FastQCFastQC Report
Thu 26 May 2016
SRR1546578_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546578_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2914800
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT620492.128756689995883No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTT43390.14886098531631672No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCT32160.11033347056401811No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT447000.043.7164421
AGGGCGA32050.038.5413446
GTTTTTT497400.038.4680332
TAGGGCG13450.038.3085485
GACCGAT13250.037.188689
ATAGGGA31050.037.1739124
GGGCGAT62950.036.922167
GGCGATA11100.036.689198
CGTTCGG5650.036.6371745
CGTAAGG2950.036.610172
TAATGCG1300.036.3461571
GCGTTAG1800.036.2500041
TACGGGA8050.036.05594
TGGGCGA20100.036.0447776
CACGACC6250.036.027
GCGCAAC250.002107638636.09
TAGGGAC22600.035.9402665
CGGGTAC4150.035.783136
AAGGGAC22400.035.7589265
ATAGCGG4550.035.60442
Produced by FastQC (version 0.11.5)