FastQCFastQC Report
Thu 26 May 2016
SRR1546559_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546559_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2908935
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT711222.4449497840274876No Hit
CGTTTCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTT111670.3838861989009723No Hit
CGTTCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTC79250.27243647589237985No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCT78680.27047699587649776No Hit
CGTCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCT42080.14465775275143652No Hit
CGCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTG38510.13238522002038547TruSeq Adapter, Index 14 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGC38510.13238522002038547TruSeq Adapter, Index 15 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGCT35060.12052520939794117TruSeq Adapter, Index 14 (95% over 23bp)
CGTTTTTCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTC31920.10973088088939767No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT543200.044.217141
GTTTTTT589350.039.5940482
TACGGGA6900.038.8043484
ACGCCGA1400.038.57142612
CGTTCTG9950.038.442211
CGTTTTC11300.038.4292031
CGTTTCT15050.038.2724271
AGGGCGA26050.038.2629556
CGCATCG1000.038.25000421
CGCGTAG301.1403516E-437.5000041
TAGGGCG11950.037.468625
CGTAAGG3500.037.2857132
ACCGCTC1400.036.96428318
TGGGCGA15850.036.908526
GGCACCG12050.036.597518
CGACGGT1600.036.56250428
TACGCGG1300.036.3461532
TTCGCGG1300.036.3461532
TAGGGTA20400.036.2867665
AAGGGCG11050.036.2443475
Produced by FastQC (version 0.11.5)