##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546572_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1288074
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.458504713238526	31.0	31.0	33.0	30.0	34.0
2	31.8230963438436	31.0	31.0	34.0	30.0	34.0
3	31.924554800422957	33.0	31.0	34.0	30.0	34.0
4	35.672630609732046	37.0	35.0	37.0	33.0	37.0
5	35.61138490490453	37.0	35.0	37.0	33.0	37.0
6	35.662282601775985	37.0	35.0	37.0	33.0	37.0
7	36.00910972506238	37.0	35.0	37.0	35.0	37.0
8	34.66676293442768	37.0	35.0	37.0	33.0	37.0
9	36.958736066406125	39.0	37.0	39.0	33.0	39.0
10	36.907433113314916	39.0	37.0	39.0	32.0	39.0
11	36.84954358212339	39.0	37.0	39.0	33.0	39.0
12	36.4788156581066	39.0	35.0	39.0	32.0	39.0
13	36.356849839372586	39.0	35.0	39.0	32.0	39.0
14	37.430327760672135	40.0	35.0	41.0	32.0	41.0
15	37.599748927468454	40.0	35.0	41.0	33.0	41.0
16	37.66409926758866	40.0	35.0	41.0	33.0	41.0
17	37.56976617803014	39.0	35.0	41.0	33.0	41.0
18	37.505293950502846	39.0	36.0	41.0	33.0	41.0
19	37.443244720412025	39.0	35.0	41.0	32.0	41.0
20	37.30862202016344	39.0	35.0	41.0	32.0	41.0
21	37.19822774157385	39.0	35.0	41.0	32.0	41.0
22	37.11921908213348	39.0	35.0	41.0	32.0	41.0
23	37.05543547963859	39.0	35.0	41.0	32.0	41.0
24	36.91968939672721	39.0	35.0	41.0	32.0	41.0
25	36.806061608261636	38.0	35.0	41.0	31.0	41.0
26	36.700991557938444	38.0	35.0	41.0	31.0	41.0
27	36.590280527361	38.0	35.0	41.0	31.0	41.0
28	36.61151300313491	38.0	35.0	41.0	31.0	41.0
29	36.572751254974484	38.0	35.0	41.0	31.0	41.0
30	36.50727675583856	38.0	35.0	41.0	31.0	41.0
31	36.39414428053047	38.0	35.0	41.0	31.0	41.0
32	36.3036285182373	38.0	35.0	41.0	30.0	41.0
33	36.19596156742547	38.0	35.0	41.0	30.0	41.0
34	36.04169092769515	38.0	35.0	41.0	30.0	41.0
35	35.93093098688429	38.0	35.0	41.0	30.0	41.0
36	35.809722112238894	38.0	35.0	41.0	29.0	41.0
37	35.74036274313432	38.0	35.0	41.0	29.0	41.0
38	35.64585342146491	38.0	35.0	40.0	29.0	41.0
39	35.582163757672305	38.0	35.0	40.0	28.0	41.0
40	35.44702012462017	38.0	35.0	40.0	27.0	41.0
41	35.37954806944322	38.0	34.0	40.0	27.0	41.0
42	35.30579221380138	38.0	34.0	40.0	27.0	41.0
43	35.20854236635473	37.0	34.0	40.0	27.0	41.0
44	35.072648776390174	37.0	34.0	40.0	27.0	41.0
45	34.96344697587251	37.0	34.0	40.0	26.0	41.0
46	34.91042207202381	37.0	34.0	40.0	26.0	41.0
47	34.83900536770403	37.0	34.0	40.0	26.0	41.0
48	34.77004038587845	36.0	34.0	40.0	26.0	41.0
49	34.71183099728742	36.0	34.0	40.0	26.0	41.0
50	34.58244169201459	36.0	34.0	40.0	26.0	41.0
51	34.2170931173209	36.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	2.0
11	1.0
12	7.0
13	6.0
14	22.0
15	34.0
16	109.0
17	222.0
18	541.0
19	1038.0
20	1841.0
21	2874.0
22	4562.0
23	6393.0
24	9261.0
25	13333.0
26	17383.0
27	20071.0
28	22014.0
29	24683.0
30	29511.0
31	35776.0
32	45101.0
33	58810.0
34	97231.0
35	151886.0
36	102057.0
37	130642.0
38	187922.0
39	324469.0
40	270.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.99884323416202	23.40812717281771	27.14285048840362	14.450179104616659
2	32.773815790086594	25.156939741039725	28.415137639607664	13.654106829266022
3	30.14562827912061	25.260505219420626	30.580075368340637	14.013791133118128
4	25.534557797145197	27.91943630567809	30.407569751427328	16.13843614574939
5	23.29082024790501	31.75912253488542	29.275724841895727	15.67433237531384
6	20.771710320990874	41.12698494030623	27.33592945746906	10.765375281233842
7	85.25076975391165	4.405647501618696	8.032768303684415	2.3108144407852342
8	82.18782461256107	6.79735791577192	7.509040629653266	3.505776842013735
9	79.29451258235164	5.565130574796169	10.695115342752047	4.445241500100149
10	42.35991099890224	25.158259540989103	17.28557520763559	15.196254252473071
11	35.683198325562046	24.339362490043275	24.13820945069926	15.839229733695422
12	31.505332768148413	22.32363979088158	28.084799475806516	18.08622796516349
13	23.735825736720095	30.17544023091841	28.887858927359762	17.20087510500173
14	19.40571737338072	33.1064053773308	29.635098604583277	17.852778644705193
15	18.229309806734705	23.82634848618946	40.15398183644729	17.79035987062855
16	20.10459026422395	21.530983468341105	38.23840866285633	20.12601760457862
17	19.886202190246834	22.371307859641604	29.6517901921784	28.090699757933162
18	23.309297447196357	23.66820539813706	32.28292784420771	20.739569310458872
19	26.937660413920316	25.47361409360021	26.733634868804124	20.85509062367535
20	28.13347680335136	24.871241869644134	25.418648307473017	21.576633019531485
21	24.31350993809362	26.915456720654245	28.080063723046965	20.690969618205166
22	23.34011865777898	24.223841176826795	26.878502322071558	25.557537843322663
23	21.44434248342875	28.525690294191175	26.20773340662105	23.822233815759034
24	21.802085905002354	24.931176314404297	32.71900527454168	20.547732506051673
25	21.366629556997502	25.66490745096943	30.395225740136052	22.57323725189702
26	19.68497151561168	28.681504323509365	28.152575084971826	23.48094907590713
27	21.22750711527443	27.510453591952018	28.329040101733288	22.932999191040267
28	19.98650698639985	27.106361901567766	32.70735998087066	20.19977113116172
29	20.801056461041835	24.75028608604785	31.801356133265635	22.64730131964468
30	22.54482273533974	26.045320377555946	30.746758338418445	20.66309854868587
31	26.363081624192397	25.539992267525	26.37767705892674	21.71924904935586
32	26.59909290925832	26.191740536646186	26.9986041174653	20.210562436630195
33	25.801700833958296	25.845021326414475	26.52619337087776	21.827084468749465
34	20.895771516232763	27.20635615655622	29.81552302119288	22.08234930601813
35	21.32920934666797	26.418047410319595	29.876621995320146	22.37612124769229
36	25.903325430060697	25.993071826618657	27.33142661058293	20.772176132737716
37	21.749604448191644	29.172004092932546	27.781866569777826	21.296524889097988
38	21.848201267939572	29.71886708372345	25.749219377147586	22.68371227118939
39	21.531915091834787	27.832640050183453	26.369835894521586	24.265608963460174
40	22.88113881655868	24.995846511924004	27.681561773624807	24.441452897892514
41	20.300075927314733	24.219648871105232	27.814162850892103	27.66611235068793
42	23.47435007616022	26.030569672239327	25.920094652946958	24.57498559865349
43	23.080583879497606	25.54635836139849	27.327467210734785	24.045590548369116
44	22.275040098627873	27.43180904202709	27.78636941666395	22.50678144268109
45	20.515048048481688	29.126742718197868	26.836423994273623	23.521785239046825
46	22.750400986278738	27.604625200104966	27.833882214841694	21.811091598774603
47	22.998135200306816	26.529221147232224	27.987677726590242	22.484965925870718
48	23.339497575449858	24.87341565779606	28.56947659839419	23.217610168359894
49	22.473786443946544	24.2116524361178	29.292726970655416	24.021834149280245
50	21.228050562312415	27.48902625159735	28.48042891945649	22.80249426663375
51	21.10880275512121	28.145510273478074	26.840771570577466	23.904915400823246
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1471.0
1	1937.5
2	2404.0
3	7710.5
4	13017.0
5	9121.0
6	5225.0
7	5372.5
8	5520.0
9	5886.5
10	6253.0
11	6237.0
12	6221.0
13	6301.5
14	6382.0
15	6205.5
16	6029.0
17	6094.5
18	6160.0
19	5639.0
20	5118.0
21	5786.0
22	6454.0
23	6774.5
24	7095.0
25	8168.0
26	9948.5
27	10656.0
28	11966.5
29	13277.0
30	15622.5
31	17968.0
32	20154.0
33	22340.0
34	25829.0
35	29318.0
36	32235.0
37	35152.0
38	40190.5
39	45229.0
40	56263.5
41	67298.0
42	79883.0
43	92468.0
44	100455.0
45	108442.0
46	110978.0
47	113514.0
48	110085.5
49	106657.0
50	98862.0
51	91067.0
52	85548.0
53	80029.0
54	73806.0
55	67583.0
56	63353.5
57	59124.0
58	55955.5
59	52787.0
60	49367.0
61	45947.0
62	42432.5
63	38918.0
64	35163.5
65	31409.0
66	27043.5
67	22678.0
68	19699.5
69	16721.0
70	14667.0
71	12613.0
72	10348.5
73	8084.0
74	6734.0
75	4510.5
76	3637.0
77	2592.5
78	1548.0
79	1204.5
80	861.0
81	645.0
82	429.0
83	326.5
84	224.0
85	144.5
86	65.0
87	46.5
88	28.0
89	21.5
90	15.0
91	14.0
92	13.0
93	7.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1288074.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.85356859141707
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.46562777841103	20.286164898377503
2	6.292184107418035	3.253508268219131
3	2.4077524250226316	1.867469774144202
4	1.3833117302187705	1.4305417880209124
5	1.001139630551992	1.2941516054030933
6	0.7488075495232478	1.1615608406022133
7	0.6264181576653373	1.1336601364276935
8	0.5375949633793076	1.111899860810182
9	0.42666365375296406	0.9927700233990191
>10	6.894415451372457	44.9833005443316
>50	1.1487657196896557	18.67222297140603
>100	0.06483703269986561	2.7318367101700476
>500	0.0012409001473522325	0.18976323819667595
>1k	9.306751105141744E-4	0.290820583981888
>5k	3.102250368380581E-4	0.6003287565097716
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7485	0.5811001541836882	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	1551	0.12041233655830333	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.08159469098825067	0.0
2	0.0	0.0	0.0	0.24012595549634572	0.0
3	0.0	0.0	0.0	0.3825867147384389	0.0
4	0.0	0.0	0.0	0.6079619649181647	0.0
5	0.0	0.0	0.0	1.0183421138847613	0.0
6	0.0	0.0	0.0	1.7739664025514061	0.0
7	0.0	0.0	0.0	2.1894704807332497	0.0
8	0.0	0.0	0.0	3.325740601859831	0.0
9	0.0	0.0	0.0	3.967008106677101	0.0
10	0.0	0.0	0.0	4.863307542889617	0.0
11	0.0	0.0	0.0	5.415682639351465	0.0
12	0.0	0.0	0.0	5.926522855053359	0.0
13	0.0	0.0	0.0	6.162767045992699	0.0
14	0.0	0.0	0.0	6.2651679950064985	0.0
15	0.0	0.0	0.0	6.363066097134171	0.0
16	0.0	0.0	0.0	6.550632960528665	0.0
17	0.0	0.0	0.0	6.755745399720824	0.0
18	0.0	0.0	0.0	7.082124163673826	0.0
19	0.0	0.0	0.0	7.2256718169918805	0.0
20	0.0	0.0	0.0	7.381951658056913	0.0
21	0.0	0.0	0.0	7.560901004134855	0.0
22	0.0	0.0	0.0	7.74862313811163	0.0
23	0.0	0.0	0.0	7.96934027082295	0.0
24	0.0	0.0	0.0	8.135867970318476	0.0
25	0.0	0.0	0.0	8.270642835737698	0.0
26	0.0	0.0	0.0	8.408756018675946	0.0
27	0.0	0.0	0.0	8.554399824854784	0.0
28	0.0	0.0	0.0	8.695074972400654	0.0
29	0.0	0.0	0.0	8.848637578275783	0.0
30	0.0	0.0	0.0	9.059339758430028	0.0
31	0.0	0.0	0.0	9.220200081672326	0.0
32	0.0	0.0	0.0	9.38501980476277	0.0
33	0.0	0.0	0.0	9.553566021827939	0.0
34	0.0	0.0	0.0	9.71737648613356	0.0
35	0.0	0.0	0.0	9.903623549578674	0.0
36	0.0	0.0	0.0	10.062465355251328	0.0
37	0.0	0.0	0.0	10.238386924974808	0.0
38	0.0	0.0	0.0	10.419742965078093	0.0
39	0.0	0.0	0.0	10.654434450194632	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGTCTA	205	0.0	42.80488	31
CGTTTTT	4940	0.0	41.03745	1
CGTTCGG	260	0.0	40.67308	45
TGTTACG	95	0.0	40.263157	1
TATAGCG	45	1.9286745E-8	40.0	1
CGACGGT	220	0.0	39.886364	28
CCAACGG	40	3.4586628E-7	39.375	2
TAGTACG	40	3.4586628E-7	39.375	1
CGTAAGG	150	0.0	39.0	2
GATTACG	35	6.2488743E-6	38.57143	1
AGGGTAC	560	0.0	38.169643	6
GCGTTAG	95	0.0	37.894737	1
AGGGCGA	1385	0.0	37.68953	6
TAGGGTC	240	0.0	37.500004	5
GTTACGG	145	0.0	37.24138	2
GTAGGGT	685	0.0	37.11679	4
GTTGATC	820	0.0	37.042683	16
ATTAGCG	55	2.750312E-9	36.81818	1
ATAGGGT	410	0.0	36.76829	4
TAGGGTA	705	0.0	36.70213	5
>>END_MODULE