FastQCFastQC Report
Thu 26 May 2016
SRR1546574_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546574_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences716905
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44380.6190499438558805No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC12110.16892056827613144No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCG7320.10210557884238497No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGATT302.1643536E-645.00000442
TTTACGG850.045.02
CGACGGT551.8189894E-1245.028
GCGCGTC351.211065E-745.038
CGCATCG351.211065E-745.021
TTCGTAC253.8893326E-545.033
TAAGTCG253.8893326E-545.01
TCAGGCG207.031509E-445.01
TCTACGT207.031509E-445.029
GTTCGCG207.031509E-445.01
AATTCGT253.8893326E-545.031
TATAGCG207.031509E-445.01
CGGTCTA551.8189894E-1245.031
TAAATCG207.031509E-445.023
TACGTTG253.8893326E-545.01
TACGTCC207.031509E-445.031
TGATTCG1050.042.85714715
CGTTTTT28550.042.0840641
CGGGTTT700.041.7857136
ACGGTCT603.6379788E-1241.25000430