FastQCFastQC Report
Thu 26 May 2016
SRR1546568_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546568_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences404585
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43941.0860511388212613No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG207.0278614E-445.01
TCGATAG253.886309E-545.01
TGATTCG700.045.015
GCGATAC453.8380676E-1045.09
CGTAAGG406.7957444E-945.02
AGCTAAG207.0278614E-445.01
TACGCGG302.1620017E-644.9999962
CGTTTTT28750.042.4173931
GGCCGAT1100.040.9090928
AACGGGC501.0786607E-940.54
TAAGGGA2400.039.3749964
AGGGCGC1150.039.1304326
CGTTCGG1450.038.79310245
TATGGGC2250.038.04
GGCGATA2200.037.8409088
GGGCGAT9100.037.582427
ATTAGCG301.1386149E-437.4999961
TGAACGG301.1386149E-437.4999962
CTACGGG601.546141E-1037.4999963
GCGATCG301.1386149E-437.4999969
Produced by FastQC (version 0.11.5)