
28	16.10988835725678	27.5814673046252	39.06503987240829	17.243604465709726
29	16.40937799043062	25.940574162679425	38.00328548644338	19.64676236044657
30	17.70529505582137	27.509250398724085	36.673397129186604	18.112057416267945
31	19.878245614035087	27.053014354066985	34.10137161084529	18.967368421052633
32	19.577799043062203	28.237416267942585	33.60306220095694	18.581722488038277
33	18.581626794258373	28.423859649122807	33.12810207336523	19.866411483253586
34	16.27323763955343	29.516650717703346	33.4303668261563	20.779744816586923
35	16.908389154704945	28.124688995215312	33.48535885167464	21.481562998405103
36	19.465614035087718	29.827336523125997	30.875693779904307	19.83135566188198
37	17.244784688995214	30.681435406698565	32.674673046251996	19.39910685805423
38	17.307208931419456	32.32657097288676	30.404912280701758	19.961307814992026
39	17.838181818181816	30.928676236044655	30.455023923444973	20.77811802232855
40	19.0133971291866	28.866858054226473	31.23240829346092	20.887336523125995
41	17.296204146730464	28.679904306220095	31.17177033492823	22.85212121212121
42	18.703030303030303	30.083700159489634	29.831004784688997	21.38226475279107
43	18.921977671451355	29.443668261563	31.329473684210523	20.30488038277512
44	19.124082934609252	30.914928229665072	30.4888995215311	19.472089314194577
45	17.730558213716108	30.939043062200955	30.030940988835724	21.29945773524721
46	20.53818181818182	29.972727272727273	29.789665071770333	19.699425837320575
47	19.254960127591705	29.143381180223287	30.871929824561406	20.729728867623603
48	19.274704944178627	28.316937799043064	31.029696969696968	21.37866028708134
49	18.79671451355662	27.34031897926635	31.75323763955343	22.109728867623605
50	17.71425837320574	29.105741626794256	32.24427432216906	20.93572567783094
51	17.647783094098884	29.660350877192982	30.550749601275918	22.141116427432216
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4204.0
1	4772.0
2	5340.0
3	66129.0
4	126918.0
5	82544.5
6	38171.0
7	38932.5
8	39694.0
9	43434.5
10	47175.0
11	50043.0
12	52911.0
13	53605.0
14	54299.0
15	53067.0
16	51835.0
17	48905.0
18	45975.0
19	43409.0
20	40843.0
21	38775.5
22	36708.0
23	34816.0
24	32924.0
25	32526.5
26	35052.5
27	37976.0
28	41194.0
29	44412.0
30	48068.0
31	51724.0
32	58891.0
33	66058.0
34	74410.5
35	82763.0
36	90890.5
37	99018.0
38	110476.5
39	121935.0
40	146491.5
41	171048.0
42	195727.5
43	220407.0
44	256540.5
45	292674.0
46	289735.5
47	286797.0
48	268258.0
49	249719.0
50	225890.0
51	202061.0
52	181131.0
53	160201.0
54	143529.5
55	126858.0
56	112381.5
57	97905.0
58	85768.0
59	73631.0
60	61807.5
61	49984.0
62	41408.5
63	32833.0
64	28062.0
65	23291.0
66	18778.0
67	14265.0
68	11118.5
69	7972.0
70	6500.0
71	5028.0
72	4159.5
73	3291.0
74	2666.5
75	1495.0
76	948.0
77	677.0
78	406.0
79	350.0
80	294.0
81	239.0
82	184.0
83	135.5
84	87.0
85	59.5
86	32.0
87	21.0
88	10.0
89	8.0
90	6.0
91	6.5
92	7.0
93	5.0
94	3.0
95	3.0
96	3.0
97	2.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3135000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.390122210347148
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.72528274639228	23.620808414966486
2	7.539775542005225	4.582694003202505
3	3.307451904702272	3.015416027662426
4	1.9572627597572458	2.3792581786713614
5	1.301644405407754	1.977856627737815
6	0.9581251669121065	1.7470524549160908
7	0.724250421252877	1.5407041168939235
8	0.581364427220448	1.4134188793582307
9	0.4637119290403148	1.2683035974537893
>10	4.511437582249379	29.703283041656015
>50	0.7634759425820724	15.990188558522354
>100	0.16014609435526533	6.957609136192593
>500	0.003360775389420049	0.7036070026957824
>1k	0.002276654296058492	1.4290575829709233
>5k	3.2523632800835595E-4	0.5609483436925173
>10k+	1.0841210933611865E-4	3.1097940334073195
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	94389	3.0108133971291866	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTT	6398	0.20408293460925042	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCT	5359	0.17094098883572567	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTC	5269	0.16807017543859648	No Hit
CGTTCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTC	4530	0.1444976076555024	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3881	0.12379585326953747	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3434	0.10953748006379586	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.569377990430622E-5	0.0	0.0	0.0739712918660287	0.0
2	9.569377990430622E-5	0.0	0.0	0.2691228070175439	0.0
3	9.569377990430622E-5	0.0	0.0	0.48363636363636364	0.0
4	9.569377990430622E-5	0.0	0.0	0.8117703349282297	0.0
5	9.569377990430622E-5	0.0	0.0	1.5559170653907497	0.0
6	9.569377990430622E-5	0.0	0.0	2.656108452950558	0.0
7	9.569377990430622E-5	0.0	0.0	3.4279425837320576	0.0
8	9.569377990430622E-5	0.0	0.0	4.836108452950558	0.0
9	9.569377990430622E-5	0.0	0.0	5.629952153110048	0.0
10	9.569377990430622E-5	0.0	0.0	6.687049441786284	0.0
11	9.569377990430622E-5	0.0	0.0	7.290175438596491	0.0
12	9.569377990430622E-5	0.0	0.0	7.917671451355662	0.0
13	9.569377990430622E-5	0.0	0.0	8.243189792663477	0.0
14	9.569377990430622E-5	0.0	0.0	8.406634768740032	0.0
15	9.569377990430622E-5	0.0	0.0	8.556618819776714	0.0
16	9.569377990430622E-5	0.0	0.0	8.790877192982457	0.0
17	9.569377990430622E-5	0.0	0.0	9.060510366826156	0.0
18	9.569377990430622E-5	0.0	0.0	9.492886762360447	0.0
19	9.569377990430622E-5	0.0	0.0	9.689537480063796	0.0
20	9.569377990430622E-5	0.0	0.0	9.903062200956938	0.0
21	9.569377990430622E-5	0.0	0.0	10.122519936204148	0.0
22	9.569377990430622E-5	0.0	0.0	10.369473684210526	0.0
23	9.569377990430622E-5	0.0	0.0	10.644784688995216	0.0
24	9.569377990430622E-5	0.0	0.0	10.853014354066985	0.0
25	9.569377990430622E-5	2.2328548644338117E-4	0.0	11.03671451355662	0.0
26	9.569377990430622E-5	2.2328548644338117E-4	0.0	11.219170653907495	0.0
27	9.569377990430622E-5	2.2328548644338117E-4	0.0	11.42280701754386	0.0
28	9.569377990430622E-5	2.2328548644338117E-4	0.0	11.627304625199361	0.0
29	9.569377990430622E-5	2.2328548644338117E-4	0.0	11.848484848484848	0.0
30	9.569377990430622E-5	2.2328548644338117E-4	0.0	12.154609250398725	0.0
31	9.569377990430622E-5	2.2328548644338117E-4	0.0	12.389377990430622	0.0
32	9.569377990430622E-5	2.2328548644338117E-4	0.0	12.615406698564593	0.0
33	1.2759170653907497E-4	2.2328548644338117E-4	0.0	12.836937799043062	0.0
34	1.2759170653907497E-4	2.2328548644338117E-4	0.0	13.077384370015949	0.0
35	1.2759170653907497E-4	2.2328548644338117E-4	0.0	13.326315789473684	0.0
36	1.2759170653907497E-4	2.2328548644338117E-4	0.0	13.56842105263158	0.0
37	1.2759170653907497E-4	2.2328548644338117E-4	0.0	13.805199362041467	0.0
38	1.2759170653907497E-4	2.2328548644338117E-4	0.0	14.047113237639554	0.0
39	1.2759170653907497E-4	2.2328548644338117E-4	0.0	14.297735247208932	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCGACG	35	1.2128294E-7	45.0	1
CGTTTTT	46235	0.0	44.16297	1
CGACGGT	215	0.0	42.906975	28
GACGGTC	230	0.0	40.108692	29
GCGCGAC	625	0.0	39.6	9
TACGGGA	955	0.0	39.581154	4
GTTTTTT	50725	0.0	39.326763	2
CCGCATC	225	0.0	39.000004	20
TTCGCGG	215	0.0	38.720932	2
CGGTCTA	245	0.0	38.57143	31
ACGGGAT	900	0.0	38.500004	5
GCGGCTA	700	0.0	38.25	24
GCCGCAT	230	0.0	38.152172	19
CGCATCG	225	0.0	38.000004	21
TAGGGCG	875	0.0	37.8	5
CGGCTAC	710	0.0	37.71127	25
GCCGACC	185	0.0	37.7027	14
TAGGGAC	2690	0.0	37.55576	5
CGCGTCA	60	1.5643309E-10	37.499996	39
GGCGCGA	650	0.0	37.384617	8
>>END_MODULE