FastQCFastQC Report
Thu 26 May 2016
SRR1546565_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546565_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3090186
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT210960.6826773534020283No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC41920.13565526476399803No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACGT207.0351386E-445.030
CGTTTTT146250.040.9230771
TACGCAA403.4607547E-739.3751
AGGGCGA39500.039.3037956
AGGGTAC20350.039.2506146
TAACGCC4550.039.06593312
CAGTAGC32150.038.9813427
GTTGATC32650.038.86676816
CATATGC30700.038.84364733
AGCATAT30750.038.78048731
TAGCATA30700.038.7703630
TCACGAC2850.038.68420825
GGGTACC35200.038.6718757
GGGCGAT68750.038.5527277
AGTAGCA32300.038.38235528
TTGATCC33400.038.1961117
CGTAAGG4600.038.1521722
GACACGA17500.038.0571425
GTAGCAT32600.038.0291429
ATATGCT31150.037.9935834
Produced by FastQC (version 0.11.5)