FastQCFastQC Report
Thu 26 May 2016
SRR1546570_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546570_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1296591
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC56930.43907446527085253No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56310.4342926952292589No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15390.11869587248407555No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTATCG253.8910825E-545.00000431
TCGATAG502.1827873E-1145.0000041
TATTCGA253.8910825E-545.00000429
CGTCGAG253.8910825E-545.00000413
ATACGAT253.8910825E-545.00000414
CACAACG502.1827873E-1145.00000412
TACACCG253.8910825E-545.00000442
TACGTTC253.8910825E-545.00000420
AGTCGGT253.8910825E-545.00000416
CTAGACG207.033621E-445.01
CGAACTA207.033621E-445.043
CGTATAT207.033621E-445.027
CCCTAGT207.033621E-445.013
TCACACG207.033621E-445.043
GTTTAGT207.033621E-445.024
TAGCGAT406.8157533E-945.012
CGACCGT351.2120836E-745.08
CCGCTTC207.033621E-445.016
TACGGAT207.033621E-445.027
TCTAGCG351.2120836E-745.01