FastQCFastQC Report
Thu 26 May 2016
SRR1546564_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546564_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2121291
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT288181.3585123398911323No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT185600.042.9876061
TGGGCGA13300.039.0789456
GGCGATA9050.039.033158
GCGTAAG1100.038.8636361
TCTAGCG1050.038.571431
TATCGCG356.2507897E-638.5714261
TACGGGA6700.038.2835854
GGGCGAT43950.037.679187
TCGATAG1150.037.1739121
CGGTCTA1400.036.96428331
GTTTTTT218050.036.775972
CATATGC17750.036.50704233
AAGGGAT25800.036.4534875
TATGGGA17050.036.422294
CGTTCGG5500.036.40909245
TAGGGAC18600.036.2903255
AACACGT6200.036.2903241
AGGGCGA22650.036.2582786
GACCGAT12600.036.071439
TAGGGAT21850.036.041195
Produced by FastQC (version 0.11.5)