##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546576_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3774680
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.409122892536587	31.0	31.0	33.0	30.0	34.0
2	31.770278540167645	31.0	31.0	34.0	30.0	34.0
3	31.806843758941156	31.0	31.0	34.0	30.0	34.0
4	35.6044284548624	37.0	35.0	37.0	33.0	37.0
5	35.54455450528257	37.0	35.0	37.0	33.0	37.0
6	35.59094333824324	37.0	35.0	37.0	33.0	37.0
7	36.0126241164814	37.0	35.0	37.0	35.0	37.0
8	34.70376137844797	37.0	35.0	37.0	33.0	37.0
9	36.98227319931756	39.0	37.0	39.0	33.0	39.0
10	36.858449457967296	39.0	37.0	39.0	32.0	39.0
11	36.79331678446915	39.0	37.0	39.0	32.0	39.0
12	36.61422186781396	39.0	35.0	39.0	32.0	39.0
13	36.54166631343584	39.0	35.0	39.0	32.0	39.0
14	37.64630061356194	40.0	36.0	41.0	32.0	41.0
15	37.79655546960272	40.0	36.0	41.0	33.0	41.0
16	37.83831371136096	40.0	36.0	41.0	33.0	41.0
17	37.72476050950014	40.0	36.0	41.0	33.0	41.0
18	37.62728337236534	39.0	36.0	41.0	33.0	41.0
19	37.52319905263492	39.0	36.0	41.0	33.0	41.0
20	37.3402155944345	39.0	35.0	41.0	32.0	41.0
21	37.23347700997171	39.0	35.0	41.0	32.0	41.0
22	37.15622410376509	39.0	35.0	41.0	32.0	41.0
23	37.093880275943924	39.0	35.0	41.0	32.0	41.0
24	36.96527122828955	39.0	35.0	41.0	32.0	41.0
25	36.85781443725031	38.0	35.0	41.0	31.0	41.0
26	36.77196504074517	38.0	35.0	41.0	31.0	41.0
27	36.66302123623724	38.0	35.0	41.0	31.0	41.0
28	36.64042329416004	38.0	35.0	41.0	31.0	41.0
29	36.599525787616436	38.0	35.0	41.0	31.0	41.0
30	36.52526147911876	38.0	35.0	41.0	31.0	41.0
31	36.401159303570104	38.0	35.0	41.0	30.0	41.0
32	36.213667383725245	38.0	35.0	41.0	30.0	41.0
33	35.99261500312609	38.0	35.0	41.0	29.0	41.0
34	35.775141204022596	38.0	35.0	41.0	27.0	41.0
35	35.60061780071423	38.0	35.0	41.0	27.0	41.0
36	35.46184418281815	38.0	35.0	41.0	26.0	41.0
37	35.4181035213581	38.0	35.0	41.0	25.0	41.0
38	35.319673720686254	38.0	35.0	41.0	25.0	41.0
39	35.224493996842114	38.0	35.0	40.0	25.0	41.0
40	35.117282524611355	38.0	34.0	40.0	24.0	41.0
41	35.03370802293175	38.0	34.0	40.0	24.0	41.0
42	34.966398741085335	38.0	34.0	40.0	24.0	41.0
43	34.873887322898895	38.0	34.0	40.0	23.0	41.0
44	34.75388642216029	38.0	34.0	40.0	23.0	41.0
45	34.65159669164009	38.0	34.0	40.0	23.0	41.0
46	34.58670854218106	37.0	34.0	40.0	23.0	41.0
47	34.527939322008756	37.0	34.0	40.0	23.0	41.0
48	34.45412723727574	37.0	34.0	40.0	23.0	41.0
49	34.39263619697564	37.0	34.0	40.0	23.0	41.0
50	34.29614430892155	37.0	34.0	40.0	23.0	41.0
51	33.961091801159306	36.0	33.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	3.0
10	5.0
11	7.0
12	5.0
13	19.0
14	53.0
15	103.0
16	314.0
17	773.0
18	1663.0
19	3330.0
20	5764.0
21	9236.0
22	14099.0
23	21107.0
24	31567.0
25	48083.0
26	66974.0
27	76737.0
28	77566.0
29	79452.0
30	88733.0
31	105405.0
32	128541.0
33	163970.0
34	251686.0
35	376412.0
36	292551.0
37	367825.0
38	549445.0
39	1012466.0
40	784.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.867819258851085	22.212240507804633	27.037682664490763	17.882257568853518
2	34.08641262305679	24.375152330793604	28.085586062924538	13.452848983225069
3	27.139731050049278	24.527615585956955	34.60182055167591	13.730832812317864
4	24.293291086926576	27.31908400182267	33.40288448292306	14.9847404283277
5	21.77326289910668	31.21938813356364	32.41445632477455	14.59289264255513
6	19.862478408765778	39.13174096877086	30.099531615925056	10.906249006538303
7	83.91911367321202	3.3678616465501707	10.776171754956712	1.9368529252810833
8	81.1575550775165	5.537025655154874	10.275493551771275	3.0299257155573454
9	77.91839308232751	5.229449913635063	13.310532283531321	3.541624720506109
10	35.28293789142391	32.852957071857745	19.143689001451776	12.720416035266565
11	27.4651361174987	24.33740078629182	31.963451206459887	16.234011889749596
12	26.497107039537127	21.992009918721585	32.90512043405004	18.605762607691247
13	22.029602509351786	27.426669280574778	33.78222789746415	16.761500312609282
14	18.08804984793413	30.59371920268738	33.59203959011095	17.72619135926754
15	16.782959085273454	24.932497589199613	41.05306939926033	17.231473926266595
16	19.992953045026333	23.067756736994923	38.79807029999894	18.141219917979804
17	18.76749817203048	23.175024108003857	33.36486801530196	24.692609704663706
18	21.08933737429398	23.3193807157163	35.60264711180815	19.988634798181568
19	23.346031981518962	26.126002734006594	30.638385240603178	19.88958004387127
20	24.72318183263217	25.239384530609215	30.07415198109509	19.963281655663526
21	22.122537539606007	27.135863172507342	32.08674642618712	18.654852861699535
22	21.517903504403023	23.01972617546388	31.785899731897803	23.676470588235293
23	18.769961957040067	27.909597634766392	31.04019413566183	22.280246272531713
24	19.293158625366917	25.435109731156018	35.9584123687306	19.31331927474647
25	19.718863585787403	26.248582661311687	33.389373403838206	20.6431803490627
26	18.710566193690592	28.496852713342584	30.994309451397207	21.798271641569617
27	19.280071423272965	26.813690167113503	32.33964203588119	21.566596373732345
28	17.940726101285406	27.012408998908516	35.09497493827291	19.951889961533162
29	18.5162980703	25.116433710937088	34.33446013966746	22.03280807909545
30	20.37152818252143	25.18679729142603	33.48516960378098	20.956504922271556
31	22.73435099134231	25.85869530662202	29.879910350016424	21.52704335201924
32	22.098985874299277	26.356830247862074	30.388827662212424	21.155356215626224
33	21.091430266936534	26.40115188572276	29.52287346212129	22.98454438521941
34	18.930955736645224	26.9135131984698	32.38274502739305	21.77278603749192
35	18.970747189165703	26.537216399800776	31.80084139582693	22.691195015206585
36	22.461506670764145	26.440466476628483	30.679660262591796	20.418366590015577
37	19.365032267635932	28.23513516377547	31.061493954454416	21.338338614134177
38	20.66522195258936	28.982774698782414	29.6378765882141	20.71412676041413
39	19.946485529899224	27.341920374707264	30.05102419277926	22.66056990261426
40	22.070877531340404	25.527885807538652	30.432301546091324	21.96893511502962
41	18.74768192270603	25.11227441796391	31.18200748142889	24.958036177901175
42	21.627184291118716	26.872715038095947	28.73070035075821	22.769400320027128
43	21.146428306505452	25.999475452223763	30.144912946263	22.70918329500779
44	20.601772865514427	27.928884037852214	29.985561689997564	21.483781406635792
45	19.81611686288639	28.07032649125224	29.023334428348896	23.090222217512476
46	21.896505134210052	27.639932391619954	29.05570803352867	21.407854440641323
47	20.740831010840655	27.13098858711202	30.209925079741858	21.918255322305466
48	21.00305191433446	26.48717242256297	30.334174022698612	22.175601640403954
49	21.098954083524962	25.052057392944565	30.79182871130798	23.057159812222494
50	20.039764960208547	26.44515561584028	31.898968919219644	21.616110504731527
51	19.77704070278805	27.01566755327604	29.75319762205008	23.45409412188583
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3773.0
1	4860.5
2	5948.0
3	41392.0
4	76836.0
5	51780.0
6	26724.0
7	26574.0
8	26424.0
9	27830.5
10	29237.0
11	29179.5
12	29122.0
13	29209.5
14	29297.0
15	27983.0
16	26669.0
17	25837.5
18	25006.0
19	24548.5
20	24091.0
21	24028.0
22	23965.0
23	26275.0
24	28585.0
25	31680.5
26	39452.0
27	44128.0
28	49072.0
29	54016.0
30	62756.0
31	71496.0
32	78609.0
33	85722.0
34	97082.5
35	108443.0
36	119692.5
37	130942.0
38	145397.0
39	159852.0
40	179426.5
41	199001.0
42	222478.0
43	245955.0
44	265263.5
45	284572.0
46	301812.5
47	319053.0
48	329591.5
49	340130.0
50	322926.5
51	305723.0
52	273184.5
53	240646.0
54	216638.5
55	192631.0
56	171903.0
57	151175.0
58	135107.5
59	119040.0
60	107628.5
61	96217.0
62	84172.5
63	72128.0
64	62788.5
65	53449.0
66	45502.0
67	37555.0
68	31476.5
69	25398.0
70	21313.5
71	17229.0
72	14566.0
73	11903.0
74	10063.5
75	6727.0
76	5230.0
77	3850.0
78	2470.0
79	1763.0
80	1056.0
81	781.5
82	507.0
83	362.5
84	218.0
85	138.0
86	58.0
87	38.5
88	19.0
89	19.5
90	20.0
91	13.5
92	7.0
93	7.0
94	7.0
95	6.5
96	6.0
97	3.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3774680.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.628765972959485
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.73557109336137	18.604243831123497
2	7.7144945927864335	3.6456797466522404
3	2.8839571859779127	2.0443304827052065
4	1.5608590765002897	1.4752469534158004
5	0.9475452236857274	1.11946621696328
6	0.6760465262530723	0.9584487093399633
7	0.5006412678096676	0.8280674747436259
8	0.41581202047927407	0.7860099936518563
9	0.3449086355673594	0.7334788888686515
>10	4.132120511938022	23.2036714621845
>50	1.5341839872123306	25.933459959346056
>100	0.5457343329138414	17.299946545468643
>500	0.005107485752074002	0.8208403783260334
>1k	0.0029019805409509925	1.2223875811426117
>5k	0.0	0.0
>10k+	1.160792216380397E-4	1.324721776068074
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	48298	1.279525681647186	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	4121	0.1091748174679708	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.94769357932328E-5	0.0	0.0	0.08660336770269268	0.0
2	7.94769357932328E-5	0.0	0.0	0.2844214608920491	0.0
3	7.94769357932328E-5	0.0	0.0	0.4703709983362828	0.0
4	7.94769357932328E-5	0.0	0.0	0.7433477804741064	0.0
5	7.94769357932328E-5	0.0	0.0	1.2400521368698803	0.0
6	7.94769357932328E-5	0.0	0.0	2.1353333262687166	0.0
7	7.94769357932328E-5	0.0	0.0	2.679644367204637	0.0
8	7.94769357932328E-5	0.0	0.0	3.947460446978287	0.0
9	7.94769357932328E-5	0.0	0.0	4.590322888297816	0.0
10	7.94769357932328E-5	0.0	0.0	5.4484088717454195	0.0
11	7.94769357932328E-5	0.0	0.0	6.032405395954094	0.0
12	7.94769357932328E-5	0.0	0.0	6.5733519132747675	0.0
13	7.94769357932328E-5	0.0	0.0	6.834089247300433	0.0
14	7.94769357932328E-5	0.0	0.0	6.954125912660146	0.0
15	7.94769357932328E-5	0.0	0.0	7.076679347653311	0.0
16	1.059692477243104E-4	0.0	0.0	7.298684921635742	0.0
17	1.059692477243104E-4	0.0	0.0	7.5589189017347165	0.0
18	1.059692477243104E-4	0.0	0.0	7.9513760106817	0.0
19	1.059692477243104E-4	0.0	0.0	8.130225608528406	0.0
20	1.059692477243104E-4	0.0	0.0	8.321526592982716	0.0
21	1.059692477243104E-4	0.0	0.0	8.543426197717423	0.0
22	1.059692477243104E-4	0.0	0.0	8.787181959795268	0.0
23	1.059692477243104E-4	0.0	0.0	9.056635264446257	0.0
24	1.32461559655388E-4	0.0	0.0	9.261367851049625	0.0
25	1.32461559655388E-4	0.0	0.0	9.440296925832124	0.0
26	1.32461559655388E-4	0.0	0.0	9.608841014337639	0.0
27	1.32461559655388E-4	0.0	0.0	9.79203535134104	0.0
28	1.32461559655388E-4	0.0	0.0	9.98919113673212	0.0
29	1.32461559655388E-4	0.0	0.0	10.198083016308667	0.0
30	1.32461559655388E-4	0.0	0.0	10.476066845401464	0.0
31	1.32461559655388E-4	0.0	0.0	10.68959487956595	0.0
32	1.32461559655388E-4	0.0	0.0	10.904023652336091	0.0
33	1.32461559655388E-4	0.0	0.0	11.12030688694141	0.0
34	1.32461559655388E-4	0.0	0.0	11.333887905729757	0.0
35	1.32461559655388E-4	0.0	0.0	11.56874225099876	0.0
36	1.32461559655388E-4	0.0	0.0	11.778746807676413	0.0
37	1.32461559655388E-4	0.0	0.0	12.005441520870644	0.0
38	1.32461559655388E-4	0.0	0.0	12.23939512753399	0.0
39	1.32461559655388E-4	0.0	0.0	12.512848771286572	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	27345	0.0	42.99232	1
TAATACG	80	0.0	42.1875	1
TCACGAC	530	0.0	38.632076	25
AGGGCGA	2840	0.0	38.34507	6
CGGTCTA	530	0.0	38.207546	31
CGACGGT	535	0.0	37.850468	28
CGAATAT	340	0.0	37.72059	14
TAGGGAC	3435	0.0	37.663757	5
ACGGGAT	1810	0.0	37.417126	5
TAGGGAT	5630	0.0	37.32682	5
CGAGACA	1870	0.0	37.29947	22
TATGGGA	3970	0.0	37.29219	4
GTAGGGA	4845	0.0	37.29102	4
GGGCGAT	6565	0.0	37.220108	7
CACGACC	1895	0.0	37.16359	27
TACGGGA	1400	0.0	37.125	4
CGTAAGG	420	0.0	36.964287	2
CACAACG	1330	0.0	36.879696	12
AACACGT	1770	0.0	36.864407	41
GCGAGAC	1880	0.0	36.861702	21
>>END_MODULE