FastQCFastQC Report
Thu 26 May 2016
SRR1546572_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546572_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1288074
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74850.5811001541836882No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC15510.12041233655830333No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTCTA2050.042.8048831
CGTTTTT49400.041.037451
CGTTCGG2600.040.6730845
TGTTACG950.040.2631571
TATAGCG451.9286745E-840.01
CGACGGT2200.039.88636428
CCAACGG403.4586628E-739.3752
TAGTACG403.4586628E-739.3751
CGTAAGG1500.039.02
GATTACG356.2488743E-638.571431
AGGGTAC5600.038.1696436
GCGTTAG950.037.8947371
AGGGCGA13850.037.689536
TAGGGTC2400.037.5000045
GTTACGG1450.037.241382
GTAGGGT6850.037.116794
GTTGATC8200.037.04268316
ATTAGCG552.750312E-936.818181
ATAGGGT4100.036.768294
TAGGGTA7050.036.702135
Produced by FastQC (version 0.11.5)