##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546563_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2895105
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.431524936055858	31.0	31.0	33.0	30.0	34.0
2	31.791931898843046	31.0	31.0	34.0	30.0	34.0
3	31.716954998177957	31.0	31.0	34.0	30.0	34.0
4	35.5883106139501	37.0	35.0	37.0	33.0	37.0
5	35.558290286535374	37.0	35.0	37.0	33.0	37.0
6	35.60923317116305	37.0	35.0	37.0	33.0	37.0
7	36.0113329222947	37.0	35.0	37.0	35.0	37.0
8	34.692553465245645	37.0	35.0	37.0	33.0	37.0
9	36.979991399275676	39.0	37.0	39.0	33.0	39.0
10	36.8735469007169	39.0	37.0	39.0	32.0	39.0
11	36.86037190360971	39.0	37.0	39.0	32.0	39.0
12	36.68978672621546	39.0	35.0	39.0	32.0	39.0
13	36.6699801907012	39.0	35.0	39.0	32.0	39.0
14	37.817507137046846	40.0	36.0	41.0	33.0	41.0
15	37.92230299073781	40.0	36.0	41.0	33.0	41.0
16	37.9217969641861	40.0	36.0	41.0	33.0	41.0
17	37.79795551456683	40.0	36.0	41.0	33.0	41.0
18	37.58901974194373	39.0	36.0	41.0	33.0	41.0
19	37.39196609449398	39.0	36.0	41.0	32.0	41.0
20	37.124037297438264	39.0	35.0	41.0	32.0	41.0
21	37.03222024762487	39.0	35.0	41.0	32.0	41.0
22	36.93994345628224	38.0	35.0	41.0	32.0	41.0
23	36.851796394258585	38.0	35.0	41.0	32.0	41.0
24	36.68594506934981	38.0	35.0	41.0	31.0	41.0
25	36.591564036537534	38.0	35.0	40.0	31.0	41.0
26	36.49955701088562	38.0	35.0	40.0	31.0	41.0
27	36.38091744513584	38.0	35.0	40.0	31.0	41.0
28	36.34964569506115	38.0	35.0	40.0	31.0	41.0
29	36.25182713580337	38.0	35.0	40.0	31.0	41.0
30	36.110396686821375	38.0	35.0	40.0	30.0	41.0
31	35.86544909424701	38.0	35.0	40.0	30.0	41.0
32	35.50598510244016	38.0	35.0	40.0	27.0	41.0
33	35.024008110241255	38.0	34.0	40.0	23.0	41.0
34	34.56587584906247	38.0	34.0	40.0	21.0	41.0
35	34.211636883636345	38.0	33.0	40.0	18.0	41.0
36	34.00287416173161	38.0	33.0	40.0	18.0	41.0
37	33.930903024242646	38.0	33.0	40.0	17.0	41.0
38	33.78823324197223	38.0	33.0	40.0	15.0	41.0
39	33.699293807996604	38.0	33.0	40.0	15.0	41.0
40	33.56176200863181	37.0	33.0	40.0	15.0	41.0
41	33.44565257564061	37.0	33.0	40.0	15.0	41.0
42	33.35413706929455	37.0	33.0	40.0	15.0	41.0
43	33.25223161163412	37.0	33.0	40.0	13.0	41.0
44	33.10694568936187	37.0	32.0	40.0	12.0	41.0
45	32.971382730505454	36.0	32.0	40.0	12.0	41.0
46	32.87571435232919	36.0	32.0	40.0	12.0	41.0
47	32.78213536296611	36.0	32.0	40.0	12.0	41.0
48	32.66456553389256	36.0	32.0	40.0	11.0	41.0
49	32.56485308822996	35.0	31.0	40.0	11.0	41.0
50	32.44079955649277	35.0	31.0	40.0	10.0	41.0
51	32.077302204928664	35.0	31.0	39.0	10.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	3.0
11	6.0
12	9.0
13	13.0
14	30.0
15	115.0
16	319.0
17	800.0
18	1788.0
19	3652.0
20	6342.0
21	10090.0
22	15220.0
23	23191.0
24	36402.0
25	58663.0
26	81919.0
27	88813.0
28	81308.0
29	75524.0
30	76804.0
31	85493.0
32	100235.0
33	123486.0
34	186772.0
35	262779.0
36	222669.0
37	281146.0
38	408885.0
39	662134.0
40	492.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.819607578999726	21.52719849539136	25.036535807854982	22.616658117753932
2	40.31200940898517	22.762075986881307	24.331207330994904	12.594707273138624
3	26.868248301875063	23.187414618813477	37.140967253346595	12.803369825964861
4	23.424020890434026	25.575169121672616	37.079898656525415	13.920911331367947
5	21.44354004431618	28.46052215722746	36.079520431901436	14.016417366554926
6	18.857139896480437	36.745023064793855	34.0645330652947	10.333303973431015
7	78.25481286516379	3.5012201629992696	16.299823322470168	1.9441436493667759
8	75.78264000787536	5.943238673554154	15.583372623790847	2.6907486947796366
9	72.86233141803147	4.972323974432706	18.505166479281407	3.6601781282544152
10	33.32279830956045	25.60732684997608	26.438937447864586	14.630937392598886
11	28.30601998891232	24.59285587223952	31.991723961652514	15.109400177195646
12	24.700658525338458	22.019270458238992	35.614459579186246	17.6656114372363
13	21.799071190854907	24.595446451855803	36.854276442477904	16.75120591481138
14	19.321475386903067	27.119465442531443	36.80025422221301	16.758804948352477
15	18.94642854058834	23.188036357921387	41.66667530193205	16.19885979955822
16	20.881487890767346	21.575037865638723	39.89734396507208	17.64613027852185
17	20.23553549871248	22.394351845615272	36.19039723947836	21.179715416193886
18	22.081824320706847	22.43151802784355	37.075511941708506	18.4111457097411
19	23.946005412584345	25.13231817153437	33.26342222475523	17.658254191126055
20	24.998782427580345	23.721315807198703	32.80554591284254	18.47435585237841
21	23.68857088084888	24.939682671267533	34.2627642175327	17.108982230350886
22	22.229763687327402	22.518354256581368	33.91918427829042	21.332697777800806
23	20.557423651301075	25.84427853221213	33.32279830956045	20.275499506926348
24	20.305584771536783	24.400462159403542	36.84778272290642	18.44617034615325
25	20.870607456378956	24.624806354173685	35.07938399470831	19.42520219473905
26	19.355809202084206	25.8767816711311	34.29595817768267	20.471450949102017
27	19.639874892275063	24.82182856925742	34.78771927097635	20.750577267491163
28	19.201859690753874	25.52598265002478	36.40413732835251	18.86802033086883
29	20.6212555330463	23.950288504216598	35.10048858331563	20.327967379421473
30	22.04524533652493	24.195219171670804	35.09938326934602	18.660152222458255
31	23.39027427329924	24.872051272751765	32.28729182533967	19.450382628609326
32	24.724180988254314	24.346129069584695	31.821851020947427	19.107838921213567
33	23.442638522609716	25.19169425633958	30.521794546311792	20.84387267473891
34	21.8178960693999	26.49230338796002	31.675535084219742	20.014265458420333
35	22.45390063572824	26.417936482441917	31.070341144794405	20.057821737035443
36	24.349237765124236	27.803758412907303	28.36062940722357	19.48637441474489
37	23.201196502372106	28.10568183191974	29.19648855568278	19.49663311002537
38	22.469236867056637	28.209201393386422	29.228439037616944	20.09312270194
39	22.568369713706414	27.550606972804093	28.20519463024657	21.675828683242923
40	23.015296509107614	25.984618865291587	30.09065301603914	20.909431609561658
41	20.597594905884243	26.417176579087805	30.244775232677224	22.740453282350725
42	22.55510594607104	27.294623165653746	28.479865151695705	21.6704057365795
43	21.863490270646487	26.226889871006403	29.986442633341447	21.923177225005656
44	22.22413349429468	27.194661333526764	29.149961745774334	21.431243426404222
45	21.16317024771122	27.345433067194453	29.220114641783283	22.271282043311036
46	22.620526716647582	26.9023057885638	28.83062963174047	21.646537863048145
47	22.258467309475822	26.719307244469544	30.20370591049375	20.81851953556089
48	22.32502793508353	26.016154854487144	30.020189250476236	21.638627959953094
49	21.997060555662056	25.650986751775847	30.05400494973412	22.29794774282798
50	21.32544415487521	26.157807747905515	30.610323287065583	21.90642481015369
51	21.341851159111673	26.59147077567135	29.557891682685085	22.508786382531895
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3160.0
1	3909.0
2	4658.0
3	41354.5
4	78051.0
5	55847.5
6	33644.0
7	33825.5
8	34007.0
9	35371.0
10	36735.0
11	37064.0
12	37393.0
13	36952.5
14	36512.0
15	35119.0
16	33726.0
17	32362.5
18	30999.0
19	29583.5
20	28168.0
21	27085.5
22	26003.0
23	25544.0
24	25085.0
25	25311.5
26	26826.5
27	28115.0
28	30628.5
29	33142.0
30	36646.0
31	40150.0
32	46455.5
33	52761.0
34	59978.0
35	67195.0
36	74547.5
37	81900.0
38	91537.5
39	101175.0
40	112906.5
41	124638.0
42	138497.0
43	152356.0
44	160016.5
45	167677.0
46	175897.5
47	184118.0
48	200564.5
49	217011.0
50	225344.0
51	233677.0
52	224205.5
53	214734.0
54	189750.0
55	164766.0
56	148982.5
57	133199.0
58	122720.0
59	112241.0
60	103329.5
61	94418.0
62	84577.0
63	74736.0
64	66630.0
65	58524.0
66	48925.5
67	39327.0
68	33335.0
69	27343.0
70	23503.0
71	19663.0
72	17648.5
73	15634.0
74	13029.5
75	8198.5
76	5972.0
77	4723.5
78	3475.0
79	2607.0
80	1739.0
81	1217.0
82	695.0
83	493.5
84	292.0
85	232.0
86	172.0
87	119.0
88	66.0
89	60.5
90	55.0
91	39.5
92	24.0
93	17.5
94	11.0
95	5.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2895105.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.819511533302034
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.6301816437014	24.23343790033689
2	6.4814032228987735	3.9950736276021948
3	2.689000331641921	2.4862103020227355
4	1.638648617304627	2.0200939984019755
5	1.0952564170599892	1.6877633888751704
6	0.8617363780344534	1.593497654489925
7	0.6900971510145827	1.4887919973354966
8	0.5850104615501115	1.4423789333476802
9	0.4954447507355917	1.3742428688468569
>10	6.258491449596824	43.902849762173304
>50	0.5182836258377427	9.98284177875967
>100	0.05184283731847686	2.636128550411611
>500	0.0029920236485655653	0.6211589832454577
>1k	0.0014960118242827048	0.9425912011941224
>5k	0.0	0.0
>10k+	1.1507783263713115E-4	1.5929390529567977
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	44914	1.5513772384766702	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTT	4404	0.15211883506815815	No Hit
CGTTCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTC	3007	0.10386497208218701	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCT	2986	0.10313960978962766	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.908212310088926E-5	0.0	0.0	0.048012075555118036	0.0
2	6.908212310088926E-5	0.0	0.0	0.18216955861704498	0.0
3	6.908212310088926E-5	0.0	0.0	0.38299129047133007	0.0
4	6.908212310088926E-5	0.0	0.0	0.5791845200778556	0.0
5	6.908212310088926E-5	0.0	0.0	0.9636610761958547	0.0
6	6.908212310088926E-5	0.0	0.0	1.7810407567255764	0.0
7	6.908212310088926E-5	0.0	0.0	2.286031076593077	0.0
8	6.908212310088926E-5	0.0	0.0	3.2223356320409797	0.0
9	6.908212310088926E-5	0.0	0.0	3.8845223230245534	0.0
10	6.908212310088926E-5	0.0	0.0	4.7954046571713285	0.0
11	6.908212310088926E-5	0.0	0.0	5.346541835270223	0.0
12	6.908212310088926E-5	0.0	0.0	5.824348339697524	0.0
13	6.908212310088926E-5	0.0	0.0	6.099606059193017	0.0
14	6.908212310088926E-5	0.0	0.0	6.2358705470095215	0.0
15	6.908212310088926E-5	0.0	0.0	6.349579721633585	0.0
16	1.0362318465133389E-4	0.0	0.0	6.5357560433904816	0.0
17	1.3816424620177853E-4	0.0	0.0	6.728495166841962	0.0
18	1.3816424620177853E-4	0.0	0.0	7.036981387548984	0.0
19	1.3816424620177853E-4	0.0	0.0	7.174282107212001	0.0
20	1.3816424620177853E-4	0.0	0.0	7.334345386436762	0.0
21	1.3816424620177853E-4	0.0	0.0	7.493890549738265	0.0
22	1.3816424620177853E-4	0.0	0.0	7.670222668953285	0.0
23	1.3816424620177853E-4	0.0	0.0	7.876812758086494	0.0
24	1.3816424620177853E-4	0.0	0.0	8.047169273653287	0.0
25	1.3816424620177853E-4	0.0	0.0	8.193830620996476	0.0
26	1.3816424620177853E-4	0.0	0.0	8.335518055476399	0.0
27	1.3816424620177853E-4	0.0	0.0	8.50338761461156	0.0
28	1.3816424620177853E-4	0.0	0.0	8.657371667003442	0.0
29	1.3816424620177853E-4	0.0	0.0	8.825310308261704	0.0
30	1.3816424620177853E-4	0.0	0.0	9.05497382651061	0.0
31	1.7270530775222314E-4	0.0	0.0	9.226159327554614	0.0
32	1.7270530775222314E-4	0.0	0.0	9.40055714732281	0.0
33	1.7270530775222314E-4	0.0	0.0	9.578409073246048	0.0
34	1.7270530775222314E-4	0.0	0.0	9.75097621675207	0.0
35	1.7270530775222314E-4	0.0	0.0	9.940433939356259	0.0
36	1.7270530775222314E-4	0.0	0.0	10.115626203540113	0.0
37	1.7270530775222314E-4	0.0	0.0	10.302355182281817	0.0
38	1.7270530775222314E-4	0.0	0.0	10.508116285937815	0.0
39	1.7270530775222314E-4	0.0	0.0	10.751181736068295	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	36605	0.0	43.352684	1
TAATGCG	105	0.0	40.714287	1
CGTAAGG	345	0.0	39.782608	2
CGACGGT	165	0.0	39.545456	28
TACGGGA	715	0.0	39.335663	4
GACCGAT	1740	0.0	39.181034	9
AGTACGG	310	0.0	38.46774	2
TAGCATA	1820	0.0	38.324173	30
GTTTTTT	41035	0.0	38.080296	2
GCGATAT	385	0.0	37.987015	9
TGATTCG	355	0.0	37.394367	15
TAACGCC	265	0.0	37.35849	12
AGGGCGA	3395	0.0	37.24595	6
GGACCGA	1850	0.0	37.094593	8
GTTGATC	1895	0.0	37.04485	16
GGGTACC	2130	0.0	36.971832	7
CCAGTAG	1905	0.0	36.968506	26
GTAGCAT	1895	0.0	36.926117	29
GGGCGAT	6875	0.0	36.785454	7
AGCATAT	1910	0.0	36.636127	31
>>END_MODULE