FastQCFastQC Report
Thu 26 May 2016
SRR1546562_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546562_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences552414
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39400.7132331910487425No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCACGT351.2103919E-745.00000445
TCCGCAA207.0301007E-445.00000432
ACGTTAG207.0301007E-445.0000041
CACGACG351.2103919E-745.00000426
GTTACAC502.1827873E-1145.00000433
AATGCGT207.0301007E-445.00000429
TACACGG207.0301007E-445.0000042
CCGCAAA302.163446E-645.00000441
TGATTCG650.041.5384615
TAGGGCG2900.041.120695
CGTTTTT24800.041.0080641
CGGGTAT501.0786607E-940.5000046
TTTACGG451.9252184E-840.02
TAACGCC451.9252184E-840.012
ACGGGAT900.040.05
ATAACGC451.9252184E-840.011
CGCATCG403.453879E-739.37500421
CGGTCTA403.453879E-739.37500431
CGTTTAT403.453879E-739.37500439
ATAGGGC2750.039.2727284