FastQCFastQC Report
Thu 26 May 2016
SRR1546560_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546560_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2396654
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT139420.5817276920239635No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC50510.21075215696550273No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA48900.2040344580402511No Hit
CTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTGCT31400.13101599146142914TruSeq Adapter, Index 21 (95% over 23bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT25930.10819250505079164No Hit
GAGCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCT25270.10543866573981893No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAATCG302.1664928E-645.0000041
CTACGTC302.1664928E-645.00000426
ACCGTTC302.1664928E-645.00000423
TAATGCG302.1664928E-645.0000041
TACACGG1350.045.0000042
CTTAACG406.8212103E-945.01
CTCCGAT253.8920785E-545.035
ACGTTAC253.8920785E-545.016
ATAGCCG700.045.014
TGCGAAC207.0348213E-445.033
GTCGTAC351.2126657E-745.027
ACGTCGT207.0348213E-445.020
CGGTCTA6350.044.64566831
CGCATCG2250.044.021
CACGACG6550.042.5954226
TTGTGCG1800.042.5000041
AAATGCG750.042.0000041
CGACGGT6700.041.9776128
ACGGTCT6800.041.69117730
TCTCACG6750.041.6666723
Produced by FastQC (version 0.11.5)