##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546573_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2101652
Sequences flagged as poor quality	0
Sequence length	51
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.368121839391108	31.0	31.0	33.0	30.0	34.0
2	31.712949622487454	31.0	31.0	34.0	30.0	34.0
3	31.605234834311293	31.0	31.0	34.0	30.0	34.0
4	35.49959127391214	37.0	35.0	37.0	33.0	37.0
5	35.46460260785325	37.0	35.0	37.0	33.0	37.0
6	35.53017959205425	37.0	35.0	37.0	33.0	37.0
7	35.98638880271329	37.0	35.0	37.0	35.0	37.0
8	34.6849145339	37.0	35.0	37.0	33.0	37.0
9	36.96231631116855	39.0	37.0	39.0	33.0	39.0
10	36.86225455023001	39.0	37.0	39.0	32.0	39.0
11	36.760701105606444	39.0	37.0	39.0	32.0	39.0
12	36.60022353843548	39.0	35.0	39.0	32.0	39.0
13	36.55223271978424	39.0	35.0	39.0	32.0	39.0
14	37.71910240134903	40.0	36.0	41.0	33.0	41.0
15	37.86350166440496	40.0	36.0	41.0	33.0	41.0
16	37.87917790385849	40.0	36.0	41.0	33.0	41.0
17	37.77996119243338	40.0	36.0	41.0	33.0	41.0
18	37.54850517592827	39.0	36.0	41.0	32.0	41.0
19	37.339787462434316	38.0	36.0	41.0	32.0	41.0
20	37.03957648554566	38.0	35.0	41.0	32.0	41.0
21	36.95897703330523	38.0	35.0	41.0	32.0	41.0
22	36.87054517113204	38.0	35.0	41.0	32.0	41.0
23	36.79136365107068	38.0	35.0	41.0	32.0	41.0
24	36.64260162957521	38.0	35.0	40.0	31.0	41.0
25	36.55335421849098	38.0	35.0	40.0	31.0	41.0
26	36.44072995909884	38.0	35.0	40.0	31.0	41.0
27	36.30542544626798	38.0	35.0	40.0	31.0	41.0
28	36.25868887903421	38.0	35.0	40.0	31.0	41.0
29	36.17585356662283	38.0	35.0	40.0	31.0	41.0
30	36.03463085230095	38.0	35.0	40.0	30.0	41.0
31	35.766128264812636	38.0	35.0	40.0	30.0	41.0
32	35.35662183843947	38.0	34.0	40.0	27.0	41.0
33	34.84546537676076	38.0	34.0	40.0	23.0	41.0
34	34.37499072158474	38.0	34.0	40.0	21.0	41.0
35	34.02526345941193	38.0	33.0	40.0	18.0	41.0
36	33.83208161960211	38.0	33.0	40.0	16.0	41.0
37	33.75536768218525	38.0	33.0	40.0	15.0	41.0
38	33.66445158380169	37.0	33.0	40.0	15.0	41.0
39	33.588934324046036	37.0	33.0	40.0	15.0	41.0
40	33.48708254268547	37.0	33.0	40.0	15.0	41.0
41	33.406520679922274	37.0	33.0	40.0	15.0	41.0
42	33.31370892992751	37.0	33.0	40.0	14.0	41.0
43	33.211116778610354	37.0	33.0	40.0	12.0	41.0
44	33.08694065430433	37.0	33.0	40.0	12.0	41.0
45	32.97707612868353	37.0	32.0	40.0	10.0	41.0
46	32.90054157396182	36.0	32.0	40.0	10.0	41.0
47	32.83495697670214	36.0	32.0	40.0	10.0	41.0
48	32.74701187446828	36.0	32.0	40.0	10.0	41.0
49	32.69911859813138	36.0	32.0	40.0	10.0	41.0
50	32.58946152835959	36.0	32.0	40.0	10.0	41.0
51	32.250870743586475	35.0	31.0	40.0	10.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	4.0
12	2.0
13	6.0
14	20.0
15	67.0
16	215.0
17	548.0
18	1393.0
19	2712.0
20	4745.0
21	7493.0
22	11436.0
23	17238.0
24	27406.0
25	44524.0
26	61793.0
27	65226.0
28	58586.0
29	53325.0
30	54898.0
31	62102.0
32	73445.0
33	91225.0
34	134758.0
35	209640.0
36	165863.0
37	192489.0
38	270119.0
39	489974.0
40	397.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.489087632015192	22.716320304217824	24.198677992360295	23.595914071406686
2	39.01178691810062	23.32998041540655	25.742273221256422	11.915959445236414
3	25.377369802422095	22.731165768642953	39.93425172197871	11.957212706956243
4	21.69445750295482	25.764303509810375	39.70329055428777	12.837948432947034
5	19.870654133034392	29.406533526958796	38.09931425373944	12.623498086267373
6	17.185052520588563	37.6834985049856	35.53409413166405	9.597354842761789
7	76.95560444831018	3.2970729692641787	17.66714946147126	2.080173120954373
8	74.76861059775834	5.549206053142957	16.704716099525516	2.977467249573193
9	71.66162618739924	5.055404034540447	19.66277004946585	3.620199728594458
10	34.644984041125745	29.18803874285562	24.80734203379056	11.359635182228075
11	25.61941748681513	23.7072074729784	36.288881318124986	14.384493722081487
12	24.092333078930288	21.56484517893543	37.85074788785203	16.49207385428225
13	19.3893660796364	26.81557175022316	39.24260534094132	14.552456829199127
14	16.217432762417374	30.978534981053002	37.36703317199993	15.436999084529694
15	14.455152422951087	25.41824241120795	45.61892263800097	14.507682527840004
16	16.967223879119857	23.902577591342432	43.28918393720749	15.841014592330223
17	17.113156697683536	24.22432448378704	37.335772049797015	21.326746768732406
18	18.439874917445895	25.67228066302128	38.99375348535343	16.8940909341794
19	20.75947873387221	27.846808129985362	34.55648223397594	16.837230902166485
20	21.724814574439534	26.20633672939193	33.664136593498824	18.40471210266971
21	19.647543932106743	28.28256057615628	35.3192631320504	16.75063235968657
22	18.687346906148118	25.587204732277275	35.34771693886524	20.377731422709374
23	16.783463675242142	29.313273558134266	34.43990727294528	19.463355493678307
24	17.650257987526004	26.88442234965637	38.71088077379128	16.754438889026346
25	17.934320239506825	27.48114340528308	36.663919621326464	17.920616733883634
26	16.53275613660111	30.01277090593495	35.21244240245293	18.24203055501101
27	17.49652178381578	28.45580524273286	35.65266752059808	18.39500545285328
28	16.398671140607483	27.680843450771107	39.01197724456761	16.9085081640538
29	17.394554379126514	26.230222700999022	37.634061205185255	18.741161714689206
30	19.038594400975995	27.79627645299983	36.14309124441154	17.022037901612634
31	21.44384512754728	26.76951274521186	33.20868535799457	18.57795676924629
32	21.921992794239962	27.578923627698593	32.797151954747974	17.70193162331347
33	19.945071781627025	28.776790829309512	32.529172289227716	18.74896509983575
34	17.574460472047704	29.209260143924876	32.8922200250089	20.324059359018523
35	17.154980938804332	29.449404563648024	33.235283481756255	20.160331015791385
36	20.144866990348547	29.73084982670775	30.3679200933361	19.756363089607603
37	17.940315523216974	31.086687995919398	31.988835449446434	18.98416103141719
38	17.60724420598653	31.879968710328825	30.090709594166874	20.422077489517772
39	17.942837348904575	30.492631510830527	30.05469031028924	21.50984082997566
40	19.73737802452547	28.75894772302931	30.202764301606543	21.300909950838673
41	17.684659496434232	27.655339704194603	30.504669659867574	24.15533113950359
42	19.542103069394933	29.58453635521009	28.971732713122822	21.901627862272154
43	19.388081375984225	28.730446334597737	30.477215067004433	21.404257222413605
44	19.701311159031086	30.912729605091616	29.576114409045832	19.809844826831462
45	18.140967201039942	31.448831680982387	29.749834891789888	20.66036622618778
46	20.70414131359521	30.7017527164345	28.915729150211355	19.678376819758935
47	19.862232186870138	28.944230538642934	30.362400625793423	20.831136648693505
48	19.877981702013464	27.989552980226982	30.72373542337171	21.408729894387843
49	19.458121515836112	26.852780574519475	30.986243202966048	22.702854706678366
50	18.48455405557152	29.02711771501657	31.390163547533085	21.098164681878824
51	18.02691406569689	29.8109296876933	29.972041041999343	22.190115204610468
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2742.0
1	2943.0
2	3144.0
3	38079.0
4	73014.0
5	49193.0
6	25372.0
7	26006.5
8	26641.0
9	29062.5
10	31484.0
11	33651.5
12	35819.0
13	36145.0
14	36471.0
15	35278.0
16	34085.0
17	32161.5
18	30238.0
19	28499.0
20	26760.0
21	24735.5
22	22711.0
23	22311.5
24	21912.0
25	22005.5
26	23840.5
27	25582.0
28	27210.5
29	28839.0
30	32703.0
31	36567.0
32	41551.0
33	46535.0
34	50358.5
35	54182.0
36	59700.0
37	65218.0
38	74193.5
39	83169.0
40	96547.5
41	109926.0
42	123710.0
43	137494.0
44	149016.5
45	160539.0
46	177220.0
47	193901.0
48	191697.0
49	189493.0
50	174746.0
51	159999.0
52	137533.5
53	115068.0
54	98919.5
55	82771.0
56	72815.5
57	62860.0
58	56023.0
59	49186.0
60	43236.5
61	37287.0
62	32262.5
63	27238.0
64	23247.5
65	19257.0
66	14951.0
67	10645.0
68	7990.0
69	5335.0
70	4340.0
71	3345.0
72	2623.5
73	1902.0
74	1647.5
75	951.0
76	509.0
77	458.0
78	407.0
79	353.5
80	300.0
81	197.5
82	95.0
83	77.5
84	60.0
85	47.0
86	34.0
87	27.5
88	21.0
89	11.0
90	1.0
91	0.5
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2101652.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.86644721524543
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.10038912730596	26.111162586547902
2	7.714711045499928	5.225397088065884
3	3.3957963508173807	3.4501067360603965
4	2.072139325122017	2.807039883075163
5	1.4018884224836092	2.373849013085242
6	1.0303770269764918	2.093712551754053
7	0.7991076124156532	1.8944055042622872
8	0.6156424301604484	1.66796974915954
9	0.5232092608777161	1.5947314934438475
>10	4.793468765585912	33.95185587789305
>50	0.4877698294109164	10.802867189184237
>100	0.061163295726158834	3.2576307792281467
>500	0.002168753808838089	0.4518030469462674
>1k	0.0018795866343263444	1.5070057847475096
>5k	1.4458358725587264E-4	0.32317114011546166
>10k+	1.4458358725587264E-4	2.487291576431076
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	50797	2.4170033859078477	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTT	6600	0.31403867053156276	No Hit
CGTTCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTC	4512	0.21468825476339565	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCT	4388	0.20878813428674203	No Hit
CGCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTG	3618	0.17215028939139304	No Hit
CTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTGCT	2406	0.1144813698937788	TruSeq Adapter, Index 22 (95% over 22bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2268	0.10791510678266429	No Hit
CCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTGC	2252	0.107153800914709	TruSeq Adapter, Index 20 (95% over 21bp)
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2237	0.1064400766635009	No Hit
CGTCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCT	2225	0.10586909726253443	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.516323349441297E-5	0.0	0.0	0.12375978515948406	0.0
2	9.516323349441297E-5	0.0	0.0	0.3676155709889173	0.0
3	9.516323349441297E-5	0.0	0.0	0.7132960166573724	0.0
4	9.516323349441297E-5	0.0	0.0	1.0147255587509254	0.0
5	9.516323349441297E-5	0.0	0.0	1.696950779672372	0.0
6	9.516323349441297E-5	0.0	0.0	2.934643794500707	0.0
7	9.516323349441297E-5	0.0	0.0	3.73820213812753	0.0
8	9.516323349441297E-5	0.0	0.0	5.252487091107376	0.0
9	9.516323349441297E-5	0.0	0.0	6.2090679141932155	0.0
10	9.516323349441297E-5	0.0	0.0	7.387426652937784	0.0
11	9.516323349441297E-5	0.0	0.0	8.008937730889794	0.0
12	9.516323349441297E-5	0.0	0.0	8.626785024352271	0.0
13	9.516323349441297E-5	0.0	0.0	8.988643219714778	0.0
14	9.516323349441297E-5	0.0	0.0	9.168739639102954	0.0
15	9.516323349441297E-5	0.0	0.0	9.326092045685964	0.0
16	9.516323349441297E-5	0.0	0.0	9.566902608043577	0.0
17	9.516323349441297E-5	0.0	0.0	9.828411173686224	0.0
18	9.516323349441297E-5	0.0	0.0	10.30013532211803	0.0
19	9.516323349441297E-5	0.0	0.0	10.491365839825052	0.0
20	9.516323349441297E-5	0.0	0.0	10.710288858478949	0.0
21	9.516323349441297E-5	0.0	0.0	10.914699484024949	0.0
22	9.516323349441297E-5	0.0	0.0	11.145042090698174	0.0
23	9.516323349441297E-5	0.0	0.0	11.402934453468033	0.0
24	9.516323349441297E-5	0.0	0.0	11.599922346801469	0.0
25	9.516323349441297E-5	0.0	0.0	11.76564911793199	0.0
26	9.516323349441297E-5	0.0	0.0	11.93508725516879	0.0
27	9.516323349441297E-5	0.0	0.0	12.145826235742168	0.0
28	9.516323349441297E-5	0.0	0.0	12.32906304183566	0.0
29	9.516323349441297E-5	0.0	0.0	12.542514174563628	0.0
30	9.516323349441297E-5	0.0	0.0	12.846941358512256	0.0
31	9.516323349441297E-5	0.0	0.0	13.057490012618645	0.0
32	9.516323349441297E-5	0.0	0.0	13.258284435291856	0.0
33	9.516323349441297E-5	0.0	0.0	13.463361203472315	0.0
34	9.516323349441297E-5	0.0	0.0	13.673529204644726	0.0
35	9.516323349441297E-5	0.0	0.0	13.899256394493475	0.0
36	9.516323349441297E-5	0.0	0.0	14.117275362429174	0.0
37	9.516323349441297E-5	0.0	0.0	14.338672625153926	0.0
38	9.516323349441297E-5	0.0	0.0	14.565636937038102	0.0
39	9.516323349441297E-5	0.0	0.0	14.804686979576067	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGCGCGA	20	7.0346217E-4	45.000004	2
CGAGTAG	30	2.1663636E-6	44.999996	1
CGTTTTT	29410	0.0	44.257908	1
CGACGGT	125	0.0	41.4	28
AATCGGT	50	1.0822987E-9	40.5	26
CGTTTCT	955	0.0	40.28796	1
CGATTAC	45	1.929584E-8	40.0	10
GACGGTC	130	0.0	39.80769	29
AGTGCGG	330	0.0	39.545452	2
GCTACGC	40	3.4600453E-7	39.375004	43
CGTTCGG	420	0.0	39.107143	45
GTTTTTT	32515	0.0	39.02122	2
ACGCCGA	110	0.0	38.863636	12
CCGCTCG	105	0.0	38.57143	19
CGACAGG	140	0.0	38.571426	2
TCTAGCG	35	6.2507625E-6	38.571426	1
CGTTTTC	580	0.0	38.40517	1
TGGGCGA	1125	0.0	38.2	6
AGGGCGA	2120	0.0	37.995285	6
TATACGG	190	0.0	37.894737	2
>>END_MODULE