##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546566_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2507030
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.413056884042074	31.0	31.0	33.0	30.0	34.0
2	31.7819256251421	31.0	31.0	34.0	30.0	34.0
3	31.78425627136492	31.0	31.0	34.0	30.0	34.0
4	35.61009082460122	37.0	35.0	37.0	33.0	37.0
5	35.56191389811849	37.0	35.0	37.0	33.0	37.0
6	35.60756472798491	37.0	35.0	37.0	33.0	37.0
7	35.9647239961229	37.0	35.0	37.0	35.0	37.0
8	34.62831836874708	37.0	35.0	37.0	33.0	37.0
9	36.907258788287336	39.0	37.0	39.0	33.0	39.0
10	36.871856339972	39.0	37.0	39.0	32.0	39.0
11	36.81341467792567	39.0	37.0	39.0	32.0	39.0
12	36.58658372656091	39.0	35.0	39.0	32.0	39.0
13	36.52598812140261	39.0	35.0	39.0	32.0	39.0
14	37.616771638153516	40.0	36.0	41.0	32.0	41.0
15	37.75922386249865	40.0	36.0	41.0	33.0	41.0
16	37.78752388284145	40.0	36.0	41.0	33.0	41.0
17	37.67952198418048	40.0	36.0	41.0	32.0	41.0
18	37.5325847716222	39.0	36.0	41.0	32.0	41.0
19	37.37619055216731	39.0	36.0	41.0	32.0	41.0
20	37.14644938433126	39.0	35.0	41.0	32.0	41.0
21	37.05190923124175	39.0	35.0	41.0	32.0	41.0
22	36.95905114817134	39.0	35.0	41.0	32.0	41.0
23	36.88663199084175	38.0	35.0	41.0	32.0	41.0
24	36.72838059377032	38.0	35.0	41.0	31.0	41.0
25	36.602602681260294	38.0	35.0	41.0	31.0	41.0
26	36.48656617591333	38.0	35.0	40.0	31.0	41.0
27	36.36503432348237	38.0	35.0	40.0	31.0	41.0
28	36.35857967395683	38.0	35.0	40.0	31.0	41.0
29	36.27218302134398	38.0	35.0	40.0	30.0	41.0
30	36.162009628923464	38.0	35.0	40.0	30.0	41.0
31	35.987667877927265	38.0	35.0	40.0	30.0	41.0
32	35.75331886734502	38.0	35.0	40.0	29.0	41.0
33	35.455139747031346	38.0	35.0	40.0	26.0	41.0
34	35.120380689501125	38.0	34.0	40.0	24.0	41.0
35	34.88927097003227	38.0	34.0	40.0	23.0	41.0
36	34.727081048092764	38.0	34.0	40.0	23.0	41.0
37	34.657953036062594	38.0	34.0	40.0	22.0	41.0
38	34.530995241381234	38.0	34.0	40.0	22.0	41.0
39	34.433865171138756	38.0	34.0	40.0	22.0	41.0
40	34.272130768279595	37.0	33.0	40.0	21.0	41.0
41	34.15389365105324	37.0	33.0	40.0	20.0	41.0
42	34.06092348316534	37.0	33.0	40.0	20.0	41.0
43	33.955821829016806	37.0	33.0	40.0	20.0	41.0
44	33.80694207887421	37.0	33.0	40.0	18.0	41.0
45	33.698221002540855	36.0	33.0	40.0	20.0	41.0
46	33.63896323538211	36.0	33.0	40.0	20.0	41.0
47	33.56934181082795	36.0	33.0	40.0	20.0	41.0
48	33.48723708930488	36.0	33.0	40.0	20.0	41.0
49	33.41759332756289	36.0	33.0	40.0	19.0	41.0
50	33.31512427055121	35.0	33.0	40.0	19.0	41.0
51	32.954132180308974	35.0	32.0	39.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	3.0
11	12.0
12	12.0
13	13.0
14	35.0
15	95.0
16	259.0
17	608.0
18	1386.0
19	2829.0
20	4865.0
21	7738.0
22	11689.0
23	17155.0
24	25361.0
25	38475.0
26	54079.0
27	61777.0
28	61727.0
29	60868.0
30	65029.0
31	74881.0
32	89729.0
33	112718.0
34	177053.0
35	246294.0
36	204749.0
37	257574.0
38	358773.0
39	570787.0
40	455.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.12590196367814	22.75154266203436	25.61668587930739	19.505869494980114
2	38.37520891253794	23.63023178821155	24.954308484541468	13.040250814709037
3	28.428538948476884	24.00944543942434	33.9047797593168	13.657235852781977
4	25.031292006876665	25.89247037331025	33.78934436364941	15.286893256163667
5	22.438423154090696	29.104877085635195	33.00379333314719	15.45290642712692
6	20.663813356840564	37.87788738068551	30.860340721890044	10.597958540583878
7	79.86087123010095	4.1043385998572015	13.587392252984607	2.447397917057235
8	76.96254133376944	6.649381937990372	13.108538788925541	3.2795379393146473
9	73.7641352516723	5.509387602062999	16.090234261257343	4.636242885007359
10	38.64871979992262	23.700913032552464	22.56143723848538	15.08892992903954
11	30.970710362460764	23.641081279442208	28.90332385332445	16.48488450477258
12	26.562865222992944	21.060059113772073	33.67638201377726	18.700693649457726
13	22.771765794585626	23.914911269510135	35.2228732803357	18.09044965556854
14	19.930714829898328	27.03158717685867	35.472052588122196	17.5656454051208
15	18.997858023238653	21.853747262697297	41.01913419464466	18.129260519419393
16	21.753190029636663	20.35400453923567	38.29922258608792	19.593582845039748
17	21.412069261237402	21.226311611747764	33.5108873846743	23.85073174234054
18	23.36306306665656	21.66671320247464	34.84094725631524	20.129276474553556
19	25.817241915732957	23.41439871082516	30.81586578541142	19.95249358803046
20	26.361232215011388	23.449739332995616	29.7788618405045	20.410166611488496
21	24.643622134557624	24.338799296378582	32.17488422555773	18.84269434350606
22	23.398563240168645	21.903527281285026	31.64856423736453	23.049345241181797
23	22.09646474114789	24.538198585577355	30.628951388694993	22.73638528457976
24	21.854864122088685	23.445192119759238	34.495877592210704	20.204066165941374
25	22.677351288177643	23.62428850073593	32.30631464322325	21.392045567863168
26	20.32867576375233	25.247404299110897	31.43177385192838	22.992146085208397
27	20.553882482459322	24.16349225976554	32.50930383760864	22.773321420166493
28	20.79943199722381	24.677127916299366	33.94143668005568	20.582003406421144
29	21.623993330753922	23.630909881413466	33.21428144058907	21.53081534724355
30	22.35078957970188	24.150050059233436	33.29577228832523	20.203388072739457
31	24.58191565318325	23.634459898764675	30.340043796843275	21.4435806512088
32	25.219762029173964	23.98499419631995	30.234899462710857	20.56034431179523
33	24.37425958205526	23.687909598209835	29.727007654475614	22.210823165259292
34	21.533049066026333	25.12462954172866	31.867907444266724	21.474413947978285
35	22.17288983378739	24.8062847273467	31.421083912039343	21.599741526826563
36	25.133364977682753	25.34808119567775	28.708790880045314	20.809762946594176
37	23.19704989569331	26.00168326665417	29.798127665006003	21.003139172646517
38	23.49824294085033	26.54375894983307	28.3703027087829	21.5876954005337
39	23.465375364475097	25.356497528948594	27.739436703988385	23.438690402587923
40	24.013314559458802	23.701631013589786	29.78205286733705	22.503001559614365
41	22.087330426839728	23.828753545031372	29.381578999852415	24.702337028276485
42	23.40378854660694	25.57580084801538	27.93177584632015	23.08863475905753
43	22.995018009357686	24.24514265884333	29.558800652564987	23.201038679233992
44	23.12963945385576	25.15649992221872	29.232518158937072	22.48134246498845
45	21.633845626099408	26.051024519052422	29.219754051606884	23.095375803241286
46	23.0785431366996	25.18501972453461	29.04293127724838	22.693505861517412
47	22.265549275437472	25.84269035472252	29.95185538266395	21.939904987176064
48	22.579386764418455	25.069265226183973	29.775551150165736	22.57579685923184
49	22.74412352464869	23.688787130588786	30.3816069213372	23.185482423425327
50	22.157054363130875	24.99794577647655	30.088152116249105	22.756847744143467
51	21.548007004303898	26.079783648380754	29.31975285497182	23.052456492343527
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2843.0
1	3937.5
2	5032.0
3	33747.5
4	62463.0
5	42743.5
6	23024.0
7	22944.0
8	22864.0
9	23500.5
10	24137.0
11	24154.0
12	24171.0
13	23443.0
14	22715.0
15	21777.0
16	20839.0
17	19915.0
18	18991.0
19	18231.5
20	17472.0
21	17241.5
22	17011.0
23	17156.0
24	17301.0
25	17551.0
26	19105.5
27	20410.0
28	23073.0
29	25736.0
30	28615.5
31	31495.0
32	35506.0
33	39517.0
34	46007.0
35	52497.0
36	57417.0
37	62337.0
38	69001.5
39	75666.0
40	89924.5
41	104183.0
42	113808.0
43	123433.0
44	135680.0
45	147927.0
46	164894.5
47	181862.0
48	186176.5
49	190491.0
50	185166.0
51	179841.0
52	167559.5
53	155278.0
54	144942.5
55	134607.0
56	129907.0
57	125207.0
58	118611.0
59	112015.0
60	107974.0
61	103933.0
62	97106.0
63	90279.0
64	82309.0
65	74339.0
66	64859.5
67	55380.0
68	47830.5
69	40281.0
70	35512.0
71	30743.0
72	26001.0
73	21259.0
74	17810.0
75	11578.5
76	8796.0
77	6516.0
78	4236.0
79	3354.5
80	2473.0
81	1703.5
82	934.0
83	745.5
84	557.0
85	378.5
86	200.0
87	127.5
88	55.0
89	39.5
90	24.0
91	14.0
92	4.0
93	4.5
94	5.0
95	3.5
96	2.0
97	1.5
98	1.0
99	1.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2507030.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.456843616865413
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.87082900444562	21.380871742907306
2	6.2233150934144135	3.4174517859671543
3	2.5376801607111235	2.0903006196680156
4	1.486217994988008	1.6322742027582797
5	0.997714226367683	1.3697041743849665
6	0.7975000824628672	1.3138101029172127
7	0.6922655936918929	1.3305199703134354
8	0.5749241451390149	1.262848187571357
9	0.4841735108545489	1.1964488733865832
>10	7.70645957318136	50.1334701425327
>50	0.5778168743711642	9.75950027950733
>100	0.04511007610107128	2.081309321291635
>500	0.004195564989910811	0.7915677634536166
>1k	0.0016482576746078186	0.6952960084389875
>5k	0.0	0.0
>10k+	1.4984160678252896E-4	1.5446268249013815
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	37544	1.49754889251425	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.03466252896854046	0.0
2	0.0	0.0	0.0	0.15628053912398335	0.0
3	0.0	0.0	0.0	0.33078981902889076	0.0
4	0.0	0.0	0.0	0.5462639058966187	0.0
5	0.0	0.0	0.0	0.97613510807609	0.0
6	0.0	0.0	0.0	1.7750884512750147	0.0
7	0.0	0.0	0.0	2.2442491713302193	0.0
8	0.0	0.0	0.0	3.183926797844461	0.0
9	0.0	0.0	0.0	3.824126556124179	0.0
10	0.0	0.0	0.0	4.69372125582861	0.0
11	0.0	0.0	0.0	5.189247835087733	0.0
12	0.0	0.0	0.0	5.621911185745683	0.0
13	0.0	0.0	0.0	5.859164030745544	0.0
14	0.0	0.0	0.0	5.989876467373745	0.0
15	0.0	0.0	0.0	6.092787082723381	0.0
16	0.0	0.0	0.0	6.256646310574664	0.0
17	0.0	0.0	0.0	6.422420154525474	0.0
18	0.0	0.0	0.0	6.685839419552219	0.0
19	3.988783540683598E-5	0.0	0.0	6.805981579797609	0.0
20	3.988783540683598E-5	0.0	0.0	6.951651954703374	0.0
21	3.988783540683598E-5	0.0	0.0	7.095407713509611	0.0
22	3.988783540683598E-5	0.0	0.0	7.242234835642174	0.0
23	3.988783540683598E-5	0.0	0.0	7.404338998735556	0.0
24	3.988783540683598E-5	0.0	0.0	7.542550348420242	0.0
25	3.988783540683598E-5	0.0	0.0	7.658464398112508	0.0
26	3.988783540683598E-5	0.0	0.0	7.775614970702385	0.0
27	3.988783540683598E-5	0.0	0.0	7.9232398495430845	0.0
28	3.988783540683598E-5	0.0	0.0	8.04912585808706	0.0
29	3.988783540683598E-5	0.0	0.0	8.18426584444542	0.0
30	3.988783540683598E-5	0.0	0.0	8.369465064239359	0.0
31	7.977567081367196E-5	0.0	0.0	8.512861832526934	0.0
32	7.977567081367196E-5	0.0	0.0	8.661124916734144	0.0
33	7.977567081367196E-5	0.0	0.0	8.812938018292561	0.0
34	7.977567081367196E-5	0.0	0.0	8.96247751323279	0.0
35	7.977567081367196E-5	0.0	0.0	9.132359804230504	0.0
36	7.977567081367196E-5	0.0	0.0	9.285090326003278	0.0
37	7.977567081367196E-5	0.0	0.0	9.446675947236372	0.0
38	7.977567081367196E-5	0.0	0.0	9.622102647355636	0.0
39	7.977567081367196E-5	0.0	0.0	9.888314060860859	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	22500	0.0	42.51	1
TATAGCG	145	0.0	40.344826	1
CGGTCTA	140	0.0	38.57143	31
ACGGGTA	165	0.0	38.18182	5
CATATGC	1915	0.0	37.832897	33
TAGCATA	1935	0.0	37.790695	30
TAGGGCG	1360	0.0	37.72059	5
ATAGGGC	1210	0.0	37.561985	4
AGGGTAC	1230	0.0	37.5	6
GGGCGAT	5910	0.0	37.46193	7
GCGATAC	145	0.0	37.24138	9
CGTAAGG	330	0.0	36.818184	2
GGGTACC	2155	0.0	36.64733	7
ATTCGGC	395	0.0	36.455696	17
CCAGTAG	2050	0.0	36.439026	26
AGGGCGA	3075	0.0	36.439022	6
GTAGCAT	2040	0.0	36.39706	29
GGTACCT	2095	0.0	36.300716	8
TCACGAC	155	0.0	36.29032	25
GTAGGGC	1240	0.0	36.29032	4
>>END_MODULE