##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546567_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	144656
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.42375013825904	31.0	31.0	33.0	30.0	34.0
2	31.805905043689858	31.0	31.0	34.0	30.0	34.0
3	31.911265346753677	31.0	31.0	34.0	30.0	34.0
4	35.67357040150426	37.0	35.0	37.0	33.0	37.0
5	35.613261807322196	37.0	35.0	37.0	33.0	37.0
6	35.64816530251078	37.0	35.0	37.0	33.0	37.0
7	35.99550658113041	37.0	35.0	37.0	35.0	37.0
8	34.65000414777126	37.0	35.0	37.0	33.0	37.0
9	36.95557045680788	39.0	37.0	39.0	33.0	39.0
10	36.867679183718614	39.0	37.0	39.0	32.0	39.0
11	36.80583591416879	39.0	37.0	39.0	32.0	39.0
12	36.44773116911846	39.0	35.0	39.0	32.0	39.0
13	36.30802040703462	39.0	35.0	39.0	32.0	39.0
14	37.39740487777901	40.0	35.0	41.0	32.0	41.0
15	37.566302123658886	40.0	35.0	41.0	33.0	41.0
16	37.626182114810305	40.0	35.0	41.0	33.0	41.0
17	37.51009291007632	39.0	35.0	41.0	32.0	41.0
18	37.42920445747152	39.0	35.0	41.0	32.0	41.0
19	37.353659716845485	39.0	35.0	41.0	32.0	41.0
20	37.20003318217012	39.0	35.0	41.0	32.0	41.0
21	37.09241234376728	39.0	35.0	41.0	32.0	41.0
22	36.98968587545625	39.0	35.0	41.0	32.0	41.0
23	36.938661375953984	39.0	35.0	41.0	32.0	41.0
24	36.80327120893706	38.0	35.0	41.0	31.0	41.0
25	36.6891660214578	38.0	35.0	41.0	31.0	41.0
26	36.585734432031856	38.0	35.0	41.0	31.0	41.0
27	36.46499281052981	38.0	35.0	40.0	31.0	41.0
28	36.48483298307709	38.0	35.0	40.0	31.0	41.0
29	36.42975749364008	38.0	35.0	40.0	31.0	41.0
30	36.35871999778786	38.0	35.0	40.0	31.0	41.0
31	36.23930566309036	38.0	35.0	40.0	30.0	41.0
32	36.096435681893595	38.0	35.0	41.0	30.0	41.0
33	35.925201858201525	38.0	35.0	41.0	30.0	41.0
34	35.73022204402168	38.0	35.0	41.0	29.0	41.0
35	35.58702024112377	38.0	35.0	41.0	28.0	41.0
36	35.46055469527707	38.0	35.0	40.0	27.0	41.0
37	35.414673432142465	38.0	35.0	40.0	27.0	41.0
38	35.316841333923236	38.0	34.0	40.0	27.0	41.0
39	35.24545127751355	38.0	34.0	40.0	26.0	41.0
40	35.097362017475945	38.0	34.0	40.0	25.0	41.0
41	35.00163145669727	37.0	34.0	40.0	25.0	41.0
42	34.95416021457803	37.0	34.0	40.0	25.0	41.0
43	34.8405734985068	37.0	34.0	40.0	24.0	41.0
44	34.708328724698596	37.0	34.0	40.0	24.0	41.0
45	34.608084006194005	37.0	34.0	40.0	24.0	41.0
46	34.53552566087822	36.0	34.0	40.0	24.0	41.0
47	34.47932336024776	36.0	34.0	40.0	23.0	41.0
48	34.39826208384029	36.0	34.0	40.0	23.0	41.0
49	34.31677911735427	36.0	34.0	40.0	24.0	41.0
50	34.206628138480255	36.0	33.0	40.0	24.0	41.0
51	33.842177303395644	35.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	2.0
13	1.0
14	4.0
15	4.0
16	15.0
17	36.0
18	58.0
19	126.0
20	240.0
21	380.0
22	591.0
23	845.0
24	1133.0
25	1651.0
26	2257.0
27	2700.0
28	2863.0
29	3107.0
30	3402.0
31	4136.0
32	5132.0
33	6454.0
34	10924.0
35	16788.0
36	11265.0
37	14488.0
38	20864.0
39	35165.0
40	23.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.19646609888287	25.363621280831765	28.429515540316334	16.01039707996903
2	33.24093020683553	25.78323747373078	27.077342108173873	13.898490211259817
3	28.860883751797367	25.19563654463002	31.72699369538768	14.216486008184937
4	25.856514766065704	27.490045348965825	30.745354496184053	15.908085388784427
5	23.137650702355934	31.10828448180511	30.21374847915054	15.54031633668842
6	21.3700088485787	39.981749806437335	27.680842827120895	10.967398517863067
7	85.60861630350625	4.286721601592744	8.159357371972128	1.9453047229288793
8	83.61008184935295	6.088236920694613	7.8185488330936845	2.4831323968587546
9	80.91610441322862	5.190935737197213	10.298224753898905	3.594735095675257
10	43.642157947129746	23.873880101758655	17.710292003097003	14.7736699480146
11	35.30029863953102	24.21607123105851	24.406177414002876	16.077452715407585
12	31.912260811857095	21.85875456254839	28.19654905430815	18.032435571286364
13	24.272757438336466	28.98324300409247	29.37797256940604	17.366026988165025
14	19.906536887512445	32.26758655016038	30.522757438336466	17.303119123990708
15	18.832955425284815	23.35540869372857	39.93750691295211	17.87412896803451
16	21.537993584780445	21.19787634111271	37.65139365114478	19.61273642296206
17	21.18197655126645	21.60919699148324	30.256608782214357	26.95221767503595
18	23.857289016701692	23.27798363012941	32.383032850348414	20.481694502820485
19	26.903135715075766	24.61978763411127	27.72577701581683	20.75129963499613
20	28.18410574051543	23.93333148987944	26.55403163366884	21.32853113593629
21	25.362929985621058	25.43344209711315	29.25768720274306	19.94594071452273
22	24.292113704236257	23.38720827342108	28.232496405264907	24.088181617077755
23	22.803063820373854	26.782850348412783	26.951526379825243	23.46255945138812
24	22.420086273642294	24.73039486782436	32.681672381373744	20.167846477159607
25	22.129050989934743	24.618405043689854	31.08892821590532	22.163615750470083
26	20.378691516425175	28.22005309147218	28.51523614644398	22.886019245658666
27	21.02574383364672	27.360081849352945	29.465075765955095	22.14909855104524
28	20.04894370091804	26.55403163366884	32.86693949784316	20.530085167569958
29	21.04510009954651	24.9488441544077	32.2703517310032	21.735704015042582
30	22.311552925561333	25.00829554252848	31.623299413781663	21.056852118128525
31	26.545044795929655	25.12028536666298	26.95843933193231	21.376230505475057
32	26.09501161375954	25.539901559561994	27.73752903439885	20.627557792279617
33	25.92011392545072	25.119594071452273	26.887927220440215	22.072364782656788
34	22.740155956199533	26.317608671607122	29.502405707333256	21.43982966486008
35	22.239658223647826	25.358090919146115	29.69942484238469	22.70282601482137
36	26.227048999004538	25.9069793164473	27.207305607786747	20.65866607676142
37	22.341278619621725	27.255696272536223	28.97218228072116	21.430842827120895
38	24.0826512553921	28.428824245105627	25.421690078531135	22.066834420971134
39	22.744995022674484	26.93908306603252	26.95636544630019	23.35955646499281
40	23.55242782878	25.29656564539321	27.762415661984292	23.388590863842495
41	20.7077480367216	24.82164583563765	28.81733215352284	25.65327397411791
42	24.127585444088044	26.2332706559009	25.9069793164473	23.732164583563765
43	23.292500829554253	25.535753788297754	27.86403605795819	23.3077093241898
44	22.60327950447959	27.526683995133283	27.089094126755892	22.780942373631234
45	21.161928990155957	28.768941488773365	26.98194336909634	23.08718615197434
46	23.65543081517531	27.228735759318663	27.1305718393983	21.985261586107733
47	23.329830770932418	26.737916159716846	27.43197655126645	22.500276518084284
48	24.06398628470302	25.382977546731556	28.473067138590864	22.07996902997456
49	22.975887623050546	23.981722154628912	29.391798473620174	23.650591748700364
50	21.848385134387787	27.731998672713193	28.04722928879549	22.37238690410353
51	21.384526048003536	28.67146886406371	26.815341223316004	23.128663864616748
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	168.0
1	233.5
2	299.0
3	990.0
4	1681.0
5	1212.5
6	744.0
7	750.0
8	756.0
9	787.0
10	818.0
11	834.0
12	850.0
13	854.5
14	859.0
15	811.0
16	763.0
17	720.5
18	678.0
19	682.5
20	687.0
21	702.0
22	717.0
23	754.5
24	792.0
25	831.5
26	1047.5
27	1224.0
28	1351.0
29	1478.0
30	1748.5
31	2019.0
32	2287.0
33	2555.0
34	2840.0
35	3125.0
36	3476.0
37	3827.0
38	4345.5
39	4864.0
40	6153.5
41	7443.0
42	8483.5
43	9524.0
44	9907.0
45	10290.0
46	10353.5
47	10417.0
48	10962.0
49	11507.0
50	11293.5
51	11080.0
52	10584.0
53	10088.0
54	9303.0
55	8518.0
56	7921.0
57	7324.0
58	6950.5
59	6577.0
60	6027.5
61	5478.0
62	5011.0
63	4544.0
64	4237.0
65	3930.0
66	3322.0
67	2714.0
68	2312.5
69	1911.0
70	1596.0
71	1281.0
72	1094.5
73	908.0
74	757.5
75	491.5
76	376.0
77	274.0
78	172.0
79	125.0
80	78.0
81	79.5
82	81.0
83	49.5
84	18.0
85	12.5
86	7.0
87	6.0
88	5.0
89	4.0
90	3.0
91	1.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	144656.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.04700807432806
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.48853527819216	33.775992699922575
2	10.949499972374165	10.959794270545293
3	6.847063373667053	10.280251078420529
4	4.804132825017956	9.617298971352726
5	3.458754627327477	8.655016038048888
6	2.2694624012376376	6.81478818714744
7	1.4973202939388917	5.245548058843048
8	0.9489474556605337	3.7993584780444642
9	0.6215813028344107	2.79974560336246
>10	1.1050334272611746	7.052593739630572
>50	0.005525167136305873	0.154158831987612
>100	0.0027625835681529365	0.20185820152637982
>500	0.0013812917840764682	0.6435958411680124
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	931	0.6435958411680124	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	180	0.12443313792722044	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.03179957969251189	0.0
2	0.0	0.0	0.0	0.09263355823470855	0.0
3	0.0	0.0	0.0	0.16660214578033403	0.0
4	0.0	0.0	0.0	0.2834310363897799	0.0
5	0.0	0.0	0.0	0.4832153522840394	0.0
6	0.0	0.0	0.0	0.884166574493972	0.0
7	0.0	0.0	0.0	1.1005419754451942	0.0
8	0.0	0.0	0.0	1.6335305829001217	0.0
9	0.0	0.0	0.0	1.9425395420860525	0.0
10	0.0	0.0	0.0	2.358699258931534	0.0
11	0.0	0.0	0.0	2.652499723481916	0.0
12	0.0	0.0	0.0	2.9151919035504923	0.0
13	0.0	0.0	0.0	3.0306382037385244	0.0
14	0.0	0.0	0.0	3.0797201636987057	0.0
15	0.0	0.0	0.0	3.138480256608782	0.0
16	0.0	0.0	0.0	3.2601482136931756	0.0
17	0.0	0.0	0.0	3.402555027098772	0.0
18	0.0	0.0	0.0	3.6161652472071673	0.0
19	0.0	0.0	0.0	3.71225528149541	0.0
20	0.0	0.0	0.0	3.825627696051322	0.0
21	0.0	0.0	0.0	3.9417652914500607	0.0
22	0.0	0.0	0.0	4.055137706005973	0.0
23	0.0	0.0	0.0	4.212753014047118	0.0
24	0.0	0.0	0.0	4.329581904656565	0.0
25	0.0	0.0	0.0	4.409080853887844	0.0
26	0.0	0.0	0.0	4.507244773808207	0.0
27	0.0	0.0	0.0	4.605408693728569	0.0
28	0.0	0.0	0.0	4.709102975334587	0.0
29	0.0	0.0	0.0	4.805884304833536	0.0
30	0.0	0.0	0.0	4.946217232607013	0.0
31	0.0	0.0	0.0	5.056133171109391	0.0
32	0.0	0.0	0.0	5.175727242561663	0.0
33	0.0	0.0	0.0	5.290482247538989	0.0
34	0.0	0.0	0.0	5.425284813626811	0.0
35	0.0	0.0	0.0	5.5711481030859415	0.0
36	0.0	0.0	0.0	5.68106404158832	0.0
37	0.0	0.0	0.0	5.82070567415109	0.0
38	0.0	0.0	0.0	5.963112487556686	0.0
39	0.0	0.0	0.0	6.138010175865501	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAACGAC	25	3.8738595E-5	45.000004	12
GCGCAAT	25	3.8738595E-5	45.000004	19
TAGGGTA	50	2.1827873E-11	45.000004	5
CGCAATC	25	3.8738595E-5	45.000004	20
AGTAGCG	30	2.1523192E-6	45.000004	1
CAATCGG	25	3.8738595E-5	45.000004	22
ACGACGC	25	3.8738595E-5	45.000004	14
GCAACGA	20	7.012833E-4	45.0	11
TCGTAAG	20	7.012833E-4	45.0	1
GTAACGG	20	7.012833E-4	45.0	2
TGCGTAG	20	7.012833E-4	45.0	1
TAGTAAG	20	7.012833E-4	45.0	1
GTTGATC	115	0.0	43.043476	16
TTGATCC	110	0.0	42.954544	17
GTAGCAT	110	0.0	42.954544	29
ATCCTGC	105	0.0	42.857143	20
AAGGGAC	100	0.0	42.750004	5
CGTTTTT	635	0.0	42.519684	1
TGATCCT	115	0.0	41.086956	18
TAGCGGG	115	0.0	41.086956	3
>>END_MODULE