##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546562_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	552414
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.377501294319117	31.0	31.0	33.0	30.0	34.0
2	31.745833740636552	31.0	31.0	34.0	30.0	34.0
3	31.850988570166578	31.0	31.0	34.0	30.0	34.0
4	35.6339412107586	37.0	35.0	37.0	33.0	37.0
5	35.57223024760415	37.0	35.0	37.0	33.0	37.0
6	35.61408291607381	37.0	35.0	37.0	33.0	37.0
7	35.94777105576615	37.0	35.0	37.0	35.0	37.0
8	34.6055150665986	37.0	35.0	37.0	33.0	37.0
9	36.91390877132006	39.0	37.0	39.0	33.0	39.0
10	36.84296922235859	39.0	37.0	39.0	32.0	39.0
11	36.77843429022436	39.0	37.0	39.0	32.0	39.0
12	36.448797459876104	39.0	35.0	39.0	32.0	39.0
13	36.33441042406601	39.0	35.0	39.0	32.0	39.0
14	37.456107194966094	40.0	35.0	41.0	32.0	41.0
15	37.6256792912562	40.0	35.0	41.0	33.0	41.0
16	37.65257578555214	40.0	35.0	41.0	33.0	41.0
17	37.53235254718381	39.0	35.0	41.0	32.0	41.0
18	37.46540094928803	39.0	36.0	41.0	32.0	41.0
19	37.38043568772696	39.0	35.0	41.0	32.0	41.0
20	37.23798636529849	39.0	35.0	41.0	32.0	41.0
21	37.14422335422346	39.0	35.0	41.0	32.0	41.0
22	37.031119776109946	39.0	35.0	41.0	32.0	41.0
23	36.9670808487837	39.0	35.0	41.0	32.0	41.0
24	36.791133099450775	38.0	35.0	41.0	31.0	41.0
25	36.67625186906921	38.0	35.0	41.0	31.0	41.0
26	36.55132020549805	38.0	35.0	41.0	31.0	41.0
27	36.42993479528035	38.0	35.0	40.0	31.0	41.0
28	36.41990065421948	38.0	35.0	40.0	31.0	41.0
29	36.35912920382177	38.0	35.0	40.0	30.0	41.0
30	36.29932260949216	38.0	35.0	40.0	30.0	41.0
31	36.19931428240414	38.0	35.0	40.0	30.0	41.0
32	36.07505240634741	38.0	35.0	40.0	30.0	41.0
33	35.90517075961145	38.0	35.0	41.0	29.0	41.0
34	35.744615451454884	38.0	35.0	41.0	29.0	41.0
35	35.588717881878445	38.0	35.0	41.0	28.0	41.0
36	35.43060639303131	38.0	35.0	40.0	27.0	41.0
37	35.381040306726476	38.0	34.0	40.0	27.0	41.0
38	35.27459296831724	38.0	34.0	40.0	27.0	41.0
39	35.20384349419095	38.0	34.0	40.0	26.0	41.0
40	35.05263262697904	37.0	34.0	40.0	26.0	41.0
41	34.954794773485105	37.0	34.0	40.0	25.0	41.0
42	34.85274087912327	37.0	34.0	40.0	25.0	41.0
43	34.746117947771054	37.0	34.0	40.0	25.0	41.0
44	34.62268153956996	37.0	34.0	40.0	24.0	41.0
45	34.47789158131401	36.0	34.0	40.0	24.0	41.0
46	34.39398530812036	36.0	34.0	40.0	23.0	41.0
47	34.309244515888444	36.0	33.0	40.0	23.0	41.0
48	34.238817263863695	36.0	33.0	40.0	23.0	41.0
49	34.13820250753964	36.0	33.0	40.0	24.0	41.0
50	34.014454738656156	35.0	33.0	40.0	24.0	41.0
51	33.65862559602038	35.0	33.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	3.0
13	4.0
14	5.0
15	19.0
16	75.0
17	138.0
18	279.0
19	518.0
20	930.0
21	1431.0
22	2144.0
23	3105.0
24	4531.0
25	6332.0
26	8545.0
27	9992.0
28	11007.0
29	11594.0
30	13551.0
31	16142.0
32	20111.0
33	25604.0
34	41790.0
35	62448.0
36	45441.0
37	59074.0
38	81770.0
39	125744.0
40	85.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.04258400402597	23.82325574659585	26.764528053235438	15.36963219614275
2	32.271810634777545	25.069603594405642	28.89445234914394	13.764133421672875
3	29.247267447964752	25.14563352847683	31.647822104436163	13.959276919122251
4	24.9904962582411	28.37093194596806	31.319988269667316	15.318583526123522
5	22.95090276495527	31.625013124214814	30.57797231786305	14.846111792966868
6	20.336921222126882	40.89849279706886	27.92145021668531	10.84313576411894
7	85.81299532596928	4.145984714362778	8.025683635823857	2.0153363238440734
8	83.22652937832858	6.416745411955526	7.62616443464503	2.7305607750708707
9	80.88335921971564	5.1066772384479755	10.319977408248162	3.689986133588215
10	44.33595093534921	24.28577117886223	16.318196135507065	15.060081750281492
11	33.68071772257763	23.101333420224687	24.438555141614803	18.779393715582877
12	29.745263516131015	20.137795204321396	30.326530464470487	19.7904108150771
13	22.26011650682278	27.98589463699327	30.905625128979352	18.8483637272046
14	18.92294547205538	29.679008859297557	31.75100558638992	19.647040082257146
15	18.41553617395649	21.789636033844182	40.28880513527898	19.50602265692035
16	21.11278859695808	19.020336197127516	37.58630302635343	22.28057217956098
17	20.33565405655903	20.575148348883264	29.89279779295963	29.196399801598076
18	23.2086804461871	21.124374110721305	32.50370193369466	23.16324350939694
19	26.33676916225874	24.52689468405942	26.469278475925666	22.667057677756176
20	28.358622337594632	22.45725126445021	26.244447099458014	22.939679298497143
21	24.930396405594355	24.632250449843777	27.836007052681506	22.601346091880366
22	25.01728775881857	22.122719554536996	26.445202330136457	26.414790356507982
23	23.223343362043686	25.330639701383383	25.79623253574312	25.649784400829812
24	22.504317414113327	23.0450350642815	33.10325227094172	21.34739525066345
25	22.834504556365335	23.62648303627352	29.973896389302222	23.56511601805892
26	22.023156545634254	25.891813024289757	27.334028464159125	24.751001965916867
27	21.950566060961524	24.667731085743664	27.917829743634304	25.463873109660508
28	20.976296762935046	24.863960725108342	31.515674838074343	22.64406767388227
29	21.88666471161122	22.720821702563658	31.00283483039894	24.389678755426182
30	23.46790631663933	24.668817227658966	30.06658049940805	21.79669595629365
31	27.100326928716505	24.953567433120813	25.19107046526699	22.755035172895692
32	28.271549960717866	23.672644067673883	25.74989048069021	22.305915490918043
33	25.83406647912616	24.43022805359748	25.22781826673473	24.507887200541624
34	22.891889054223824	24.910664827466356	27.580039607975177	24.617406510334643
35	22.886458344647313	24.67859250489669	29.532017653426596	22.902931497029403
36	27.033529200925393	26.129316056435936	25.2761515819657	21.56100316067297
37	23.7204343119472	27.390869891059964	27.207311907373814	21.681383889619017
38	23.58195121774611	27.72341034079513	26.317399631435844	22.377238810022916
39	22.821289829729153	27.81500830898565	25.44377948422741	23.919922377057787
40	24.634241710021833	24.573417762764883	27.400464144645138	23.391876382568146
41	21.546702292121488	24.1094541412781	27.27791113186849	27.06593243473192
42	24.223136995079777	25.60326132212435	24.763673621595398	25.409928061200475
43	23.141701694743436	24.715340306364432	27.26505845253741	24.877899546354726
44	22.850615661442326	25.766906704029946	27.39268012758547	23.989797506942256
45	21.897526130764245	27.797449014688258	25.928017754799843	24.37700709974765
46	23.838099686104986	26.03789911189796	27.20984623850952	22.91415496348753
47	22.99253820504187	25.90575184553614	27.7947336599	23.306976289521987
48	23.662506743131058	24.374291744959397	28.339795877729383	23.623405634180163
49	22.781464626168056	23.894579065700725	29.323116358383388	24.000839949747835
50	21.731889488680594	26.21276796026169	29.13611892529878	22.919223625758942
51	21.773162881462092	26.654103625179665	27.13653165922659	24.436201834131648
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	631.0
1	864.0
2	1097.0
3	3975.5
4	6854.0
5	4785.5
6	2717.0
7	2709.5
8	2702.0
9	2899.0
10	3096.0
11	3089.5
12	3083.0
13	3114.5
14	3146.0
15	3007.0
16	2868.0
17	2714.5
18	2561.0
19	2370.5
20	2180.0
21	2144.0
22	2108.0
23	2351.0
24	2594.0
25	2640.0
26	3201.0
27	3716.0
28	4330.5
29	4945.0
30	6017.5
31	7090.0
32	8013.0
33	8936.0
34	10661.0
35	12386.0
36	13248.5
37	14111.0
38	15890.5
39	17670.0
40	20331.5
41	22993.0
42	26766.0
43	30539.0
44	32251.5
45	33964.0
46	35696.5
47	37429.0
48	39033.0
49	40637.0
50	40343.5
51	40050.0
52	37669.0
53	35288.0
54	33619.5
55	31951.0
56	31630.5
57	31310.0
58	29710.5
59	28111.0
60	27220.5
61	26330.0
62	24624.5
63	22919.0
64	20810.5
65	18702.0
66	16396.0
67	14090.0
68	12513.5
69	10937.0
70	9459.5
71	7982.0
72	6483.5
73	4985.0
74	4204.5
75	2631.5
76	1839.0
77	1415.0
78	991.0
79	745.0
80	499.0
81	316.5
82	134.0
83	111.0
84	88.0
85	57.5
86	27.0
87	17.0
88	7.0
89	6.0
90	5.0
91	4.0
92	3.0
93	2.5
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	552414.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.562665914810566
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.33254510645193	24.276730461758117
2	6.344357871037751	4.2586712733927765
3	3.0503189658013903	3.071305091484076
4	2.2788655357739733	3.059392105678306
5	1.7921172149733342	3.0074115683165386
6	1.5506674465676364	3.122672007247317
7	1.4504538114790153	3.4076767689673666
8	1.281581390042751	3.4410630429314706
9	1.1699086458683532	3.533872772891516
>10	8.696790600138355	46.24578992245409
>50	0.03220011770902702	0.7345244729276864
>100	0.019101764742641136	1.018992429649972
>500	5.457647069326039E-4	0.10019573234199795
>1k	5.457647069326039E-4	0.7217023499588151
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3940	0.7132331910487425	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.8102365255044225E-4	0.0	0.0	0.04742819696821587	0.0
2	1.8102365255044225E-4	0.0	0.0	0.14771530048116085	0.0
3	1.8102365255044225E-4	0.0	0.0	0.2391322450191342	0.0
4	1.8102365255044225E-4	0.0	0.0	0.39173518411915703	0.0
5	1.8102365255044225E-4	0.0	0.0	0.6368412096724558	0.0
6	1.8102365255044225E-4	0.0	0.0	1.1218035748550905	0.0
7	1.8102365255044225E-4	0.0	0.0	1.3918908644603505	0.0
8	1.8102365255044225E-4	0.0	0.0	2.166853121028794	0.0
9	1.8102365255044225E-4	0.0	0.0	2.552614524613786	0.0
10	1.8102365255044225E-4	0.0	0.0	3.0697990999503997	0.0
11	1.8102365255044225E-4	0.0	0.0	3.3768152146759496	0.0
12	1.8102365255044225E-4	0.0	0.0	3.6756852650367295	0.0
13	1.8102365255044225E-4	0.0	0.0	3.81905599785668	0.0
14	1.8102365255044225E-4	0.0	0.0	3.88042301607128	0.0
15	1.8102365255044225E-4	0.0	0.0	3.9510222405659525	0.0
16	1.8102365255044225E-4	0.0	0.0	4.078824939266565	0.0
17	1.8102365255044225E-4	0.0	0.0	4.21187732389114	0.0
18	1.8102365255044225E-4	0.0	0.0	4.435441534790936	0.0
19	1.8102365255044225E-4	0.0	0.0	4.5375388748293854	0.0
20	1.8102365255044225E-4	0.0	0.0	4.644523853486697	0.0
21	1.8102365255044225E-4	0.0	0.0	4.766714818958245	0.0
22	1.8102365255044225E-4	0.0	0.0	4.891259091912949	0.0
23	1.8102365255044225E-4	0.0	0.0	5.0478445513690815	0.0
24	1.8102365255044225E-4	0.0	0.0	5.175104179112043	0.0
25	1.8102365255044225E-4	0.0	0.0	5.280821992201501	0.0
26	1.8102365255044225E-4	0.0	0.0	5.379117835536391	0.0
27	1.8102365255044225E-4	0.0	0.0	5.494429902211023	0.0
28	1.8102365255044225E-4	0.0	0.0	5.595079053029068	0.0
29	1.8102365255044225E-4	0.0	0.0	5.706770646652692	0.0
30	1.8102365255044225E-4	0.0	0.0	5.87801902196541	0.0
31	3.620473051008845E-4	0.0	0.0	5.992244946724739	0.0
32	3.620473051008845E-4	0.0	0.0	6.125840402306966	0.0
33	3.620473051008845E-4	0.0	0.0	6.240066327066295	0.0
34	3.620473051008845E-4	0.0	0.0	6.363705481758246	0.0
35	3.620473051008845E-4	0.0	0.0	6.521015035824581	0.0
36	3.620473051008845E-4	0.0	0.0	6.646464427042037	0.0
37	3.620473051008845E-4	0.0	0.0	6.777525551488558	0.0
38	3.620473051008845E-4	0.0	0.0	6.910577936113133	0.0
39	3.620473051008845E-4	0.0	0.0	7.047974888398918	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCACGT	35	1.2103919E-7	45.000004	45
TCCGCAA	20	7.0301007E-4	45.000004	32
ACGTTAG	20	7.0301007E-4	45.000004	1
CACGACG	35	1.2103919E-7	45.000004	26
GTTACAC	50	2.1827873E-11	45.000004	33
AATGCGT	20	7.0301007E-4	45.000004	29
TACACGG	20	7.0301007E-4	45.000004	2
CCGCAAA	30	2.163446E-6	45.000004	41
TGATTCG	65	0.0	41.53846	15
TAGGGCG	290	0.0	41.12069	5
CGTTTTT	2480	0.0	41.008064	1
CGGGTAT	50	1.0786607E-9	40.500004	6
TTTACGG	45	1.9252184E-8	40.0	2
TAACGCC	45	1.9252184E-8	40.0	12
ACGGGAT	90	0.0	40.0	5
ATAACGC	45	1.9252184E-8	40.0	11
CGCATCG	40	3.453879E-7	39.375004	21
CGGTCTA	40	3.453879E-7	39.375004	31
CGTTTAT	40	3.453879E-7	39.375004	39
ATAGGGC	275	0.0	39.272728	4
>>END_MODULE