FastQCFastQC Report
Thu 26 May 2016
SRR1546576_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546576_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3774680
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT482981.279525681647186No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC41210.1091748174679708No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT273450.042.992321
TAATACG800.042.18751
TCACGAC5300.038.63207625
AGGGCGA28400.038.345076
CGGTCTA5300.038.20754631
CGACGGT5350.037.85046828
CGAATAT3400.037.7205914
TAGGGAC34350.037.6637575
ACGGGAT18100.037.4171265
TAGGGAT56300.037.326825
CGAGACA18700.037.2994722
TATGGGA39700.037.292194
GTAGGGA48450.037.291024
GGGCGAT65650.037.2201087
CACGACC18950.037.1635927
TACGGGA14000.037.1254
CGTAAGG4200.036.9642872
CACAACG13300.036.87969612
AACACGT17700.036.86440741
GCGAGAC18800.036.86170221
Produced by FastQC (version 0.11.5)