##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546561_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	533869
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.414202735127905	31.0	31.0	33.0	30.0	34.0
2	31.77005407693648	31.0	31.0	34.0	30.0	34.0
3	31.751334128784404	31.0	31.0	34.0	30.0	34.0
4	35.60801807184909	37.0	35.0	37.0	33.0	37.0
5	35.56566123899309	37.0	35.0	37.0	33.0	37.0
6	35.62320344503989	37.0	35.0	37.0	33.0	37.0
7	35.97254569941315	37.0	35.0	37.0	35.0	37.0
8	34.64647694471865	37.0	35.0	37.0	33.0	37.0
9	36.91906066844113	39.0	37.0	39.0	33.0	39.0
10	36.891743105518394	39.0	37.0	39.0	32.0	39.0
11	36.84145361502541	39.0	37.0	39.0	32.0	39.0
12	36.60043568740646	39.0	35.0	39.0	32.0	39.0
13	36.552674907140144	39.0	35.0	39.0	32.0	39.0
14	37.66417417006794	40.0	36.0	41.0	32.0	41.0
15	37.777289934422114	40.0	36.0	41.0	33.0	41.0
16	37.79114539334556	40.0	36.0	41.0	33.0	41.0
17	37.651500649035626	40.0	36.0	41.0	32.0	41.0
18	37.489814917142596	39.0	36.0	41.0	32.0	41.0
19	37.30427314565933	39.0	36.0	41.0	32.0	41.0
20	37.04834893953385	39.0	35.0	41.0	32.0	41.0
21	36.95683023363409	38.0	35.0	41.0	32.0	41.0
22	36.871644542013115	38.0	35.0	41.0	32.0	41.0
23	36.81051718680051	38.0	35.0	41.0	31.0	41.0
24	36.66607913177203	38.0	35.0	41.0	31.0	41.0
25	36.54938009137073	38.0	35.0	40.0	31.0	41.0
26	36.465232107502025	38.0	35.0	40.0	31.0	41.0
27	36.346053057959914	38.0	35.0	40.0	31.0	41.0
28	36.30694046666879	38.0	35.0	40.0	31.0	41.0
29	36.19554422526875	38.0	35.0	40.0	30.0	41.0
30	36.059833030200295	38.0	35.0	40.0	30.0	41.0
31	35.85855706175111	38.0	35.0	40.0	30.0	41.0
32	35.58212969848409	38.0	35.0	40.0	27.0	41.0
33	35.19029949294677	38.0	34.0	40.0	25.0	41.0
34	34.764865538175094	38.0	34.0	40.0	23.0	41.0
35	34.507716312428705	38.0	34.0	40.0	21.0	41.0
36	34.32708585814123	38.0	34.0	40.0	20.0	41.0
37	34.28447428114388	38.0	34.0	40.0	20.0	41.0
38	34.148813660279956	38.0	33.0	40.0	18.0	41.0
39	34.05344382236092	37.0	33.0	40.0	18.0	41.0
40	33.89324160046753	37.0	33.0	40.0	18.0	41.0
41	33.7703406640955	37.0	33.0	40.0	18.0	41.0
42	33.66741279227676	37.0	33.0	40.0	18.0	41.0
43	33.555336608793546	37.0	33.0	40.0	17.0	41.0
44	33.415999055948184	36.0	33.0	40.0	17.0	41.0
45	33.293703136911866	36.0	33.0	40.0	17.0	41.0
46	33.22418233686541	36.0	33.0	40.0	17.0	41.0
47	33.17011851221929	36.0	33.0	40.0	17.0	41.0
48	33.09234662435916	35.0	32.0	40.0	17.0	41.0
49	33.01063182166411	35.0	32.0	40.0	17.0	41.0
50	32.91032256976899	35.0	32.0	40.0	17.0	41.0
51	32.55975154953743	35.0	31.0	39.0	15.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	0.0
11	2.0
12	0.0
13	4.0
14	12.0
15	36.0
16	60.0
17	142.0
18	317.0
19	660.0
20	1147.0
21	1788.0
22	2595.0
23	3875.0
24	5849.0
25	9087.0
26	13024.0
27	15089.0
28	14748.0
29	14041.0
30	14517.0
31	15892.0
32	18867.0
33	23628.0
34	36678.0
35	51835.0
36	41916.0
37	52829.0
38	75059.0
39	120067.0
40	103.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.218203342018356	22.03911446440981	24.236844619185604	21.505837574386224
2	39.21973367998516	22.824887753362717	24.9358925129573	13.01948605369482
3	27.835480239534416	23.067831246991304	35.828077674485684	13.26861083898859
4	24.250518385596468	25.765684091041063	35.24310270871693	14.740694814645542
5	21.301480325697877	28.988572102894157	34.896763063597994	14.813184507809968
6	19.61136533494172	37.38033112992139	32.80879766384637	10.199505871290523
7	79.49197274986936	3.5113482895616714	14.925009693389201	2.071669267179776
8	77.01496059894843	5.9486503243305	14.325424401866377	2.7109646748546927
9	74.27009247586955	4.767461680674472	17.122365224427718	3.8400806190282633
10	36.46793501776653	25.930518535445962	23.377270453987776	14.22427599279973
11	29.57560749921797	23.93208820890518	30.65077762522267	15.841526666654179
12	27.004752102107442	20.534812847346444	34.33763713570183	18.122797914844277
13	23.519814786024288	23.61628039837488	34.979742221406376	17.884162594194457
14	19.67636255336047	26.23396376264589	36.15381301405401	17.93586066993963
15	19.387340340045967	22.12921147322658	40.575684297084116	17.907763889643338
16	22.663050298856085	20.36960377920426	38.696009695262326	18.27133622667733
17	21.052542852272747	21.131588460839644	35.71400474648275	22.101863940404858
18	23.10079813587228	21.52082252387758	36.120471501435745	19.257907838814393
19	25.343483139122142	25.02505296243086	31.18499107458946	18.446472823857537
20	26.43551133330461	24.219237303533266	30.68561763279007	18.659633730372057
21	24.398307449954952	25.1029746997859	32.95115468401424	17.547563166244903
22	22.858791201586907	21.77650322457382	33.0300129807125	22.33469259312678
23	21.128029535335447	25.284292588631292	32.19235430414577	21.395323571887488
24	21.839252700568867	23.482539724164543	35.88651897750197	18.79168859776462
25	23.06539619269896	23.415856698928014	32.9777529693614	20.54099413901163
26	19.79380709499896	25.026738769248634	32.61923805278074	22.560216082971664
27	19.801112257875996	23.220115796197195	33.94297102847328	23.035800917453532
28	19.813474841206364	25.32344076917746	35.013832981499206	19.84925140811697
29	21.679101052880014	24.001580912171338	34.05816782768807	20.261150207260584
30	22.985414024788852	23.193704822718683	34.91268457243256	18.9081965800599
31	25.08499276039628	24.210620957575735	30.995618775392465	19.70876750663552
32	25.318196036855483	24.136258145724888	30.70210107723056	19.843444740189074
33	23.942952297286414	24.510132635534184	29.99593533245047	21.55097973472893
34	20.950083260125613	26.81818948094008	32.01440802893594	20.21731922999837
35	20.405754969852154	27.07480674097953	31.863060039073254	20.656378250095063
36	22.82207807533309	28.658903214084354	27.73807806784061	20.780940642741946
37	21.95182713362267	28.20673236318273	28.81156238702753	21.029878116167076
38	22.450076704210208	28.704794621901623	27.539340175211525	21.30578849867664
39	22.304160758538146	26.696062142585543	27.25706118916813	23.74271590970819
40	23.51925285041836	24.843547761716824	29.113883743015606	22.523315644849205
41	20.917678306850558	24.760755915777093	29.723209251707814	24.598356525664535
42	22.741533971817056	26.61252104917124	27.397732402518223	23.248212576493486
43	21.61653888875361	25.44575541940064	29.337908737911363	23.599796953934394
44	22.483605528697115	25.73796193448206	29.090095135698085	22.688337401122745
45	21.463692403941792	26.226471287900218	29.4984350093375	22.811401298820495
46	22.560216082971664	26.349909809335248	28.807441525917405	22.28243258177568
47	21.36685216785391	27.08192459198792	29.79925787037644	21.751965369781725
48	21.70007998216791	25.70949053044848	30.706971185815245	21.88345830156836
49	22.394070455486272	24.792973557183505	30.161331712461298	22.65162427486893
50	21.1930267537542	25.131820727556757	31.059492122599362	22.615660396089677
51	20.872910770245134	26.208302036641946	30.152902678372413	22.76588451474051
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	568.0
1	840.0
2	1112.0
3	8947.0
4	16782.0
5	11546.5
6	6311.0
7	6178.0
8	6045.0
9	6089.5
10	6134.0
11	6064.5
12	5995.0
13	5782.0
14	5569.0
15	5358.0
16	5147.0
17	4828.0
18	4509.0
19	4203.0
20	3897.0
21	3782.5
22	3668.0
23	3596.5
24	3525.0
25	3691.0
26	4152.5
27	4448.0
28	5053.0
29	5658.0
30	6208.0
31	6758.0
32	7770.5
33	8783.0
34	10118.0
35	11453.0
36	12596.5
37	13740.0
38	15011.0
39	16282.0
40	18830.5
41	21379.0
42	23730.0
43	26081.0
44	28136.0
45	30191.0
46	33294.5
47	36398.0
48	37806.0
49	39214.0
50	38726.5
51	38239.0
52	35839.0
53	33439.0
54	31502.5
55	29566.0
56	28417.5
57	27269.0
58	25948.5
59	24628.0
60	23275.5
61	21923.0
62	20134.5
63	18346.0
64	16333.5
65	14321.0
66	12062.5
67	9804.0
68	8377.0
69	6950.0
70	6269.0
71	5588.0
72	4844.5
73	4101.0
74	3495.5
75	2247.0
76	1604.0
77	1209.0
78	814.0
79	639.5
80	465.0
81	339.0
82	213.0
83	165.0
84	117.0
85	83.0
86	49.0
87	35.5
88	22.0
89	15.5
90	9.0
91	6.0
92	3.0
93	3.5
94	4.0
95	2.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	533869.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.143579376825095
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.51332005215161	32.299631422484325
2	10.275684992195218	9.688651421616115
3	6.331206828245521	8.954272549754695
4	4.54046476522612	8.562150442684606
5	3.186776394639029	7.5118022958428785
6	2.183829253058513	6.177211664219801
7	1.5399096227330418	5.081779607271637
8	1.075303732544092	4.055493349551097
9	0.7147785064867808	3.0327495531667235
>10	1.5942919962423114	10.198770910215451
>50	0.02342876077795813	0.7577358167527835
>100	0.020197207403708773	1.701525378312949
>500	4.039441480741753E-4	0.10435768408679305
>1k	0.0	0.0
>5k	4.039441480741753E-4	1.8738679040402255
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9840	1.8431487874366184	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTT	548	0.10264690401577915	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.8731186864193275E-4	0.0	0.0	0.042332482313076805	0.0
2	1.8731186864193275E-4	0.0	0.0	0.12624819946466267	0.0
3	1.8731186864193275E-4	0.0	0.0	0.24912478529377058	0.0
4	1.8731186864193275E-4	0.0	0.0	0.3697536286991753	0.0
5	1.8731186864193275E-4	0.0	0.0	0.6222500276285007	0.0
6	1.8731186864193275E-4	0.0	0.0	1.3016301751927908	0.0
7	1.8731186864193275E-4	0.0	0.0	1.7142782218109687	0.0
8	1.8731186864193275E-4	0.0	0.0	2.5586801256488014	0.0
9	1.8731186864193275E-4	0.0	0.0	3.1412200371252124	0.0
10	1.8731186864193275E-4	0.0	0.0	3.894588372803066	0.0
11	1.8731186864193275E-4	0.0	0.0	4.319786314620253	0.0
12	1.8731186864193275E-4	0.0	0.0	4.6348448776759845	0.0
13	1.8731186864193275E-4	0.0	0.0	4.803612871322366	0.0
14	1.8731186864193275E-4	0.0	0.0	4.908882141499132	0.0
15	1.8731186864193275E-4	0.0	0.0	4.9853053839050405	0.0
16	1.8731186864193275E-4	0.0	0.0	5.091698525293658	0.0
17	1.8731186864193275E-4	0.0	0.0	5.2050222058220275	0.0
18	1.8731186864193275E-4	0.0	0.0	5.404696657794328	0.0
19	1.8731186864193275E-4	0.0	0.0	5.489736246157765	0.0
20	1.8731186864193275E-4	0.0	0.0	5.597440570626877	0.0
21	1.8731186864193275E-4	0.0	0.0	5.70439564762142	0.0
22	1.8731186864193275E-4	0.0	0.0	5.815096961988803	0.0
23	1.8731186864193275E-4	0.0	0.0	5.947713764987291	0.0
24	1.8731186864193275E-4	0.0	0.0	6.065532930363067	0.0
25	1.8731186864193275E-4	0.0	0.0	6.158064993472181	0.0
26	1.8731186864193275E-4	0.0	0.0	6.249285873500803	0.0
27	1.8731186864193275E-4	0.0	0.0	6.364669984584233	0.0
28	1.8731186864193275E-4	0.0	0.0	6.461510220672112	0.0
29	1.8731186864193275E-4	0.0	0.0	6.580827880997024	0.0
30	1.8731186864193275E-4	0.0	0.0	6.737045979444395	0.0
31	1.8731186864193275E-4	0.0	0.0	6.852055466790542	0.0
32	1.8731186864193275E-4	0.0	0.0	6.985796141000883	0.0
33	1.8731186864193275E-4	0.0	0.0	7.1156032659697415	0.0
34	1.8731186864193275E-4	0.0	0.0	7.239978346747985	0.0
35	1.8731186864193275E-4	0.0	0.0	7.384208485602273	0.0
36	1.8731186864193275E-4	0.0	0.0	7.527876688850635	0.0
37	1.8731186864193275E-4	0.0	0.0	7.678100807501465	0.0
38	1.8731186864193275E-4	0.0	0.0	7.870657408465372	0.0
39	1.8731186864193275E-4	0.0	0.0	8.096555522047543	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAATGCG	20	7.0298876E-4	45.000004	1
TCGTCAG	25	3.88799E-5	44.999996	1
TATCGCG	30	2.1633095E-6	44.999996	1
AGTAACG	30	2.1633095E-6	44.999996	1
CTAGCGG	50	2.1827873E-11	44.999996	2
ACGGGCT	135	0.0	43.333332	5
CGTTTTT	6215	0.0	43.226067	1
CACAACG	105	0.0	42.857143	12
CGACGGT	70	0.0	41.785713	28
TCACGAC	65	0.0	41.53846	25
CTCACGA	65	0.0	41.53846	24
CGGTCTA	65	0.0	41.53846	31
ACAACGA	110	0.0	40.909092	13
ACGGGAT	100	0.0	40.499996	5
ATCGTTG	50	1.0786607E-9	40.499996	23
TGATTCG	100	0.0	40.499996	15
TAATCGT	50	1.0786607E-9	40.499996	21
GCGATGT	155	0.0	39.19355	9
CACGACG	70	0.0	38.571426	26
GCGATCA	35	6.242004E-6	38.571426	9
>>END_MODULE