FastQCFastQC Report
Thu 26 May 2016
SRR1546569_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546569_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1801954
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCG224591.246369219192055No Hit
GAATCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTC180641.0024673215853457No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGC140830.7815404832753777No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117390.6514594712184661No Hit
GAACTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCT54600.30300440521789124No Hit
GAATGACTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCT53450.2966224443021298No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTC52660.29223831462956323No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC52370.2906289505725451No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGATAAGTGT42320.23485616170002122No Hit
GCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGC41480.2301945554658998TruSeq Adapter, Index 15 (95% over 22bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGATAAGTGTCGTA26710.14822797918259845No Hit
CTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGCT23660.13130190892775287TruSeq Adapter, Index 21 (95% over 22bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22490.12480895738736948No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCAAT207.0343557E-445.041
TTCGTAA207.0343557E-445.021
CGTTCCA207.0343557E-445.022
TCTAGCG406.8193913E-945.01
TATCTCG551.8189894E-1245.01
ACTACGC207.0343557E-445.044
GTATATC207.0343557E-445.021
AACTACG253.8916907E-545.026
TAAGCCA207.0343557E-445.037
ATGCACG351.2124474E-745.01
TCGAATC253.8916907E-545.020
TACGTAG253.8916907E-545.01
GTACGCA302.1661908E-644.99999637
CGACGGT1650.043.63636428
GATGAAT72850.043.517520
CGATGAA70500.043.05319219
TGAATGA73100.042.87619422
CGTCGAA7000.042.7542
ACGTAAG1000.042.751
GTGTACG850.042.352941