##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546570_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1296591
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.538527569603676	31.0	31.0	33.0	30.0	34.0
2	31.891575678066562	31.0	31.0	34.0	30.0	34.0
3	31.975101631894713	33.0	31.0	34.0	30.0	34.0
4	35.71995332375437	37.0	35.0	37.0	33.0	37.0
5	35.64205520476388	37.0	35.0	37.0	33.0	37.0
6	35.70056478874217	37.0	35.0	37.0	33.0	37.0
7	36.04766190726297	37.0	35.0	37.0	35.0	37.0
8	34.739334917487476	37.0	35.0	37.0	33.0	37.0
9	37.06520946080915	39.0	37.0	39.0	34.0	39.0
10	37.063285183994026	39.0	37.0	39.0	32.0	39.0
11	36.9036666149927	39.0	37.0	39.0	33.0	39.0
12	35.858799729444364	37.0	35.0	39.0	32.0	39.0
13	35.3173383125442	37.0	35.0	39.0	30.0	39.0
14	36.2429077480871	38.0	35.0	41.0	31.0	41.0
15	36.676982178651556	38.0	35.0	41.0	32.0	41.0
16	36.89737550237508	38.0	35.0	41.0	33.0	41.0
17	36.851739677353926	38.0	35.0	41.0	32.0	41.0
18	36.81907864546337	38.0	35.0	40.0	32.0	41.0
19	36.727710588767	37.0	35.0	40.0	32.0	41.0
20	36.5324084464569	36.0	35.0	40.0	32.0	41.0
21	36.38108856223744	36.0	35.0	40.0	32.0	41.0
22	36.27829670266105	36.0	35.0	40.0	32.0	41.0
23	36.26505197089907	36.0	35.0	40.0	32.0	41.0
24	36.12747813304272	35.0	35.0	40.0	31.0	41.0
25	36.02525854336487	35.0	35.0	40.0	31.0	41.0
26	35.911184020250026	35.0	35.0	40.0	31.0	41.0
27	35.810152160550246	35.0	35.0	40.0	31.0	41.0
28	35.87065466288136	36.0	35.0	40.0	31.0	41.0
29	35.8978814444956	36.0	35.0	40.0	31.0	41.0
30	35.87323141993119	36.0	35.0	40.0	31.0	41.0
31	35.67738785785186	36.0	35.0	40.0	31.0	41.0
32	35.452458022614685	35.0	34.0	40.0	30.0	41.0
33	35.29862308160399	35.0	34.0	40.0	30.0	41.0
34	35.15640938430083	35.0	34.0	40.0	29.0	41.0
35	35.040206202264244	35.0	34.0	40.0	29.0	41.0
36	34.839293963940825	35.0	34.0	40.0	27.0	41.0
37	34.71921137814469	35.0	34.0	40.0	27.0	41.0
38	34.66577895419604	35.0	34.0	40.0	27.0	41.0
39	34.65291367902446	35.0	34.0	40.0	27.0	41.0
40	34.43921251959947	35.0	34.0	40.0	25.0	41.0
41	34.43961665629331	35.0	34.0	40.0	25.0	41.0
42	34.39373325898452	35.0	34.0	40.0	25.0	41.0
43	34.29212373061359	35.0	34.0	40.0	26.0	41.0
44	34.16080938399233	35.0	34.0	40.0	25.0	41.0
45	34.02982667626106	35.0	34.0	40.0	24.0	41.0
46	34.002963926172555	35.0	34.0	40.0	24.0	41.0
47	33.956673307157	35.0	34.0	39.0	24.0	41.0
48	33.950774762434726	35.0	34.0	39.0	24.0	41.0
49	33.96197181686438	35.0	34.0	39.0	24.0	41.0
50	33.79731773550796	35.0	34.0	39.0	24.0	41.0
51	33.39938191765946	35.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	2.0
12	3.0
13	6.0
14	10.0
15	24.0
16	83.0
17	251.0
18	568.0
19	1061.0
20	1862.0
21	3148.0
22	4898.0
23	7313.0
24	11363.0
25	16140.0
26	20577.0
27	23789.0
28	24801.0
29	27379.0
30	32070.0
31	40051.0
32	50656.0
33	68419.0
34	133558.0
35	263162.0
36	83191.0
37	96642.0
38	141280.0
39	244014.0
40	268.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.51349808844886	23.822855472543	28.4523801260382	14.211266312969933
2	31.057056542888233	26.234795706587505	29.75548958769573	12.952658162828525
3	28.14395595835541	25.612625723917564	32.367107283638404	13.876311034088621
4	25.618487248484676	27.878953347663217	31.259819017716456	15.242740386135644
5	22.847451509381138	32.829396471207964	29.71646417413047	14.60668784528043
6	20.18007220472763	40.67319609653314	27.740821893719765	11.405909805019471
7	84.64450239127065	5.807691091485287	7.805622590315682	1.7421839269283836
8	82.91735790237631	7.143887316817716	6.8363115276906905	3.1024432531152843
9	81.05362446600354	5.503585941904579	10.013951970976198	3.4288376211156795
10	56.90090398591383	18.402333503780298	13.339056032318593	11.357706477987275
11	52.89995071691844	18.61681902774275	17.849884813329727	10.633345442009084
12	46.71797043169357	19.876198431116674	21.548738191148946	11.85709294604081
13	21.232292989847995	44.53655778884783	22.679935307278857	11.551213914025316
14	14.06966421948016	47.81469252833006	25.84508144819762	12.27056180399216
15	11.399199901896589	22.705849415891365	53.611894575853135	12.283056106358906
16	12.611918484703349	16.232104032806028	53.3700295621364	17.78594792035422
17	14.1201813062099	16.819876121305793	29.26227314550232	39.79766942698198
18	19.579034560628603	21.97161633853698	36.6519588675226	21.797390233311813
19	29.148590419029595	23.659504037896298	26.302511740402334	20.889393802671776
20	31.258816388514187	22.129106248616566	23.910469839756715	22.701607523112532
21	21.203525244275177	29.677207384595448	28.024180331345818	21.095087039783557
22	22.308654001146085	25.501719509081894	24.13467315444886	28.05495333532317
23	18.013930375885685	32.59092497171429	22.845677626946355	26.549467025453673
24	18.798526289323313	23.613305969268644	40.39153441601862	17.196633325389424
25	14.90007257492918	24.2106415978516	38.64780798262521	22.241477844594016
26	14.823410003617177	34.977028222469535	28.85489718808784	21.344664585825445
27	17.31795145886405	35.20701593640555	28.228099686022812	19.246932918707596
28	13.585625690753677	29.414595658924053	40.6276150304915	16.372163619830772
29	13.760545923888104	24.186501371673874	39.72648275362084	22.32646995081718
30	17.133313434999934	30.353210842894946	33.12632896572627	19.387146756378844
31	28.761806922923267	26.052085815804677	24.338206882509596	20.847900378762464
32	30.24739489939387	26.93493939106472	26.11609983410343	16.701565875437975
33	28.992180263475532	26.728012148780916	24.19868717274761	20.08112041499594
34	19.46967085225796	27.999268851935575	28.488706153289666	24.0423541425168
35	19.168882091577068	25.34800873984163	31.359464935357412	24.123644233223892
36	33.397887228894845	22.591086934893116	25.502413636991157	18.50861219922088
37	20.681772432478706	30.661635010577736	29.01500935915798	19.64158319778558
38	22.491363891928913	31.675370259395603	22.669677639286405	23.163588209389083
39	20.314038891215503	30.47738261332988	26.629137484372485	22.57944101108214
40	24.549761644188493	24.018599542955336	25.368601201149783	26.06303761170639
41	16.788948866681938	23.038645185721634	27.071374087896643	33.10103185969978
42	22.873211367347142	25.104986846276116	24.137603916732417	27.88419786964432
43	23.235083384043236	25.879710718337552	25.393821181853028	25.49138471576619
44	20.999683014921438	30.840565760521244	27.277838578240942	20.881912646316376
45	16.90255446783141	37.331510090691665	21.984419142196728	23.781516299280188
46	23.310820451476218	32.18254638509754	25.960538057105133	18.546095106321115
47	23.451574166410225	25.506269903153733	26.77513572128759	24.267020209148452
48	24.970171781232477	21.755973934725752	30.38691460915586	22.886939674885912
49	22.62795283940734	20.24339209511712	32.42124926056096	24.707405804914583
50	19.256265082821027	31.836176558375	26.17417520251182	22.733383156292152
51	18.377576275016562	33.83464793446815	22.954270082084484	24.8335057084308
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1955.0
1	2243.0
2	2531.0
3	6091.5
4	9652.0
5	7377.5
6	5103.0
7	5738.0
8	6373.0
9	7223.5
10	8074.0
11	8414.5
12	8755.0
13	8691.5
14	8628.0
15	8512.0
16	8396.0
17	7818.0
18	7240.0
19	6763.0
20	6286.0
21	5977.0
22	5668.0
23	5477.5
24	5287.0
25	5597.0
26	6076.0
27	6245.0
28	6725.5
29	7206.0
30	8836.0
31	10466.0
32	12325.0
33	14184.0
34	17944.5
35	21705.0
36	22790.5
37	23876.0
38	31934.5
39	39993.0
40	62341.0
41	84689.0
42	108883.0
43	133077.0
44	134066.5
45	135056.0
46	132820.5
47	130585.0
48	125862.5
49	121140.0
50	120120.0
51	119100.0
52	110484.0
53	101868.0
54	85237.5
55	68607.0
56	58345.0
57	48083.0
58	42994.0
59	37905.0
60	33744.5
61	29584.0
62	26601.0
63	23618.0
64	20450.0
65	17282.0
66	14449.0
67	11616.0
68	9827.0
69	8038.0
70	6694.5
71	5351.0
72	4345.0
73	3339.0
74	2806.0
75	1637.0
76	1001.0
77	748.0
78	495.0
79	370.0
80	245.0
81	138.0
82	31.0
83	31.0
84	31.0
85	36.5
86	42.0
87	22.0
88	2.0
89	2.5
90	3.0
91	1.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1296591.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.304736079395855
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.18834449595377	17.510009882887623
2	7.359056352553367	3.1356550676910198
3	2.425840329545529	1.5504566397516648
4	1.1582933344122752	0.9870853516870771
5	0.7023417672368519	0.7481602994258804
6	0.4859011302346197	0.6211197204197237
7	0.34679501624528697	0.5171863406328947
8	0.278478428460769	0.4746327537729282
9	0.22042062008826385	0.42264028236935175
>10	2.1963301742124814	11.041154787087718
>50	1.3120052377721938	21.05669283548321
>100	1.3202192704319526	40.25284058612767
>500	0.004480382139474951	0.578526900104945
>1k	7.467303565791587E-4	0.2030770213072906
>5k	7.467303565791587E-4	0.9007615312509827
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	5693	0.43907446527085253	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5631	0.4342926952292589	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1539	0.11869587248407555	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.08344959975813498	0.0
2	0.0	0.0	0.0	0.2597580887110893	0.0
3	0.0	0.0	0.0	0.3941875271384731	0.0
4	0.0	0.0	0.0	0.6878036327569758	0.0
5	0.0	0.0	0.0	1.0883925617253243	0.0
6	0.0	0.0	0.0	1.750976213779056	0.0
7	0.0	0.0	0.0	2.110765846747355	0.0
8	0.0	0.0	0.0	3.188206612570965	0.0
9	0.0	0.0	0.0	3.7538437332975474	0.0
10	0.0	0.0	0.0	4.635540428708822	0.0
11	0.0	0.0	0.0	5.222541263976073	0.0
12	0.0	0.0	0.0	5.756865503462541	0.0
13	0.0	0.0	0.0	5.985619212226523	0.0
14	0.0	0.0	0.0	6.069762939893922	0.0
15	0.0	0.0	0.0	6.166940847190825	0.0
16	0.0	0.0	0.0	6.38659376781113	0.0
17	0.0	0.0	0.0	6.611028458473027	0.0
18	0.0	0.0	0.0	7.024574441747629	0.0
19	0.0	0.0	0.0	7.165096780711882	0.0
20	0.0	0.0	0.0	7.351508687010784	0.0
21	0.0	0.0	0.0	7.552111652787965	0.0
22	0.0	0.0	0.0	7.734667292924292	0.0
23	7.712532325151108E-5	0.0	0.0	7.964192254920788	0.0
24	7.712532325151108E-5	0.0	0.0	8.121219413060865	0.0
25	7.712532325151108E-5	0.0	0.0	8.243925802354019	0.0
26	7.712532325151108E-5	0.0	0.0	8.368328948758707	0.0
27	7.712532325151108E-5	0.0	0.0	8.496896862618975	0.0
28	7.712532325151108E-5	0.0	0.0	8.607340325515139	0.0
29	7.712532325151108E-5	0.0	0.0	8.731743471919827	0.0
30	7.712532325151108E-5	0.0	0.0	8.928875798150688	0.0
31	7.712532325151108E-5	0.0	0.0	9.09222723279739	0.0
32	7.712532325151108E-5	0.0	0.0	9.224574287496983	0.0
33	7.712532325151108E-5	0.0	0.0	9.35761547010584	0.0
34	7.712532325151108E-5	0.0	0.0	9.493818790968009	0.0
35	7.712532325151108E-5	0.0	0.0	9.678842441448383	0.0
36	7.712532325151108E-5	0.0	0.0	9.829005445819075	0.0
37	7.712532325151108E-5	0.0	0.0	9.974849432087682	0.0
38	7.712532325151108E-5	0.0	0.0	10.113983515233409	0.0
39	7.712532325151108E-5	0.0	0.0	10.249261332216559	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTATCG	25	3.8910825E-5	45.000004	31
TCGATAG	50	2.1827873E-11	45.000004	1
TATTCGA	25	3.8910825E-5	45.000004	29
CGTCGAG	25	3.8910825E-5	45.000004	13
ATACGAT	25	3.8910825E-5	45.000004	14
CACAACG	50	2.1827873E-11	45.000004	12
TACACCG	25	3.8910825E-5	45.000004	42
TACGTTC	25	3.8910825E-5	45.000004	20
AGTCGGT	25	3.8910825E-5	45.000004	16
CTAGACG	20	7.033621E-4	45.0	1
CGAACTA	20	7.033621E-4	45.0	43
CGTATAT	20	7.033621E-4	45.0	27
CCCTAGT	20	7.033621E-4	45.0	13
TCACACG	20	7.033621E-4	45.0	43
GTTTAGT	20	7.033621E-4	45.0	24
TAGCGAT	40	6.8157533E-9	45.0	12
CGACCGT	35	1.2120836E-7	45.0	8
CCGCTTC	20	7.033621E-4	45.0	16
TACGGAT	20	7.033621E-4	45.0	27
TCTAGCG	35	1.2120836E-7	45.0	1
>>END_MODULE