FastQCFastQC Report
Thu 26 May 2016
SRR1546561_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546561_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences533869
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98401.8431487874366184No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTT5480.10264690401577915No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATGCG207.0298876E-445.0000041
TCGTCAG253.88799E-544.9999961
TATCGCG302.1633095E-644.9999961
AGTAACG302.1633095E-644.9999961
CTAGCGG502.1827873E-1144.9999962
ACGGGCT1350.043.3333325
CGTTTTT62150.043.2260671
CACAACG1050.042.85714312
CGACGGT700.041.78571328
TCACGAC650.041.5384625
CTCACGA650.041.5384624
CGGTCTA650.041.5384631
ACAACGA1100.040.90909213
ACGGGAT1000.040.4999965
ATCGTTG501.0786607E-940.49999623
TGATTCG1000.040.49999615
TAATCGT501.0786607E-940.49999621
GCGATGT1550.039.193559
CACGACG700.038.57142626
GCGATCA356.242004E-638.5714269
Produced by FastQC (version 0.11.5)