FastQCFastQC Report
Thu 26 May 2016
SRR1546577_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546577_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1734970
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT528563.0465080087840137No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTT33740.1944702213871133No Hit
CGTTCTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTC26020.149973774762676No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCT25810.14876337919387655No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18710.10784048139160908No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG207.034281E-445.01
CGCGATA207.034281E-445.023
TTCGGCA2550.045.018
CGTTTTT295950.044.1332971
TGATTCG2750.042.54545615
CGTCGCC1100.040.90909227
CGTTCGG4000.040.545
GCGCGAC3400.040.3676459
TATAGCG900.040.01
ATTCGGC2950.039.6610217
CGTAAGG2500.039.62
GTCGTCG1200.039.37499625
AGGGCGA16250.039.323086
ACGCCGA1150.039.13043212
CCGCTCG1150.039.13043219
GTTTTTT325700.039.1211242
TCGCCGC1100.038.86363629
GCGCGTC356.2501294E-638.5714338
GTAAGCG659.094947E-1238.076921
TTCGAAT1300.038.0769216
Produced by FastQC (version 0.11.5)