##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546571_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1202898
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.522743408003006	31.0	31.0	33.0	30.0	34.0
2	31.88116781306478	31.0	31.0	34.0	30.0	34.0
3	31.96547587576004	33.0	31.0	34.0	30.0	34.0
4	35.6980932714162	37.0	35.0	37.0	33.0	37.0
5	35.6305705055624	37.0	35.0	37.0	33.0	37.0
6	35.6731692961498	37.0	35.0	37.0	33.0	37.0
7	36.03051879710499	37.0	35.0	37.0	35.0	37.0
8	34.69609642712849	37.0	35.0	37.0	33.0	37.0
9	37.02439525213276	39.0	37.0	39.0	34.0	39.0
10	37.035853414005174	39.0	37.0	39.0	32.0	39.0
11	36.87101649516418	39.0	35.0	39.0	33.0	39.0
12	35.92599123117671	37.0	35.0	39.0	32.0	39.0
13	35.432527945012794	37.0	35.0	39.0	31.0	39.0
14	36.39870545964828	38.0	35.0	41.0	31.0	41.0
15	36.806055043736045	38.0	35.0	41.0	32.0	41.0
16	37.00104830168476	38.0	35.0	41.0	33.0	41.0
17	36.95552823265148	38.0	35.0	41.0	32.0	41.0
18	36.92803296705124	38.0	35.0	41.0	32.0	41.0
19	36.81983925486617	37.0	35.0	40.0	32.0	41.0
20	36.6396161603062	37.0	35.0	40.0	32.0	41.0
21	36.48309998021445	37.0	35.0	40.0	32.0	41.0
22	36.3745255208671	37.0	35.0	40.0	32.0	41.0
23	36.35410566814476	37.0	35.0	40.0	32.0	41.0
24	36.20237792398026	36.0	35.0	40.0	31.0	41.0
25	36.09333459694837	36.0	35.0	40.0	31.0	41.0
26	35.98486987259103	36.0	35.0	40.0	31.0	41.0
27	35.8586472003445	35.0	35.0	40.0	31.0	41.0
28	35.916174937525874	36.0	35.0	40.0	31.0	41.0
29	35.92929242545918	36.0	35.0	40.0	31.0	41.0
30	35.89198917946493	36.0	35.0	40.0	31.0	41.0
31	35.720425173206706	36.0	35.0	40.0	31.0	41.0
32	35.520750720343706	36.0	34.0	40.0	30.0	41.0
33	35.35282210129205	36.0	34.0	40.0	30.0	41.0
34	35.23375049256047	36.0	34.0	40.0	29.0	41.0
35	35.10879725463007	36.0	34.0	40.0	29.0	41.0
36	34.90659640302004	35.0	34.0	40.0	27.0	41.0
37	34.818927290593216	35.0	34.0	40.0	27.0	41.0
38	34.717138942786505	35.0	34.0	40.0	27.0	41.0
39	34.710536554221555	35.0	34.0	40.0	27.0	41.0
40	34.5143794403183	35.0	34.0	40.0	25.0	41.0
41	34.495840877613894	35.0	34.0	40.0	25.0	41.0
42	34.424715146255124	35.0	34.0	40.0	25.0	41.0
43	34.31243629966963	35.0	34.0	40.0	25.0	41.0
44	34.18739992917105	35.0	34.0	40.0	24.0	41.0
45	34.06440861984973	35.0	34.0	40.0	24.0	41.0
46	34.01837063491668	35.0	34.0	40.0	24.0	41.0
47	33.96290541675188	35.0	33.0	40.0	23.0	41.0
48	33.94903059112244	35.0	34.0	39.0	23.0	41.0
49	33.956260630577155	35.0	34.0	39.0	24.0	41.0
50	33.805372525351274	35.0	34.0	39.0	24.0	41.0
51	33.40958585017184	35.0	33.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	4.0
13	3.0
14	14.0
15	36.0
16	90.0
17	234.0
18	563.0
19	1053.0
20	1836.0
21	3022.0
22	4705.0
23	7096.0
24	10466.0
25	14806.0
26	19382.0
27	22111.0
28	23241.0
29	25906.0
30	29961.0
31	36686.0
32	46565.0
33	62054.0
34	117772.0
35	230900.0
36	80461.0
37	94571.0
38	135515.0
39	233599.0
40	246.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.84808853285981	23.523440890250047	29.15317840747927	14.475292169410872
2	30.626370648217886	26.89903882124669	29.441897816772496	13.032692713762927
3	27.745328365331055	25.638915352756424	31.96979295002569	14.645963331886827
4	24.464501562060956	28.66386011116487	31.58089879607415	15.290739530700026
5	22.600835648575355	32.44772208449927	30.35311389660636	14.598328370319013
6	20.22889721323005	40.93322958388824	27.66751628151348	11.17035692136823
7	84.37207477275712	5.559823027388855	7.879637342484566	2.188464857369453
8	82.3990063995451	7.0016742899231685	7.196537029739845	3.4027822807918877
9	80.39509584353786	5.8409773729775925	10.322737256192962	3.441189527291591
10	55.8343267675231	17.93236001722507	13.836584648074899	12.396728567176934
11	50.402527895133254	18.544631381879427	19.065540054102676	11.987300668884645
12	43.69913325984414	20.328822560183823	22.537821161894026	13.43422301807801
13	20.36889245804715	43.947699638705856	23.35842274241041	12.32498516083658
14	14.111504051050048	45.857753525236554	27.661530736604433	12.369211687108965
15	11.471795613593173	22.14285833046526	53.289056927520036	13.096289128421528
16	13.08573129226252	16.35350628232818	51.33776928717148	19.222993138237822
17	13.822867774325005	17.58312009829595	29.3985857487501	39.195426378628945
18	20.31809845888845	21.96320885062574	35.855076656541115	21.86361603394469
19	28.15267794941882	24.5795570364237	25.772592522391758	21.495172491765718
20	30.675169465740236	22.483203064598996	23.91100492311069	22.93062254655008
21	20.86585895063422	29.6799063594752	27.779662115989883	21.674572573900697
22	22.177192081124087	24.61397391965071	24.7871390591721	28.421694940053104
23	18.11974082590544	31.618391584323856	23.193238329434415	27.068629260336287
24	19.132628036624887	24.059230292177723	39.23815651867407	17.569985152523323
25	15.784879515968935	25.04867411867008	37.24904355980308	21.917402805557913
26	15.294813026540904	34.64142429366414	27.546724659946232	22.51703801984873
27	16.945908963187236	33.982848088532855	28.384700947212483	20.686542001067423
28	13.987802789596456	28.361922623530837	40.495952275255256	17.15432231161744
29	14.353752354729993	24.072614635654894	38.74468159395061	22.828951415664502
30	17.648545429454536	29.87984018595093	32.52644862656684	19.9451657580277
31	28.89671443464034	26.658286903794004	23.298401028183605	21.14659763338205
32	29.53841472843084	26.961970175359838	25.552124951575276	17.947490144634042
33	26.29807348586497	28.483711835916264	23.86470008263377	21.353514595584997
34	18.425502411675804	28.368656361553512	27.963883887079373	25.24195733969131
35	18.876080931217775	26.452700062681956	30.55462724187753	24.116591764222736
36	29.730866623770265	24.02007485256439	26.509396474181518	19.73966204948383
37	20.66110343520398	30.672509223558443	28.545063671233972	20.12132367000361
38	20.09746462293561	32.59794263520266	23.423848073568998	23.88074466829274
39	19.74340301505198	31.757222973186423	26.496344660977073	22.003029350784523
40	23.55212162627255	25.777497343914447	25.625531009279257	25.044850020533744
41	16.685537759643793	23.51670715222737	27.991068236874618	31.806686851254224
42	22.83385623718719	25.133053675373972	24.412709972084084	27.620380115354752
43	22.194816185578496	26.332157838819253	25.86719738498193	25.605828590620316
44	20.09887787659469	30.463597079719147	27.43241737869711	22.00510766498905
45	17.179760877480884	36.238899723833605	22.721461005006244	23.859878393679264
46	23.52793004893183	30.927559942738288	26.10587098823009	19.438639020099792
47	22.59485010366631	26.153505949797907	27.03479430508655	24.216849641449233
48	23.490187862977578	22.449284976781072	30.850911714875245	23.209615445366108
49	21.641319546628228	20.403974401819607	32.24787139059172	25.70683466096045
50	19.082748495716178	30.764786374239545	27.128983504835823	23.023481625208454
51	17.696762318999614	33.20788628794794	23.774501246157197	25.320850146895246
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2455.0
1	2616.5
2	2778.0
3	6807.5
4	10837.0
5	7763.5
6	4690.0
7	5486.0
8	6282.0
9	7110.0
10	7938.0
11	8167.0
12	8396.0
13	8262.0
14	8128.0
15	7945.5
16	7763.0
17	7269.5
18	6776.0
19	6430.0
20	6084.0
21	5887.5
22	5691.0
23	5547.0
24	5403.0
25	5346.5
26	5635.0
27	5980.0
28	6172.0
29	6364.0
30	7151.0
31	7938.0
32	10849.0
33	13760.0
34	16614.0
35	19468.0
36	22309.0
37	25150.0
38	33192.5
39	41235.0
40	62780.5
41	84326.0
42	102494.5
43	120663.0
44	130841.0
45	141019.0
46	135851.0
47	130683.0
48	117647.0
49	104611.0
50	96991.5
51	89372.0
52	79096.0
53	68820.0
54	60320.0
55	51820.0
56	48061.0
57	44302.0
58	40460.0
59	36618.0
60	35304.0
61	33990.0
62	30956.0
63	27922.0
64	24491.5
65	21061.0
66	17186.0
67	13311.0
68	11064.5
69	8818.0
70	8059.5
71	7301.0
72	5799.0
73	4297.0
74	3397.0
75	2031.5
76	1566.0
77	1188.5
78	811.0
79	605.0
80	399.0
81	303.5
82	208.0
83	124.5
84	41.0
85	32.0
86	23.0
87	15.5
88	8.0
89	6.0
90	4.0
91	2.0
92	0.0
93	0.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1202898.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.477509669033466
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.69371388764867	18.362712438088764
2	7.3888972080341535	3.3216801687416413
3	2.419810428515093	1.6317393691252804
4	1.1968642983614453	1.0761011535576137
5	0.743582789250473	0.8356944667552191
6	0.5256083172565934	0.7088619619955693
7	0.3677550249746782	0.5786351990792782
8	0.3021995618932841	0.5434154859547177
9	0.24610553846033859	0.49786556583104535
>10	2.5249920487002644	13.87061005865874
>50	1.631447125418	27.233717014811493
>100	0.9536810206709326	29.460560851663793
>500	0.003816250582916897	0.6670237977153122
>1k	0.0011448751748750691	0.6466939828929704
>5k	3.816250582916897E-4	0.5646884851285879
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6583	0.5472616963366803	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	4599	0.38232668106522744	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1867	0.1552085047942552	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.313256818117579E-5	0.0	0.0	0.07947473518120406	0.0
2	8.313256818117579E-5	0.0	0.0	0.2641121691115955	0.0
3	8.313256818117579E-5	0.0	0.0	0.41433271981498015	0.0
4	8.313256818117579E-5	0.0	0.0	0.7848545761984806	0.0
5	8.313256818117579E-5	0.0	0.0	1.2903005907400296	0.0
6	8.313256818117579E-5	0.0	0.0	2.118633500097265	0.0
7	8.313256818117579E-5	0.0	0.0	2.5419445372758123	0.0
8	8.313256818117579E-5	0.0	0.0	3.739219784221106	0.0
9	8.313256818117579E-5	0.0	0.0	4.3569778983754235	0.0
10	8.313256818117579E-5	0.0	0.0	5.301114475209037	0.0
11	8.313256818117579E-5	0.0	0.0	5.971079842181132	0.0
12	8.313256818117579E-5	0.0	0.0	6.5763680711082735	8.313256818117579E-5
13	8.313256818117579E-5	0.0	0.0	6.826763366469975	8.313256818117579E-5
14	8.313256818117579E-5	0.0	0.0	6.918458589173812	8.313256818117579E-5
15	8.313256818117579E-5	0.0	0.0	7.029440567695682	8.313256818117579E-5
16	8.313256818117579E-5	0.0	0.0	7.281249116716463	8.313256818117579E-5
17	8.313256818117579E-5	0.0	0.0	7.5320600749190705	8.313256818117579E-5
18	8.313256818117579E-5	0.0	0.0	7.971581962892947	8.313256818117579E-5
19	8.313256818117579E-5	0.0	0.0	8.135602519914407	8.313256818117579E-5
20	8.313256818117579E-5	0.0	0.0	8.324562847390219	8.313256818117579E-5
21	8.313256818117579E-5	0.0	0.0	8.540707524661276	8.313256818117579E-5
22	8.313256818117579E-5	0.0	0.0	8.739560627750649	8.313256818117579E-5
23	8.313256818117579E-5	0.0	0.0	8.975158325976102	8.313256818117579E-5
24	8.313256818117579E-5	0.0	0.0	9.150069249429295	8.313256818117579E-5
25	8.313256818117579E-5	0.0	0.0	9.285907865837336	8.313256818117579E-5
26	8.313256818117579E-5	0.0	0.0	9.432470583540749	8.313256818117579E-5
27	8.313256818117579E-5	0.0	0.0	9.569306790766964	8.313256818117579E-5
28	8.313256818117579E-5	0.0	0.0	9.700074320515954	8.313256818117579E-5
29	8.313256818117579E-5	0.0	0.0	9.835912936923995	8.313256818117579E-5
30	8.313256818117579E-5	0.0	0.0	10.043162429399667	8.313256818117579E-5
31	1.6626513636235158E-4	0.0	0.0	10.22505648858008	8.313256818117579E-5
32	1.6626513636235158E-4	0.0	0.0	10.365633661374448	8.313256818117579E-5
33	1.6626513636235158E-4	0.0	0.0	10.51493975382784	8.313256818117579E-5
34	1.6626513636235158E-4	0.0	0.0	10.673806091622065	8.313256818117579E-5
35	1.6626513636235158E-4	0.0	0.0	10.889784503756761	8.313256818117579E-5
36	1.6626513636235158E-4	0.0	0.0	11.045824334232828	8.313256818117579E-5
37	1.6626513636235158E-4	0.0	0.0	11.206270190822497	8.313256818117579E-5
38	1.6626513636235158E-4	0.0	0.0	11.355908813548613	8.313256818117579E-5
39	1.6626513636235158E-4	0.0	0.0	11.5034691220702	8.313256818117579E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCGTTG	25	3.8909136E-5	45.000004	1
CGGAATA	20	7.0334174E-4	45.000004	45
CTATGCG	50	2.1827873E-11	45.000004	1
TAATACG	50	2.1827873E-11	45.000004	1
AGGTACG	25	3.8909136E-5	45.000004	1
CAAACGT	20	7.0334174E-4	45.000004	45
GTCGACA	20	7.0334174E-4	45.000004	33
CACGGTT	20	7.0334174E-4	45.000004	4
TTTACGA	20	7.0334174E-4	45.000004	39
ACGTTAT	20	7.0334174E-4	45.000004	29
GCGAAGT	20	7.0334174E-4	45.000004	39
ACGCACT	20	7.0334174E-4	45.000004	35
ACCGCGT	20	7.0334174E-4	45.000004	38
GACGTAG	90	0.0	45.000004	1
TGGCGAT	25	3.8909136E-5	45.000004	6
CGCGAGA	80	0.0	45.000004	20
TAACGGC	20	7.0334174E-4	45.000004	35
CCAGACG	25	3.8909136E-5	45.000004	21
TGCGCGA	25	3.8909136E-5	45.000004	2
GTCAACG	20	7.0334174E-4	45.000004	1
>>END_MODULE