FastQCFastQC Report
Thu 26 May 2016
SRR1546563_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546563_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2895105
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT449141.5513772384766702No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTT44040.15211883506815815No Hit
CGTTCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTC30070.10386497208218701No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCT29860.10313960978962766No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT366050.043.3526841
TAATGCG1050.040.7142871
CGTAAGG3450.039.7826082
CGACGGT1650.039.54545628
TACGGGA7150.039.3356634
GACCGAT17400.039.1810349
AGTACGG3100.038.467742
TAGCATA18200.038.32417330
GTTTTTT410350.038.0802962
GCGATAT3850.037.9870159
TGATTCG3550.037.39436715
TAACGCC2650.037.3584912
AGGGCGA33950.037.245956
GGACCGA18500.037.0945938
GTTGATC18950.037.0448516
GGGTACC21300.036.9718327
CCAGTAG19050.036.96850626
GTAGCAT18950.036.92611729
GGGCGAT68750.036.7854547
AGCATAT19100.036.63612731