##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546565_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3090186
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.386551489133662	31.0	31.0	33.0	30.0	34.0
2	31.745568713339587	31.0	31.0	34.0	30.0	34.0
3	31.83349222344545	31.0	31.0	34.0	30.0	34.0
4	35.61112437892088	37.0	35.0	37.0	33.0	37.0
5	35.54111823689577	37.0	35.0	37.0	33.0	37.0
6	35.59045895619228	37.0	35.0	37.0	33.0	37.0
7	35.969174023828984	37.0	35.0	37.0	35.0	37.0
8	34.648128623972795	37.0	35.0	37.0	33.0	37.0
9	36.9278454436076	39.0	37.0	39.0	33.0	39.0
10	36.819302786304775	39.0	37.0	39.0	32.0	39.0
11	36.760409891184544	39.0	35.0	39.0	32.0	39.0
12	36.413660213333436	39.0	35.0	39.0	32.0	39.0
13	36.276350355609665	39.0	35.0	39.0	32.0	39.0
14	37.340767190065584	39.0	35.0	41.0	32.0	41.0
15	37.51755266511466	40.0	35.0	41.0	32.0	41.0
16	37.57116691357737	40.0	35.0	41.0	33.0	41.0
17	37.44926163020608	39.0	35.0	41.0	32.0	41.0
18	37.38489139488691	39.0	36.0	41.0	32.0	41.0
19	37.29649509770609	39.0	35.0	41.0	32.0	41.0
20	37.160014639895465	39.0	35.0	41.0	32.0	41.0
21	37.04634834278584	39.0	35.0	41.0	32.0	41.0
22	36.95637641229363	39.0	35.0	41.0	31.0	41.0
23	36.872763969547464	38.0	35.0	41.0	31.0	41.0
24	36.72555341328969	38.0	35.0	41.0	31.0	41.0
25	36.613692832729164	38.0	35.0	41.0	31.0	41.0
26	36.48356215451109	38.0	35.0	40.0	31.0	41.0
27	36.3627470967767	38.0	35.0	40.0	30.0	41.0
28	36.3665031813619	38.0	35.0	40.0	30.0	41.0
29	36.317951411338996	38.0	35.0	40.0	30.0	41.0
30	36.20832144084531	38.0	35.0	40.0	30.0	41.0
31	36.1013608889562	38.0	35.0	40.0	30.0	41.0
32	35.97222303123501	38.0	35.0	40.0	30.0	41.0
33	35.806552744721515	38.0	35.0	41.0	29.0	41.0
34	35.621687173522886	38.0	35.0	40.0	28.0	41.0
35	35.50750310822714	38.0	35.0	41.0	27.0	41.0
36	35.35231180259052	38.0	35.0	40.0	26.0	41.0
37	35.30580262806187	38.0	34.0	40.0	26.0	41.0
38	35.18148486854837	38.0	34.0	40.0	26.0	41.0
39	35.117902611687455	38.0	34.0	40.0	26.0	41.0
40	34.969032284788035	37.0	34.0	40.0	25.0	41.0
41	34.88629163422525	37.0	34.0	40.0	25.0	41.0
42	34.80233908250183	37.0	34.0	40.0	24.0	41.0
43	34.70106459611169	37.0	34.0	40.0	24.0	41.0
44	34.5613442038764	37.0	34.0	40.0	24.0	41.0
45	34.42605493649897	36.0	34.0	40.0	23.0	41.0
46	34.35302276303109	36.0	34.0	40.0	23.0	41.0
47	34.27327643060968	36.0	33.0	40.0	23.0	41.0
48	34.20210045608905	36.0	33.0	40.0	23.0	41.0
49	34.132383293432824	36.0	33.0	40.0	24.0	41.0
50	34.0180193036924	35.0	33.0	40.0	23.0	41.0
51	33.64023039389862	35.0	33.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	3.0
10	6.0
11	12.0
12	8.0
13	15.0
14	34.0
15	135.0
16	306.0
17	738.0
18	1634.0
19	3092.0
20	5279.0
21	8257.0
22	12501.0
23	18180.0
24	26295.0
25	37805.0
26	50053.0
27	58117.0
28	61722.0
29	67732.0
30	77511.0
31	92583.0
32	113932.0
33	144925.0
34	234529.0
35	350258.0
36	247419.0
37	318304.0
38	449606.0
39	708627.0
40	567.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.7844757564755	23.4265510231423	26.797092472750833	14.991880747631372
2	33.83304435396445	24.432251003661268	28.093163324149423	13.64154131822486
3	29.83535618891549	25.06457540096292	30.872251702648317	14.227816707473274
4	25.68389087258825	27.514233771041614	30.67488494220089	16.126990414169246
5	22.875160265433863	31.379502722489843	30.147052636961014	15.598284375115284
6	20.928222443568124	40.556555495365004	27.58850114523851	10.926720915828367
7	84.96656835543233	4.303915686628572	8.571134553065738	2.1583814048733636
8	82.07593329333575	6.476632798155192	8.304678100282636	3.14275580822643
9	79.14397385788429	5.468991186938262	11.43222446804173	3.9548104871357257
10	41.620957443985574	25.265242933596877	18.265696627969966	14.848102994447585
11	35.20794541170014	24.75475586259209	23.976647360385428	16.060651365322347
12	30.911149037630743	21.63203121106626	28.15817559201938	19.298644159283615
13	23.823841024456133	28.182154731139157	29.904478241762796	18.08952600264191
14	20.46585545336106	30.70371815806557	30.852188185436084	17.978238203137288
15	19.43669410190843	23.018517331966425	39.21103778219175	18.333750783933393
16	22.055371424244367	20.571383081795076	37.322251799729855	20.050993694230705
17	21.258073138639553	21.19399932560694	30.55434203636933	26.99358549938418
18	24.009687442762345	22.169248064679604	32.836437677214256	20.984626815343802
19	27.6163635457542	24.70449998802661	26.85498542806161	20.824151038157574
20	29.06407575466331	24.259640034612804	25.45002145501921	21.226262755704674
21	25.16453702139612	26.19884369419834	28.734678106754743	19.901941177650794
22	24.80750349655328	22.108313221275353	27.81706343889979	25.26711984327157
23	22.533595065151417	26.63360716798277	26.943847393004823	23.88895037386099
24	22.6653670685195	24.45723331864166	32.86776265247464	20.0096369603642
25	23.141616718216962	24.165406224738575	30.133687745656733	22.55928931138773
26	20.635651057897487	27.65432242589928	28.015692259300895	23.694334256902337
27	21.790241752438202	25.580919724573214	28.813443592068566	23.815394930920018
28	20.02047773176113	26.459378173352672	32.46519788776469	21.054946207121514
29	21.68756184902786	24.850769500606113	30.62559988298439	22.83606876738164
30	23.010135959453574	25.070950421754546	30.578709501628705	21.340204117163175
31	27.23522143974505	25.199195129354674	25.771037730414932	21.794545700485344
32	27.461809742196746	24.70045492407253	26.214473821316904	21.623261512413816
33	25.777833437857783	25.26899675294626	25.204243369169365	23.748926440026587
34	22.173066605052252	27.211792429323022	28.021743674976197	22.59339729064852
35	22.78775452351412	25.7819108623235	28.443724746665733	22.98660986749665
36	26.73153007618312	26.73822870209107	25.599721181831775	20.930520039894038
37	23.187115597572443	27.333759197666417	26.91614679504729	22.56297840971385
38	24.11508562914983	28.68629914186395	24.90215799307873	22.296457235907482
39	22.795197441189625	26.456562808840633	25.527880846007328	25.220358903962413
40	24.00541585522684	24.33811427532194	27.329843575758872	24.326626293692357
41	20.7259045248409	23.749508929235972	28.03070753669844	27.493879009224685
42	23.638124048196453	25.41727261724699	25.813235837583886	25.131367496972672
43	22.879140608364672	24.76718229905902	27.704966626604353	24.648710465971952
44	22.781508944769023	26.598657815419525	27.46074184531287	23.159091394498585
45	21.64283962195156	27.58374415002851	26.864240534388546	23.909175693631386
46	23.677344988295204	26.420092512230653	27.150825225407143	22.751737274066997
47	22.808950658633492	26.08341374920474	28.00711672371825	23.10051886844352
48	23.183555941292855	25.408082231943318	28.785807715134297	22.622554111629526
49	23.185303408921015	23.93396384554198	28.60578618892196	24.27494655661504
50	22.501331635053685	26.327897414589284	28.047081955584552	23.123688994772483
51	21.646075673114822	27.481776177874085	26.681791969803758	24.19035617920734
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3276.0
1	4461.5
2	5647.0
3	21314.5
4	36982.0
5	25991.0
6	15000.0
7	15422.0
8	15844.0
9	16808.0
10	17772.0
11	18055.5
12	18339.0
13	18280.0
14	18221.0
15	17474.0
16	16727.0
17	16592.0
18	16457.0
19	15893.5
20	15330.0
21	15019.0
22	14708.0
23	15446.5
24	16185.0
25	16630.0
26	20221.5
27	23368.0
28	26611.0
29	29854.0
30	33975.5
31	38097.0
32	40695.5
33	43294.0
34	50469.0
35	57644.0
36	67828.5
37	78013.0
38	85211.5
39	92410.0
40	112402.5
41	132395.0
42	156145.0
43	179895.0
44	190913.0
45	201931.0
46	210323.0
47	218715.0
48	233912.5
49	249110.0
50	253417.0
51	257724.0
52	249291.5
53	240859.0
54	215906.0
55	190953.0
56	176526.5
57	162100.0
58	151330.5
59	140561.0
60	135038.0
61	129515.0
62	116486.5
63	103458.0
64	95720.0
65	87982.0
66	76080.5
67	64179.0
68	55580.5
69	46982.0
70	40217.0
71	33452.0
72	28977.0
73	24502.0
74	20029.0
75	12598.0
76	9640.0
77	7381.5
78	5123.0
79	3908.5
80	2694.0
81	2131.5
82	1569.0
83	1081.5
84	594.0
85	423.0
86	252.0
87	182.5
88	113.0
89	81.0
90	49.0
91	33.5
92	18.0
93	13.5
94	9.0
95	8.0
96	7.0
97	6.5
98	6.0
99	3.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3090186.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.94457770493811
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.57945071126714	18.48861468265899
2	6.89855329449397	3.1656878423434733
3	2.3381599669675497	1.609442791459874
4	1.208281984361081	1.1089407951859853
5	0.8086319842477258	0.9276859698635115
6	0.5696414106868043	0.7842108966872364
7	0.4614675455537575	0.7411724570085678
8	0.37159295154945793	0.6820834681147071
9	0.31528579280302127	0.6510689435008743
>10	4.0694816012028765	23.0706226082603
>50	1.8206345451448152	29.689498328537038
>100	0.5522203840904542	16.913449824326843
>500	0.004251933362396467	0.686309446062693
>1k	0.002199275877101621	0.7715220251949316
>5k	0.0	0.0
>10k+	1.4661839180677477E-4	0.709689920794989
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	21096	0.6826773534020283	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	4192	0.13565526476399803	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.05679269791527112	0.0
2	0.0	0.0	0.0	0.18002152621233802	0.0
3	0.0	0.0	0.0	0.28946477655390324	0.0
4	0.0	0.0	0.0	0.48065067927949967	0.0
5	0.0	0.0	0.0	0.8135756229560291	0.0
6	0.0	0.0	0.0	1.4527280882121658	0.0
7	0.0	0.0	0.0	1.829080838499689	0.0
8	0.0	0.0	0.0	2.836398844600293	0.0
9	0.0	0.0	0.0	3.3603478884442555	0.0
10	0.0	0.0	0.0	4.12162892460195	0.0
11	0.0	0.0	0.0	4.615515053139196	0.0
12	0.0	0.0	0.0	5.049113548504847	0.0
13	0.0	0.0	0.0	5.25279060872064	0.0
14	0.0	0.0	0.0	5.344144333059563	0.0
15	0.0	0.0	0.0	5.440384494655015	0.0
16	0.0	0.0	0.0	5.630114174357142	0.0
17	0.0	0.0	0.0	5.831623080293548	0.0
18	0.0	0.0	0.0	6.139856953594379	0.0
19	0.0	0.0	0.0	6.2713700728694	0.0
20	0.0	0.0	0.0	6.4123648220527825	0.0
21	0.0	0.0	0.0	6.595784201986547	0.0
22	0.0	0.0	0.0	6.776582380478068	0.0
23	0.0	0.0	0.0	6.985696006648143	0.0
24	0.0	0.0	0.0	7.1520613969515106	0.0
25	0.0	0.0	0.0	7.2925707384604035	0.0
26	0.0	0.0	0.0	7.429358621131543	0.0
27	0.0	0.0	0.0	7.569932683663702	0.0
28	0.0	0.0	0.0	7.71885575819708	0.0
29	0.0	0.0	0.0	7.87949333794147	0.0
30	0.0	0.0	0.0	8.092652028065624	0.0
31	0.0	0.0	0.0	8.262771237718377	0.0
32	0.0	0.0	0.0	8.433861262720107	0.0
33	0.0	0.0	0.0	8.61122922697857	0.0
34	3.236051163263312E-5	0.0	0.0	8.7839049170503	0.0
35	3.236051163263312E-5	0.0	0.0	8.98188652721875	0.0
36	3.236051163263312E-5	0.0	0.0	9.158024792035173	0.0
37	3.236051163263312E-5	0.0	0.0	9.341444171968936	0.0
38	3.236051163263312E-5	0.0	0.0	9.554020372883704	0.0
39	3.236051163263312E-5	0.0	0.0	9.82436008706272	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTACGT	20	7.0351386E-4	45.0	30
CGTTTTT	14625	0.0	40.923077	1
TACGCAA	40	3.4607547E-7	39.375	1
AGGGCGA	3950	0.0	39.303795	6
AGGGTAC	2035	0.0	39.250614	6
TAACGCC	455	0.0	39.065933	12
CAGTAGC	3215	0.0	38.98134	27
GTTGATC	3265	0.0	38.866768	16
CATATGC	3070	0.0	38.843647	33
AGCATAT	3075	0.0	38.780487	31
TAGCATA	3070	0.0	38.77036	30
TCACGAC	285	0.0	38.684208	25
GGGTACC	3520	0.0	38.671875	7
GGGCGAT	6875	0.0	38.552727	7
AGTAGCA	3230	0.0	38.382355	28
TTGATCC	3340	0.0	38.19611	17
CGTAAGG	460	0.0	38.152172	2
GACACGA	1750	0.0	38.05714	25
GTAGCAT	3260	0.0	38.02914	29
ATATGCT	3115	0.0	37.99358	34
>>END_MODULE