FastQCFastQC Report
Thu 26 May 2016
SRR1546573_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546573_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2101652
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT507972.4170033859078477No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTT66000.31403867053156276No Hit
CGTTCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTC45120.21468825476339565No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCT43880.20878813428674203No Hit
CGCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTG36180.17215028939139304No Hit
CTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTGCT24060.1144813698937788TruSeq Adapter, Index 22 (95% over 22bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22680.10791510678266429No Hit
CCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTGC22520.107153800914709TruSeq Adapter, Index 20 (95% over 21bp)
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC22370.1064400766635009No Hit
CGTCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCT22250.10586909726253443No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGA207.0346217E-445.0000042
CGAGTAG302.1663636E-644.9999961
CGTTTTT294100.044.2579081
CGACGGT1250.041.428
AATCGGT501.0822987E-940.526
CGTTTCT9550.040.287961
CGATTAC451.929584E-840.010
GACGGTC1300.039.8076929
AGTGCGG3300.039.5454522
GCTACGC403.4600453E-739.37500443
CGTTCGG4200.039.10714345
GTTTTTT325150.039.021222
ACGCCGA1100.038.86363612
CCGCTCG1050.038.5714319
CGACAGG1400.038.5714262
TCTAGCG356.2507625E-638.5714261
CGTTTTC5800.038.405171
TGGGCGA11250.038.26
AGGGCGA21200.037.9952856
TATACGG1900.037.8947372