FastQCFastQC Report
Thu 26 May 2016
SRR1546566_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546566_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2507030
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT375441.49754889251425No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT225000.042.511
TATAGCG1450.040.3448261
CGGTCTA1400.038.5714331
ACGGGTA1650.038.181825
CATATGC19150.037.83289733
TAGCATA19350.037.79069530
TAGGGCG13600.037.720595
ATAGGGC12100.037.5619854
AGGGTAC12300.037.56
GGGCGAT59100.037.461937
GCGATAC1450.037.241389
CGTAAGG3300.036.8181842
GGGTACC21550.036.647337
ATTCGGC3950.036.45569617
CCAGTAG20500.036.43902626
AGGGCGA30750.036.4390226
GTAGCAT20400.036.3970629
GGTACCT20950.036.3007168
TCACGAC1550.036.2903225
GTAGGGC12400.036.290324
Produced by FastQC (version 0.11.5)