FastQCFastQC Report
Thu 26 May 2016
SRR1546567_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546567_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences144656
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9310.6435958411680124No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC1800.12443313792722044No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACGAC253.8738595E-545.00000412
GCGCAAT253.8738595E-545.00000419
TAGGGTA502.1827873E-1145.0000045
CGCAATC253.8738595E-545.00000420
AGTAGCG302.1523192E-645.0000041
CAATCGG253.8738595E-545.00000422
ACGACGC253.8738595E-545.00000414
GCAACGA207.012833E-445.011
TCGTAAG207.012833E-445.01
GTAACGG207.012833E-445.02
TGCGTAG207.012833E-445.01
TAGTAAG207.012833E-445.01
GTTGATC1150.043.04347616
TTGATCC1100.042.95454417
GTAGCAT1100.042.95454429
ATCCTGC1050.042.85714320
AAGGGAC1000.042.7500045
CGTTTTT6350.042.5196841
TGATCCT1150.041.08695618
TAGCGGG1150.041.0869563
Produced by FastQC (version 0.11.5)