##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546569_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1801954
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.268399748273264	31.0	31.0	33.0	30.0	34.0
2	31.606938911870113	31.0	31.0	34.0	30.0	34.0
3	31.708591340289487	31.0	31.0	34.0	30.0	34.0
4	35.49392825788006	37.0	35.0	37.0	33.0	37.0
5	35.34051035709014	37.0	35.0	37.0	33.0	37.0
6	35.41998630375692	37.0	35.0	37.0	33.0	37.0
7	35.73772637925274	37.0	35.0	37.0	35.0	37.0
8	34.371661540749656	37.0	35.0	37.0	32.0	37.0
9	36.59586149257972	39.0	37.0	39.0	32.0	39.0
10	36.626387799022616	39.0	35.0	39.0	32.0	39.0
11	36.51203193866214	39.0	35.0	39.0	32.0	39.0
12	35.825513858844346	37.0	35.0	39.0	31.0	39.0
13	35.36316243366923	37.0	35.0	39.0	30.0	39.0
14	36.39547513421541	38.0	35.0	41.0	31.0	41.0
15	36.638246592310345	38.0	35.0	41.0	31.0	41.0
16	36.90517959947923	38.0	35.0	41.0	32.0	41.0
17	36.608650387301786	38.0	35.0	41.0	31.0	41.0
18	36.64241484521803	38.0	35.0	40.0	31.0	41.0
19	36.577384883298905	37.0	35.0	40.0	31.0	41.0
20	36.00371430125297	37.0	35.0	40.0	30.0	41.0
21	36.19483238750823	37.0	35.0	40.0	31.0	41.0
22	36.03703202190511	37.0	35.0	40.0	30.0	41.0
23	35.72353012341048	37.0	34.0	40.0	30.0	41.0
24	35.78477530502998	36.0	34.0	40.0	30.0	41.0
25	35.94989494737379	36.0	35.0	40.0	30.0	41.0
26	35.59949643553609	36.0	34.0	40.0	30.0	41.0
27	35.273736177505086	36.0	34.0	40.0	29.0	41.0
28	35.463440243202655	36.0	34.0	40.0	29.0	41.0
29	35.36729794434264	36.0	34.0	40.0	29.0	41.0
30	35.49468354908061	36.0	34.0	40.0	29.0	41.0
31	35.37768500194789	36.0	34.0	40.0	29.0	41.0
32	34.868951149696386	36.0	34.0	40.0	26.0	41.0
33	34.634330843073684	36.0	34.0	40.0	24.0	41.0
34	34.33529490763915	36.0	34.0	40.0	23.0	41.0
35	34.073670582045935	36.0	33.0	40.0	22.0	41.0
36	33.90070112777574	36.0	33.0	40.0	21.0	41.0
37	33.69190001520572	36.0	33.0	40.0	19.0	41.0
38	33.669813435859076	35.0	33.0	40.0	20.0	41.0
39	33.62619911496076	36.0	33.0	40.0	18.0	41.0
40	33.4696584929471	35.0	33.0	40.0	18.0	41.0
41	33.47235612007854	35.0	33.0	40.0	18.0	41.0
42	33.34234669697451	35.0	33.0	40.0	18.0	41.0
43	33.184561870058836	35.0	33.0	40.0	17.0	41.0
44	33.231598031914245	35.0	33.0	40.0	18.0	41.0
45	33.20749364301197	35.0	33.0	40.0	18.0	41.0
46	33.20012053581834	35.0	33.0	40.0	18.0	41.0
47	33.02723099479787	35.0	32.0	40.0	18.0	41.0
48	33.029448587477816	35.0	32.0	39.0	18.0	41.0
49	32.998678656613876	35.0	32.0	39.0	18.0	41.0
50	32.768566789163316	35.0	32.0	39.0	17.0	41.0
51	32.471989851017284	35.0	32.0	39.0	17.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	2.0
13	14.0
14	22.0
15	52.0
16	191.0
17	558.0
18	1504.0
19	3361.0
20	7759.0
21	14702.0
22	20219.0
23	23591.0
24	26819.0
25	31812.0
26	36248.0
27	38440.0
28	38651.0
29	41613.0
30	48420.0
31	58715.0
32	73403.0
33	94217.0
34	159085.0
35	277236.0
36	121840.0
37	143475.0
38	201552.0
39	338138.0
40	313.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.59684875418573	22.00250394849147	24.50828378526866	13.892363512054137
2	27.85426265043392	33.918956865713554	26.165817773372684	12.060962710479846
3	25.74138962481839	31.955477220839157	29.816243921875916	12.486889232466533
4	22.54580305601586	25.483946871007806	37.417769820983224	14.552480251993114
5	28.91955066555528	29.313289906401607	27.285269213309554	14.48189021473356
6	18.350745912492773	44.80175409583153	26.38835397573967	10.459146015936033
7	75.91725426953185	4.856561266269838	16.86741170973288	2.35877275446543
8	72.65440738220843	13.746410840676287	9.021317969271134	4.577863807844151
9	70.68543370141525	5.615015699623853	11.576488634005086	12.12306196495582
10	50.627041533801645	19.145105812911982	16.263567216477224	13.964285436809154
11	45.236948334974144	19.340060845060417	22.158057308899117	13.264933511066321
12	36.44049737118706	18.043912330725423	25.2987590138261	20.21683128426142
13	25.000416214842332	34.57230317755059	27.831121105200246	12.596159502406831
14	14.927573067902955	44.15295839960399	26.542297972090296	14.377170560402764
15	12.327673181446364	22.133694866794603	47.873808099429844	17.664823852329196
16	13.73275899384779	18.525278669710772	43.00564831288701	24.73631402355443
17	13.55750479756975	23.062852880817157	30.12096868177545	33.258673639837646
18	18.2097323239106	21.71736903383771	34.637843141389844	25.435055500861843
19	24.484254315038008	24.9577403196752	25.441381966465293	25.116623398821503
20	31.07726390351807	22.07475884512035	26.34778690244035	20.500190348921226
21	19.959000063264657	32.51764473454927	27.472898864232935	20.050456337953133
22	19.64850379088478	22.797640783283036	30.157262616026827	27.396592809805355
23	21.277180216587105	30.15082515979875	24.44757191360046	24.124422710013686
24	19.03605752422093	26.758729690103078	34.93918268723841	19.266030098437582
25	16.171444998041014	30.12390993332793	32.54600283914017	21.158642229490876
26	15.745795952615882	29.700425205082926	32.57896705465289	21.974811787648296
27	21.955776895525638	30.8745395276461	26.827987839867166	20.3416957369611
28	15.114758756327854	31.024487861510337	36.59371992847764	17.267033453684167
29	17.643735633651026	22.950308387450512	37.36954439458499	22.036411584313473
30	17.69257150848468	34.08344497140327	30.31542425611309	17.908559263998967
31	25.991839969277798	25.744330876370874	23.775745662763867	24.488083491587464
32	31.555633495638624	27.72551352587247	24.63553453639771	16.083318442091198
33	28.459605517121965	28.089451784007807	22.818729001961206	20.632213696909023
34	21.95261366272391	26.352947966485267	27.01794829390761	24.676490076883205
35	22.851859703410852	27.018669732967656	28.599176227584056	21.530294336037432
36	29.063283524440692	26.763280305712577	26.954572647248487	17.218863522598244
37	20.85197513366046	31.213338409304566	29.077934286890788	18.85675217014419
38	20.228651785783654	30.77675678735417	24.54740798044789	24.447183446414282
39	23.35087355171109	30.21608764707645	25.864589218148744	20.568449583063718
40	22.02020695311867	25.293209482595003	27.749376510166186	24.93720705412014
41	19.986525738170897	22.475823467191727	26.43347166464849	31.10417912998889
42	23.03682557934331	23.887846193632022	27.240096029088424	25.835232197936243
43	25.46596638981905	24.700630537738476	25.061905020882886	24.771498051559586
44	21.877195533293303	27.52251167343894	28.030959724832044	22.56933306843571
45	17.52713998248568	31.562181942491318	26.11859126259605	24.792086812426955
46	25.5820625831736	30.288398039017643	25.254640240538883	18.874899137269875
47	21.09188136878078	27.539215762444545	29.177770353738218	22.191132515036454
48	24.167653558303932	23.090212069786464	29.252078576922607	23.490055794986997
49	21.754606388398372	20.450411053778286	34.1903844382265	23.604598119596837
50	21.541781865685806	27.487438636058414	28.382134061135854	22.588645437119926
51	20.096406456546614	29.239869608214192	24.604901124002055	26.058822811237135
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2923.0
1	3434.5
2	3946.0
3	11330.0
4	18714.0
5	13161.0
6	7608.0
7	8357.5
8	9107.0
9	10011.0
10	10915.0
11	11518.5
12	12122.0
13	11985.5
14	11849.0
15	11238.5
16	10628.0
17	10243.0
18	9858.0
19	9851.5
20	9845.0
21	8973.0
22	8101.0
23	8029.0
24	7957.0
25	8088.0
26	8981.5
27	9744.0
28	11478.0
29	13212.0
30	15543.0
31	17874.0
32	20894.0
33	23914.0
34	27307.5
35	30701.0
36	30652.5
37	30604.0
38	40239.0
39	49874.0
40	75698.5
41	101523.0
42	129969.5
43	158416.0
44	208764.0
45	259112.0
46	224682.5
47	190253.0
48	171975.0
49	153697.0
50	141477.0
51	129257.0
52	119232.0
53	109207.0
54	100710.0
55	92213.0
56	86005.5
57	79798.0
58	71203.0
59	62608.0
60	54543.0
61	46478.0
62	40127.5
63	33777.0
64	30120.0
65	26463.0
66	21687.5
67	16912.0
68	14201.0
69	11490.0
70	10032.0
71	8574.0
72	7173.5
73	5773.0
74	4617.0
75	2847.0
76	2233.0
77	1695.5
78	1158.0
79	1140.5
80	1123.0
81	737.5
82	352.0
83	277.5
84	203.0
85	163.5
86	124.0
87	69.5
88	15.0
89	15.0
90	15.0
91	9.5
92	4.0
93	2.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1801954.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.234907993205518
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.91950309479031	19.033921172565677
2	7.587100607501847	3.525711691009982
3	2.5217088981743503	1.7577502270418606
4	1.2669699720956606	1.1775172292718734
5	0.7743612616418054	0.8996106333874947
6	0.49418838169292556	0.6889452947967752
7	0.3752956047701379	0.6103971192962003
8	0.3011835575279032	0.5598377798581721
9	0.23981038765456467	0.5014775063671882
>10	2.402273360758763	13.248618849770152
>50	1.2412818147465863	21.17716441611743
>100	0.8635264287565095	29.00056598321567
>500	0.007136582053275074	1.0939974108709414
>1k	0.0036913355447974524	1.712612264172186
>5k	9.843561452793205E-4	1.2183607814162971
>10k+	9.843561452793205E-4	3.7935116408421363
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCG	22459	1.246369219192055	No Hit
GAATCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTC	18064	1.0024673215853457	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGC	14083	0.7815404832753777	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11739	0.6514594712184661	No Hit
GAACTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCT	5460	0.30300440521789124	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCT	5345	0.2966224443021298	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTC	5266	0.29223831462956323	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	5237	0.2906289505725451	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGATAAGTGT	4232	0.23485616170002122	No Hit
GCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGC	4148	0.2301945554658998	TruSeq Adapter, Index 15 (95% over 22bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGATAAGTGTCGTA	2671	0.14822797918259845	No Hit
CTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGCT	2366	0.13130190892775287	TruSeq Adapter, Index 21 (95% over 22bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2249	0.12480895738736948	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.14090259795755053	0.0
2	0.0	0.0	0.0	0.5538432168634715	0.0
3	0.0	0.0	0.0	0.7727722239302446	0.0
4	0.0	0.0	0.0	1.4909925558588066	0.0
5	0.0	0.0	0.0	3.1161172815732257	0.0
6	0.0	0.0	0.0	4.0588161517996575	0.0
7	0.0	0.0	0.0	4.791187788367517	0.0
8	0.0	0.0	0.0	6.209536980411265	0.0
9	0.0	0.0	0.0	6.726697795837185	0.0
10	0.0	0.0	0.0	8.9219813602345	0.0
11	0.0	0.0	0.0	9.564117618984724	0.0
12	0.0	0.0	0.0	11.038794553024106	0.0
13	0.0	0.0	0.0	11.371322464391433	0.0
14	0.0	0.0	0.0	11.525599432615927	0.0
15	0.0	0.0	0.0	11.833154453443317	0.0
16	0.0	0.0	0.0	12.210966539656395	0.0
17	0.0	0.0	0.0	12.539609779161955	0.0
18	0.0	0.0	0.0	13.068036142987001	0.0
19	0.0	0.0	0.0	13.538025942948599	0.0
20	5.5495312310969095E-5	0.0	0.0	13.78486909210779	0.0
21	5.5495312310969095E-5	0.0	0.0	14.031878727203914	0.0
22	5.5495312310969095E-5	0.0	0.0	14.286935182585127	0.0
23	5.5495312310969095E-5	0.0	0.0	14.551481336371516	0.0
24	5.5495312310969095E-5	0.0	0.0	14.745048985712177	0.0
25	5.5495312310969095E-5	5.5495312310969095E-5	0.0	14.89410939457944	0.0
26	5.5495312310969095E-5	5.5495312310969095E-5	0.0	15.04755393311927	0.0
27	5.5495312310969095E-5	5.5495312310969095E-5	0.0	15.238957265279803	0.0
28	5.5495312310969095E-5	5.5495312310969095E-5	0.0	15.39517656943518	0.0
29	5.5495312310969095E-5	5.5495312310969095E-5	0.0	15.557333872007831	0.0
30	5.5495312310969095E-5	5.5495312310969095E-5	0.0	15.792800482143273	0.0
31	5.5495312310969095E-5	5.5495312310969095E-5	0.0	15.982039497123678	0.0
32	5.5495312310969095E-5	5.5495312310969095E-5	0.0	16.147415527810367	0.0
33	5.5495312310969095E-5	5.5495312310969095E-5	0.0	16.315566324112602	0.0
34	5.5495312310969095E-5	5.5495312310969095E-5	0.0	16.487657287588917	0.0
35	5.5495312310969095E-5	5.5495312310969095E-5	0.0	16.691436074394797	0.0
36	5.5495312310969095E-5	5.5495312310969095E-5	0.0	16.868299634729855	0.0
37	5.5495312310969095E-5	5.5495312310969095E-5	0.0	17.035451515410493	0.0
38	5.5495312310969095E-5	5.5495312310969095E-5	0.0	17.19377964143369	0.0
39	5.5495312310969095E-5	5.5495312310969095E-5	0.0	17.351386328396842	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGCCAAT	20	7.0343557E-4	45.0	41
TTCGTAA	20	7.0343557E-4	45.0	21
CGTTCCA	20	7.0343557E-4	45.0	22
TCTAGCG	40	6.8193913E-9	45.0	1
TATCTCG	55	1.8189894E-12	45.0	1
ACTACGC	20	7.0343557E-4	45.0	44
GTATATC	20	7.0343557E-4	45.0	21
AACTACG	25	3.8916907E-5	45.0	26
TAAGCCA	20	7.0343557E-4	45.0	37
ATGCACG	35	1.2124474E-7	45.0	1
TCGAATC	25	3.8916907E-5	45.0	20
TACGTAG	25	3.8916907E-5	45.0	1
GTACGCA	30	2.1661908E-6	44.999996	37
CGACGGT	165	0.0	43.636364	28
GATGAAT	7285	0.0	43.5175	20
CGATGAA	7050	0.0	43.053192	19
TGAATGA	7310	0.0	42.876194	22
CGTCGAA	700	0.0	42.75	42
ACGTAAG	100	0.0	42.75	1
GTGTACG	85	0.0	42.35294	1
>>END_MODULE