##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546578_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2914800
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.422691093728556	31.0	31.0	33.0	30.0	34.0
2	31.77692397420063	31.0	31.0	34.0	30.0	34.0
3	31.661957938795116	31.0	31.0	34.0	30.0	34.0
4	35.569366680389734	37.0	35.0	37.0	33.0	37.0
5	35.54670646356525	37.0	35.0	37.0	33.0	37.0
6	35.599726910937285	37.0	35.0	37.0	33.0	37.0
7	36.012086935638806	37.0	35.0	37.0	35.0	37.0
8	34.70096473171401	37.0	35.0	37.0	33.0	37.0
9	36.973302113352545	39.0	37.0	39.0	33.0	39.0
10	36.88714079868259	39.0	37.0	39.0	32.0	39.0
11	36.87822835185948	39.0	37.0	39.0	32.0	39.0
12	36.80736036777824	39.0	35.0	39.0	32.0	39.0
13	36.82637024838754	39.0	37.0	39.0	33.0	39.0
14	38.00721250171539	40.0	37.0	41.0	33.0	41.0
15	38.094011252916154	40.0	37.0	41.0	33.0	41.0
16	38.07133731302319	40.0	37.0	41.0	33.0	41.0
17	37.94547447509263	40.0	37.0	41.0	33.0	41.0
18	37.70192088651022	39.0	36.0	41.0	33.0	41.0
19	37.46261904761905	39.0	36.0	41.0	33.0	41.0
20	37.141127693152185	39.0	35.0	41.0	32.0	41.0
21	37.070858377933305	39.0	35.0	41.0	32.0	41.0
22	36.9951118430081	38.0	35.0	41.0	32.0	41.0
23	36.90600555784273	38.0	35.0	41.0	32.0	41.0
24	36.745078221490324	38.0	35.0	41.0	32.0	41.0
25	36.64980135858378	38.0	35.0	40.0	31.0	41.0
26	36.56201008645533	38.0	35.0	40.0	31.0	41.0
27	36.44900644984219	38.0	35.0	40.0	31.0	41.0
28	36.39576368876081	38.0	35.0	40.0	31.0	41.0
29	36.28850693014958	38.0	35.0	40.0	31.0	41.0
30	36.14436942500343	38.0	35.0	40.0	30.0	41.0
31	35.87639117606697	38.0	35.0	40.0	30.0	41.0
32	35.42377864690545	38.0	35.0	40.0	25.0	41.0
33	34.86673836969946	38.0	34.0	40.0	22.0	41.0
34	34.30076677645121	38.0	33.0	40.0	18.0	41.0
35	33.92826163030053	38.0	33.0	40.0	15.0	41.0
36	33.72835666254974	38.0	33.0	40.0	15.0	41.0
37	33.650661451900646	38.0	33.0	40.0	14.0	41.0
38	33.5326320845341	38.0	33.0	40.0	12.0	41.0
39	33.41853437628654	38.0	33.0	40.0	12.0	41.0
40	33.31585975024015	38.0	33.0	40.0	10.0	41.0
41	33.18238198161109	38.0	33.0	40.0	10.0	41.0
42	33.11024152600522	37.0	32.0	40.0	10.0	41.0
43	33.01080691642651	37.0	32.0	40.0	10.0	41.0
44	32.88404796212433	37.0	32.0	40.0	10.0	41.0
45	32.78762659530671	37.0	32.0	40.0	10.0	41.0
46	32.7139724852477	37.0	31.0	40.0	10.0	41.0
47	32.66239570467957	37.0	31.0	40.0	10.0	41.0
48	32.578403320982574	36.0	31.0	40.0	10.0	41.0
49	32.50097159324825	36.0	31.0	40.0	10.0	41.0
50	32.402369630849456	36.0	31.0	40.0	10.0	41.0
51	32.04530259365994	35.0	31.0	40.0	10.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	1.0
11	5.0
12	7.0
13	15.0
14	32.0
15	119.0
16	283.0
17	783.0
18	1919.0
19	3792.0
20	6526.0
21	10275.0
22	15837.0
23	24053.0
24	38106.0
25	63443.0
26	92280.0
27	99511.0
28	88519.0
29	78288.0
30	76877.0
31	83638.0
32	97043.0
33	118486.0
34	171078.0
35	233532.0
36	222395.0
37	276764.0
38	403704.0
39	707021.0
40	465.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.352374090846713	20.85072732262934	24.09853163167284	24.698366954851107
2	42.119871003156305	21.607520241526004	24.28393028681213	11.988678468505558
3	26.0966447097571	22.039796898586523	39.61822423493893	12.245334156717442
4	22.339028406751748	24.52260875531769	39.48157678056813	13.656786057362428
5	20.149375600384246	28.228935089886097	38.37237546315356	13.249313846576094
6	17.68337450253877	36.30245642925758	36.23469877864691	9.779470289556745
7	76.24101139014684	3.1124605461781254	18.791272128447922	1.8552559352271167
8	73.71051187045423	5.580897488678469	18.060312885961302	2.648277754905997
9	70.3997186770962	5.057568272265678	20.964697406340058	3.5780156442980653
10	30.303211198023877	27.900713599560863	28.097433786194593	13.698641416220667
11	24.252881844380404	24.037463976945244	36.07163441745575	15.638019761218608
12	22.31727734321394	20.8364896390833	39.43618773157678	17.41004528612598
13	21.101550706738024	21.692671881432688	40.82420749279539	16.381569919033893
14	18.453684643886373	25.16923974200631	39.59825717030328	16.778818443804035
15	18.02041306436119	23.346335940716344	42.74722107863318	15.886029916289282
16	20.58292164127899	22.248147385755455	40.8320982571703	16.336832715795254
17	19.906854672704817	22.61788115822698	38.85851516399067	18.616749005077533
18	20.8888088376561	22.698675723891863	39.44527926444353	16.96723617400851
19	22.014546452586796	25.11599423631124	36.2107863318238	16.658672979278165
20	22.880986688623576	23.852579936873887	35.624365308082886	17.642068066419654
21	22.06923288047207	24.98960477562783	37.01684506655688	15.924317277343214
22	20.659290517359683	22.452998490462466	37.07702072183341	19.81069027034445
23	18.896699602031013	25.52428983120626	36.67243035542747	18.906580211335257
24	19.41155482365857	24.07108549471662	38.77278029367366	17.744579387951145
25	20.151262522299987	24.539316591189788	36.68766296143818	18.621757925072046
26	18.5414093591327	24.948572800878278	37.28863043776588	19.221387402223137
27	19.07451626183615	23.71233017702758	37.67088651022368	19.542267050912585
28	18.88496637848223	25.059695347879785	38.131432688349115	17.92390558528887
29	20.13160422670509	24.19449018800604	37.1547962124331	18.519109372855773
30	21.38997529847674	23.543364896390834	37.43526142445451	17.631398380677922
31	22.602339783175516	23.665911897900372	35.163887745299846	18.567860573624262
32	22.96445725264169	24.375120076849182	34.69277480444627	17.96764786606285
33	22.307499656923287	24.598634554686427	33.80564704267874	19.288218745711543
34	20.459860024701523	26.870660079593794	34.077501029230135	18.59197886647454
35	20.300603815013037	26.937388500068614	33.24303554274736	19.51897214217099
36	21.87398792369974	28.779676135583916	30.626801152737755	18.719534787978592
37	21.143989296006588	27.91285851516399	31.581858103471937	19.361294085357486
38	21.479964320021956	29.008096610402085	30.436496500617537	19.07544256895842
39	21.006792918896664	27.111602854398242	30.603163167284205	21.278441059420885
40	22.071600109784548	26.267085220255247	31.598943323727184	20.062371346233018
41	20.15829559489502	26.43797173047894	31.744167695896802	21.659564978729243
42	21.714079868258544	27.45244956772334	29.997426924660353	20.83604363935776
43	21.028989982160013	26.727425552353505	31.17740496775079	21.066179497735693
44	21.387539453821873	27.517256758611225	30.75785645670372	20.33734733086318
45	20.950974337861947	26.793330588719638	31.266913681899272	20.988781391519144
46	21.630609304240426	27.651777137367915	30.083436256346918	20.634177302044737
47	21.038184438040346	27.131741457389875	31.08460271716756	20.745471387402223
48	21.082372718539865	26.53911074516262	31.41141759297379	20.967098943323727
49	21.37048854123782	25.60412378207767	31.304926581583643	21.720461095100866
50	20.368430080966103	25.885343762865375	32.673974200631264	21.072251955537258
51	20.349595169479894	26.533175518045837	31.620145464525866	21.4970838479484
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2129.0
1	3330.5
2	4532.0
3	54721.5
4	104911.0
5	73369.5
6	41828.0
7	41550.5
8	41273.0
9	42534.0
10	43795.0
11	44294.0
12	44793.0
13	44300.0
14	43807.0
15	42303.0
16	40799.0
17	38531.0
18	36263.0
19	34551.0
20	32839.0
21	31060.5
22	29282.0
23	28701.0
24	28120.0
25	28961.0
26	31041.0
27	32280.0
28	35445.0
29	38610.0
30	43542.5
31	48475.0
32	53351.0
33	58227.0
34	66171.0
35	74115.0
36	81411.5
37	88708.0
38	95803.0
39	102898.0
40	114281.5
41	125665.0
42	137593.0
43	149521.0
44	163699.0
45	177877.0
46	198410.5
47	218944.0
48	224865.5
49	230787.0
50	221052.0
51	211317.0
52	193080.5
53	174844.0
54	159286.5
55	143729.0
56	132318.5
57	120908.0
58	112336.5
59	103765.0
60	94806.0
61	85847.0
62	75491.5
63	65136.0
64	57783.0
65	50430.0
66	41174.0
67	31918.0
68	26363.0
69	20808.0
70	17460.5
71	14113.0
72	11707.0
73	9301.0
74	7559.5
75	4639.5
76	3461.0
77	2581.0
78	1701.0
79	1219.0
80	737.0
81	568.0
82	399.0
83	271.0
84	143.0
85	111.5
86	80.0
87	58.0
88	36.0
89	24.0
90	12.0
91	11.0
92	10.0
93	6.5
94	3.0
95	2.5
96	2.0
97	1.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2914800.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.313173605029185
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.62940468599216	25.607717190304278
2	7.270847494862366	4.989717046938078
3	3.5723285619446083	3.6773379036062885
4	2.233993313402233	3.0662160158098075
5	1.6605910298051054	2.8490074146328386
6	1.2566747381623813	2.5872299073372234
7	1.0394286844923308	2.4966267830722715
8	0.8867066371141606	2.434057502082385
9	0.7963040129725324	2.4591346055557124
>10	6.498010811367648	41.463977367736796
>50	0.11776010483264997	2.6022293705237187
>100	0.03405967957895663	2.1087223885417514
>500	0.0023546222599518875	0.6078912617328955
>1k	0.0014332483321440044	0.8704735894675708
>5k	0.0	0.0
>10k+	1.0237488086742889E-4	2.1796616526583255
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	62049	2.128756689995883	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTT	4339	0.14886098531631672	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCT	3216	0.11033347056401811	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.430767119527926E-5	0.0	0.0	0.05338273637985454	0.0
2	3.430767119527926E-5	0.0	0.0	0.16224097708247565	0.0
3	3.430767119527926E-5	0.0	0.0	0.31751749691230957	0.0
4	3.430767119527926E-5	0.0	0.0	0.4779744750926307	0.0
5	3.430767119527926E-5	0.0	0.0	0.8410525593522712	0.0
6	3.430767119527926E-5	0.0	0.0	1.6439892960065872	0.0
7	3.430767119527926E-5	0.0	0.0	2.181796349663785	0.0
8	3.430767119527926E-5	0.0	0.0	3.29731027857829	0.0
9	3.430767119527926E-5	0.0	0.0	4.021030602442706	0.0
10	3.430767119527926E-5	0.0	0.0	4.93735419239742	0.0
11	3.430767119527926E-5	0.0	0.0	5.347811170577741	0.0
12	3.430767119527926E-5	0.0	0.0	5.762487992315082	0.0
13	3.430767119527926E-5	0.0	0.0	6.032969672018663	0.0
14	3.430767119527926E-5	0.0	0.0	6.176341429943736	0.0
15	3.430767119527926E-5	0.0	0.0	6.283449979415397	0.0
16	3.430767119527926E-5	0.0	0.0	6.434575271030602	0.0
17	3.430767119527926E-5	0.0	0.0	6.584122409770825	0.0
18	3.430767119527926E-5	0.0	0.0	6.854123782077672	0.0
19	3.430767119527926E-5	0.0	0.0	6.96257033072595	0.0
20	6.861534239055853E-5	0.0	0.0	7.101241937697269	0.0
21	6.861534239055853E-5	0.0	0.0	7.2283518594757785	0.0
22	6.861534239055853E-5	0.0	0.0	7.365925620968849	0.0
23	6.861534239055853E-5	0.0	0.0	7.52274598600247	0.0
24	6.861534239055853E-5	0.0	0.0	7.6570605187319885	0.0
25	6.861534239055853E-5	6.861534239055853E-5	0.0	7.776622752847537	0.0
26	6.861534239055853E-5	6.861534239055853E-5	0.0	7.897557293810896	0.0
27	1.029230135858378E-4	6.861534239055853E-5	0.0	8.042335666254974	0.0
28	1.029230135858378E-4	6.861534239055853E-5	0.0	8.180046658432826	0.0
29	1.029230135858378E-4	6.861534239055853E-5	0.0	8.326917798819816	0.0
30	1.029230135858378E-4	6.861534239055853E-5	0.0	8.53067105804858	0.0
31	1.029230135858378E-4	6.861534239055853E-5	0.0	8.687319884726225	0.0
32	1.029230135858378E-4	6.861534239055853E-5	0.0	8.848360093316865	0.0
33	1.029230135858378E-4	6.861534239055853E-5	0.0	9.020069987649238	0.0
34	1.029230135858378E-4	6.861534239055853E-5	0.0	9.18917249897077	0.0
35	1.029230135858378E-4	6.861534239055853E-5	0.0	9.368361465623714	0.0
36	1.029230135858378E-4	6.861534239055853E-5	0.0	9.544359818855495	0.0
37	1.3723068478111705E-4	6.861534239055853E-5	0.0	9.71679017428297	0.0
38	1.3723068478111705E-4	6.861534239055853E-5	0.0	9.927748044462742	0.0
39	1.3723068478111705E-4	6.861534239055853E-5	0.0	10.168965280636751	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	44700	0.0	43.716442	1
AGGGCGA	3205	0.0	38.541344	6
GTTTTTT	49740	0.0	38.468033	2
TAGGGCG	1345	0.0	38.308548	5
GACCGAT	1325	0.0	37.18868	9
ATAGGGA	3105	0.0	37.173912	4
GGGCGAT	6295	0.0	36.92216	7
GGCGATA	1110	0.0	36.68919	8
CGTTCGG	565	0.0	36.63717	45
CGTAAGG	295	0.0	36.61017	2
TAATGCG	130	0.0	36.346157	1
GCGTTAG	180	0.0	36.250004	1
TACGGGA	805	0.0	36.0559	4
TGGGCGA	2010	0.0	36.044777	6
CACGACC	625	0.0	36.0	27
GCGCAAC	25	0.0021076386	36.0	9
TAGGGAC	2260	0.0	35.940266	5
CGGGTAC	415	0.0	35.78313	6
AAGGGAC	2240	0.0	35.758926	5
ATAGCGG	455	0.0	35.6044	2
>>END_MODULE