FastQCFastQC Report
Thu 26 May 2016
SRR1546575_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546575_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2677009
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT205830.7688804931175054No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC33470.12502759609698735No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATACG253.8922008E-545.01
TATCACG302.1665892E-644.9999961
CGTTTTT135600.041.8473431
GGGCGAT54600.038.6538477
GACCGAT15800.038.1645559
AGGGCGA27400.037.4452556
TACGGGT2250.037.0000044
TAGGGAC17850.036.806725
CGTTCGG5600.036.56250445
CGACGGT2400.036.562528
CGGTCTA2400.036.562531
GGCGATA13550.036.3653148
TTAGGGA20500.036.2195134
CGTTAGG4000.036.02
TGGGCGA18100.035.4281736
CGTTCGA2100.035.35714314
GGACCGA16750.035.3283588
TAGCGGG16700.035.29943
TAGGGCG13950.035.1612935
GCGATAT3200.035.156259
Produced by FastQC (version 0.11.5)