##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546575_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2677009
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.444863651933932	31.0	31.0	33.0	30.0	34.0
2	31.809383158592293	31.0	31.0	34.0	30.0	34.0
3	31.886116931246775	31.0	31.0	34.0	30.0	34.0
4	35.663104606671105	37.0	35.0	37.0	33.0	37.0
5	35.60296958284414	37.0	35.0	37.0	33.0	37.0
6	35.649122584197514	37.0	35.0	37.0	33.0	37.0
7	35.99848637042311	37.0	35.0	37.0	35.0	37.0
8	34.6535738206334	37.0	35.0	37.0	33.0	37.0
9	36.94178129397398	39.0	37.0	39.0	33.0	39.0
10	36.883871888364965	39.0	37.0	39.0	32.0	39.0
11	36.836437606298674	39.0	37.0	39.0	32.0	39.0
12	36.56800780273806	39.0	35.0	39.0	32.0	39.0
13	36.48130955106987	39.0	35.0	39.0	32.0	39.0
14	37.590611387559775	40.0	36.0	41.0	32.0	41.0
15	37.739850332964885	40.0	36.0	41.0	33.0	41.0
16	37.78020245729469	40.0	36.0	41.0	33.0	41.0
17	37.679503132040274	40.0	36.0	41.0	33.0	41.0
18	37.584522876090446	39.0	36.0	41.0	33.0	41.0
19	37.49923515386015	39.0	36.0	41.0	32.0	41.0
20	37.3457810563954	39.0	35.0	41.0	32.0	41.0
21	37.241143380541494	39.0	35.0	41.0	32.0	41.0
22	37.15429944389429	39.0	35.0	41.0	32.0	41.0
23	37.07754101685874	39.0	35.0	41.0	32.0	41.0
24	36.93187695670803	39.0	35.0	41.0	31.0	41.0
25	36.830546703429086	38.0	35.0	41.0	31.0	41.0
26	36.713614709550846	38.0	35.0	41.0	31.0	41.0
27	36.592336447131856	38.0	35.0	41.0	31.0	41.0
28	36.60111938361059	38.0	35.0	41.0	31.0	41.0
29	36.54626712125361	38.0	35.0	41.0	31.0	41.0
30	36.48918102255166	38.0	35.0	41.0	31.0	41.0
31	36.36414744963502	38.0	35.0	41.0	30.0	41.0
32	36.24188637393449	38.0	35.0	41.0	30.0	41.0
33	36.062166021854985	38.0	35.0	41.0	30.0	41.0
34	35.860210780016054	38.0	35.0	41.0	29.0	41.0
35	35.72074692315192	38.0	35.0	41.0	28.0	41.0
36	35.59275108899522	38.0	35.0	41.0	27.0	41.0
37	35.54300639258217	38.0	35.0	41.0	27.0	41.0
38	35.43613301262715	38.0	35.0	41.0	27.0	41.0
39	35.353438856574634	38.0	35.0	40.0	26.0	41.0
40	35.22499774935385	38.0	34.0	40.0	26.0	41.0
41	35.14569655910757	38.0	34.0	40.0	25.0	41.0
42	35.06721979642205	38.0	34.0	40.0	25.0	41.0
43	34.96453280508209	38.0	34.0	40.0	25.0	41.0
44	34.84370653964929	37.0	34.0	40.0	24.0	41.0
45	34.718454812815345	37.0	34.0	40.0	24.0	41.0
46	34.644888007474016	37.0	34.0	40.0	24.0	41.0
47	34.56640302666147	37.0	34.0	40.0	23.0	41.0
48	34.480006977936945	37.0	34.0	40.0	23.0	41.0
49	34.40584697324514	36.0	34.0	40.0	24.0	41.0
50	34.294177569070555	36.0	34.0	40.0	24.0	41.0
51	33.93020083234685	36.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	4.0
11	7.0
12	7.0
13	13.0
14	36.0
15	88.0
16	222.0
17	533.0
18	1219.0
19	2350.0
20	4153.0
21	6879.0
22	10185.0
23	15157.0
24	21741.0
25	31706.0
26	42291.0
27	48132.0
28	51032.0
29	54813.0
30	62800.0
31	74521.0
32	91831.0
33	117696.0
34	189027.0
35	278965.0
36	211184.0
37	277716.0
38	402698.0
39	679478.0
40	522.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.24198088239524	22.950427137151948	26.010558799017858	15.797033181434953
2	34.986845393497	24.59020496382343	27.374058137271856	13.048891505407715
3	29.788992117695535	24.872721757752775	31.472998409792417	13.865287714759269
4	25.964611997942477	27.759749780445265	30.679015274136173	15.596622947476083
5	23.040340917792953	31.065976991485645	30.420816665166235	15.472865425555163
6	21.573069048329685	39.707972591799276	28.122542733326632	10.5964156265444
7	84.36452772478539	4.1472778014567755	9.30183648990347	2.1863579838543687
8	81.53614724492894	6.711632273182495	8.837736443919313	2.914484037969241
9	78.70436744889538	5.495312118861012	11.41736916088067	4.382951271362927
10	40.411108068743886	25.708990892447503	18.459146009595038	15.420755029213574
11	32.971499161937814	25.01851880214075	25.147169845151808	16.862812190769624
12	29.295194749065097	22.695142227762403	29.375172067034516	18.634490956137988
13	23.90085352720144	27.35015085866353	30.73370317395272	18.015292440182307
14	20.035868388937057	30.503446196856267	31.198064705796657	18.262620708410022
15	19.008639866358312	23.819419359441824	39.02004064984466	18.1519001243552
16	20.99649272751791	21.968995995157282	36.943506727097294	20.091004550227513
17	21.768884602181014	21.978633616846263	30.27995049699123	25.972531283981485
18	24.02121173294524	23.150687950619517	32.34057113741493	20.487529179020317
19	26.78634998985808	24.669323113967863	27.982498377853794	20.56182851832026
20	27.708386486560187	24.053972175663212	27.02019305874579	21.217448279030815
21	25.46326889450129	25.54130374608378	29.544017222205827	19.451410137209102
22	24.68217327621984	22.993086687418682	28.695047345750424	23.62969269061105
23	22.53836277726373	27.21649422919385	27.729043869482695	22.516099124059725
24	22.672654443821443	24.45423231673857	32.18890933874335	20.684203900696634
25	22.29125863977297	25.33939930721189	30.380996104234242	21.988345948780896
26	20.842664331722453	27.815035362227025	28.66404259380525	22.67825771224527
27	21.568661143836273	26.345410120025747	29.97942853385999	22.10650020227799
28	20.488575122459434	26.148660688103774	32.71961356872539	20.6431506207114
29	21.682556913331258	24.76898658166633	32.11774782976075	21.43070867524166
30	22.70638611973288	25.51803150456349	30.89608589287522	20.87949648282841
31	25.67100073253396	24.529166693126545	28.09011848671409	21.709714087625407
32	26.517990787479604	25.42505460385079	27.645966076318757	20.410988532350842
33	25.488483602408508	25.026662218916705	27.798300267201192	21.686553911473588
34	22.205752763625377	26.03842572064569	29.82705698785473	21.928764527874208
35	23.293160389076018	25.608356191555576	29.703187400565334	21.395296018803077
36	26.436145713368912	25.338614849632556	27.576261417126354	20.648978019872178
37	23.218898404898898	27.708909458279745	28.54443149051796	20.527760646303392
38	23.115574135163534	28.423886509160035	26.680971188367312	21.77956816730911
39	22.724279223566302	27.07779465814272	27.551607036061515	22.646319082229457
40	23.807204234277883	25.32173033411543	28.21006578610681	22.660999645499885
41	20.90396408827912	25.4533324318297	28.850482011827378	24.7922214680638
42	23.807540430383312	26.663488990884975	26.916980854378895	22.611989724352814
43	22.93993034763798	26.02135442951443	28.07319661607413	22.965518606773454
44	22.917143722714417	27.18029711517593	28.025867675454208	21.876691486655442
45	21.485060378952777	27.911112738134236	27.49501402498086	23.10881285793212
46	23.28456871082615	26.76337658932039	28.300091632116292	21.651963067737164
47	23.21691858338915	26.263714466406352	28.6673298446139	21.852037105590604
48	23.13342988387413	24.96891119902847	29.521604148510523	22.37605476858688
49	22.391893340664897	24.619715510855585	29.587199744192116	23.401191404287395
50	21.67164921746621	27.34690096297771	28.726575069415155	22.25487475014092
51	22.07004160240029	27.992472195648205	27.08541510319913	22.852071098752376
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2477.0
1	3509.5
2	4542.0
3	19976.5
4	35411.0
5	24867.5
6	14324.0
7	14517.5
8	14711.0
9	15555.0
10	16399.0
11	16259.5
12	16120.0
13	16006.5
14	15893.0
15	15621.0
16	15349.0
17	14555.5
18	13762.0
19	14145.5
20	14529.0
21	14125.5
22	13722.0
23	14468.5
24	15215.0
25	17185.0
26	21143.5
27	23132.0
28	24569.0
29	26006.0
30	32918.5
31	39831.0
32	44647.0
33	49463.0
34	55855.0
35	62247.0
36	68184.0
37	74121.0
38	83740.5
39	93360.0
40	112755.0
41	132150.0
42	149501.0
43	166852.0
44	174145.0
45	181438.0
46	194274.0
47	207110.0
48	210476.5
49	213843.0
50	208151.5
51	202460.0
52	190160.5
53	177861.0
54	163932.5
55	150004.0
56	140893.5
57	131783.0
58	125473.5
59	119164.0
60	111134.0
61	103104.0
62	96433.5
63	89763.0
64	81121.5
65	72480.0
66	61483.0
67	50486.0
68	42374.5
69	34263.0
70	29762.5
71	25262.0
72	21289.0
73	17316.0
74	13693.0
75	7899.0
76	5728.0
77	4682.5
78	3637.0
79	2469.5
80	1302.0
81	967.5
82	633.0
83	437.5
84	242.0
85	181.0
86	120.0
87	86.0
88	52.0
89	72.5
90	93.0
91	53.0
92	13.0
93	9.0
94	5.0
95	5.5
96	6.0
97	3.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2677009.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.01755112613924
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.88721756847585	18.38808114707402
2	6.468102047646101	2.9775973914156006
3	2.221791409256964	1.5342059206256735
4	1.2398438345778064	1.1415267540329272
5	0.841616793121577	0.9685978782146625
6	0.6004243575479998	0.8292179008344235
7	0.5023990949148246	0.8094797797049638
8	0.40774377005441675	0.7508210458873839
9	0.35161627042701143	0.7284010933202523
>10	5.186409414059119	30.317137174032716
>50	2.0529465886073366	32.42872399697694
>100	0.23366910677776964	7.085397904120749
>500	0.004034428886404035	0.6066641051823597
>1k	0.0020172144432020174	0.637734407909666
>5k	0.0	0.0
>10k+	1.6810120360016812E-4	0.7964135006676771
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	20583	0.7688804931175054	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3347	0.12502759609698735	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.471024565102321E-5	0.0	0.0	0.047739846971003835	0.0
2	7.471024565102321E-5	0.0	0.0	0.15341748944437616	0.0
3	7.471024565102321E-5	0.0	0.0	0.27306594785448984	0.0
4	7.471024565102321E-5	0.0	0.0	0.41568780680229317	0.0
5	7.471024565102321E-5	0.0	0.0	0.7249135135518783	0.0
6	7.471024565102321E-5	0.0	0.0	1.2394056202276496	0.0
7	7.471024565102321E-5	0.0	0.0	1.5136295768897303	0.0
8	7.471024565102321E-5	0.0	0.0	2.1561003343657044	0.0
9	7.471024565102321E-5	0.0	0.0	2.5396627355380574	0.0
10	7.471024565102321E-5	0.0	0.0	3.1455254726450304	0.0
11	7.471024565102321E-5	0.0	0.0	3.5261741742369934	0.0
12	7.471024565102321E-5	0.0	0.0	3.900846056176875	0.0
13	7.471024565102321E-5	0.0	0.0	4.081121878932794	0.0
14	7.471024565102321E-5	0.0	0.0	4.166702465326041	0.0
15	7.471024565102321E-5	0.0	0.0	4.255570302527933	0.0
16	7.471024565102321E-5	0.0	0.0	4.402226514740891	0.0
17	7.471024565102321E-5	0.0	0.0	4.551647006042939	0.0
18	7.471024565102321E-5	0.0	0.0	4.786461308124104	0.0
19	1.1206536847653483E-4	0.0	0.0	4.901066824952774	0.0
20	1.1206536847653483E-4	0.0	0.0	5.0240772444171835	0.0
21	1.1206536847653483E-4	0.0	0.0	5.169538092699725	0.0
22	1.1206536847653483E-4	0.0	0.0	5.319108004493074	0.0
23	1.1206536847653483E-4	0.0	0.0	5.487206057207876	0.0
24	1.1206536847653483E-4	0.0	0.0	5.626690085838336	0.0
25	1.1206536847653483E-4	0.0	0.0	5.740174948982241	0.0
26	1.1206536847653483E-4	0.0	0.0	5.8503725613175	0.0
27	1.1206536847653483E-4	0.0	0.0	5.9677050021124325	0.0
28	1.1206536847653483E-4	0.0	0.0	6.090453935717063	0.0
29	1.1206536847653483E-4	0.0	0.0	6.222877846133502	0.0
30	1.1206536847653483E-4	0.0	0.0	6.410549983208872	0.0
31	1.1206536847653483E-4	0.0	0.0	6.554666047069696	0.0
32	1.1206536847653483E-4	0.0	0.0	6.708419732619502	0.0
33	1.1206536847653483E-4	0.0	0.0	6.854590328235728	0.0
34	1.1206536847653483E-4	0.0	0.0	7.003824043923648	0.0
35	1.4942049130204643E-4	0.0	0.0	7.1535807313311235	0.0
36	1.4942049130204643E-4	0.0	0.0	7.295455487822417	0.0
37	1.4942049130204643E-4	0.0	0.0	7.435537198418086	0.0
38	1.4942049130204643E-4	0.0	0.0	7.58738577270379	0.0
39	1.4942049130204643E-4	0.0	0.0	7.7805864679573356	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATATACG	25	3.8922008E-5	45.0	1
TATCACG	30	2.1665892E-6	44.999996	1
CGTTTTT	13560	0.0	41.847343	1
GGGCGAT	5460	0.0	38.653847	7
GACCGAT	1580	0.0	38.164555	9
AGGGCGA	2740	0.0	37.445255	6
TACGGGT	225	0.0	37.000004	4
TAGGGAC	1785	0.0	36.80672	5
CGTTCGG	560	0.0	36.562504	45
CGACGGT	240	0.0	36.5625	28
CGGTCTA	240	0.0	36.5625	31
GGCGATA	1355	0.0	36.365314	8
TTAGGGA	2050	0.0	36.219513	4
CGTTAGG	400	0.0	36.0	2
TGGGCGA	1810	0.0	35.428173	6
CGTTCGA	210	0.0	35.357143	14
GGACCGA	1675	0.0	35.328358	8
TAGCGGG	1670	0.0	35.2994	3
TAGGGCG	1395	0.0	35.161293	5
GCGATAT	320	0.0	35.15625	9
>>END_MODULE