##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546559_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2908935
Sequences flagged as poor quality	0
Sequence length	51
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.366219939599887	31.0	31.0	33.0	30.0	34.0
2	31.712884612409695	31.0	31.0	34.0	30.0	34.0
3	31.524587520862447	31.0	31.0	34.0	30.0	34.0
4	35.49025639967892	37.0	35.0	37.0	33.0	37.0
5	35.4772849857422	37.0	35.0	37.0	33.0	37.0
6	35.546024919773046	37.0	35.0	37.0	33.0	37.0
7	36.00401143373778	37.0	35.0	37.0	35.0	37.0
8	34.7082499265195	37.0	35.0	37.0	33.0	37.0
9	36.99110705464371	39.0	37.0	39.0	33.0	39.0
10	36.79011803288832	39.0	37.0	39.0	32.0	39.0
11	36.805460417644255	39.0	37.0	39.0	32.0	39.0
12	36.800097286463945	39.0	35.0	39.0	32.0	39.0
13	36.85410364961747	39.0	37.0	39.0	33.0	39.0
14	38.07820628511809	40.0	37.0	41.0	33.0	41.0
15	38.172269576322606	40.0	37.0	41.0	33.0	41.0
16	38.150551662378156	40.0	37.0	41.0	33.0	41.0
17	38.051416068079895	40.0	37.0	41.0	33.0	41.0
18	37.751918141862916	39.0	37.0	41.0	33.0	41.0
19	37.45786275733215	38.0	36.0	41.0	33.0	41.0
20	37.07598897878433	38.0	35.0	41.0	32.0	41.0
21	37.00739239618623	38.0	35.0	41.0	32.0	41.0
22	36.916755444862126	38.0	35.0	41.0	32.0	41.0
23	36.827943216331754	38.0	35.0	40.0	32.0	41.0
24	36.65589021411616	38.0	35.0	40.0	32.0	41.0
25	36.55962818007278	38.0	35.0	40.0	31.0	41.0
26	36.45346114643332	38.0	35.0	40.0	31.0	41.0
27	36.32028354019598	38.0	35.0	40.0	31.0	41.0
28	36.23091200044002	38.0	35.0	40.0	31.0	41.0
29	36.08912540156449	38.0	35.0	40.0	30.0	41.0
30	35.86654222249724	38.0	35.0	40.0	30.0	41.0
31	35.581399721891344	38.0	35.0	40.0	29.0	41.0
32	35.00680902117098	38.0	34.0	40.0	24.0	41.0
33	34.3408329852678	38.0	33.0	40.0	20.0	41.0
34	33.67632862198708	38.0	33.0	40.0	15.0	41.0
35	33.21974399565477	38.0	33.0	40.0	11.0	41.0
36	32.95717195468445	38.0	32.0	40.0	10.0	41.0
37	32.86867771194613	38.0	32.0	40.0	10.0	41.0
38	32.753187678652154	38.0	32.0	40.0	10.0	41.0
39	32.668498608597304	38.0	31.0	40.0	10.0	41.0
40	32.57690804366546	37.0	31.0	40.0	10.0	41.0
41	32.47996844205869	37.0	31.0	40.0	9.0	41.0
42	32.38127905917458	37.0	31.0	40.0	8.0	41.0
43	32.30353273620758	37.0	31.0	40.0	8.0	41.0
44	32.1885256975491	37.0	31.0	40.0	8.0	41.0
45	32.10627841460878	37.0	30.0	40.0	8.0	41.0
46	32.02817869770208	37.0	30.0	40.0	8.0	41.0
47	31.956003485811816	37.0	30.0	40.0	8.0	41.0
48	31.834263055035606	36.0	30.0	40.0	8.0	41.0
49	31.756947817672103	36.0	30.0	40.0	8.0	41.0
50	31.668489670618285	36.0	29.0	40.0	8.0	41.0
51	31.347535781995816	35.0	28.0	40.0	8.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	3.0
11	2.0
12	9.0
13	13.0
14	21.0
15	89.0
16	314.0
17	837.0
18	2005.0
19	3957.0
20	7209.0
21	11621.0
22	18017.0
23	29062.0
24	47747.0
25	82649.0
26	116600.0
27	112109.0
28	88117.0
29	74009.0
30	73475.0
31	82017.0
32	95740.0
33	117308.0
34	162384.0
35	230658.0
36	228829.0
37	271928.0
38	384100.0
39	667660.0
40	444.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.3014694381277	21.229831536283896	23.56914128366567	27.89955774192273
2	43.75498249359301	21.484838953087642	23.71001758375488	11.050160969564462
3	23.354767294559693	21.409966190375513	43.4220083982626	11.813258116802198
4	20.348959327038934	23.8271738625992	43.53902716973737	12.28483964062449
5	17.970528733024285	27.56816498134197	42.52539159520581	11.93591469042794
6	15.290406970248563	35.32210241892651	39.93031814048784	9.457172470337081
7	72.71544396832518	2.5558838544003217	22.521610142543576	2.207062034730924
8	70.43010586348612	5.117921163587361	21.344650189846114	3.1073227830804058
9	67.11559385135797	4.436675278065684	24.657408983012683	3.7903218875636613
10	28.005885315416123	22.325455879901064	34.540854298910084	15.127804505772732
11	22.263818201506737	25.465814808512395	36.71549897127299	15.554868018707877
12	18.070737228573343	21.103737278419764	42.70611065561795	18.11941483738894
13	17.907790995673675	23.31406511317716	43.93577030768993	14.84237358345924
14	16.469017011380455	26.55305120258789	41.86903454357007	15.10889724246159
15	15.431042632441084	24.782987588241056	45.53058077956366	14.255388999754206
16	16.15030930563935	23.625209913593807	44.44365377706961	15.78082700369723
17	16.14291828452681	25.01874397330982	40.733533062787586	18.104804679375786
18	17.265081550464345	24.55249086005703	41.51000280171265	16.67242478776597
19	18.743698295080502	27.63946255244617	37.70933348459144	15.907505667881889
20	19.02373205313972	25.066080885272445	38.28459556504357	17.625591496544267
21	18.120480519502845	26.952475734246384	39.092382607380365	15.834661138870413
22	17.432943671824912	24.427427907464416	38.79134459862458	19.348283822086092
23	15.882341819256876	27.12009721771026	38.15166031554504	18.845900647487827
24	16.099053433644958	25.69950858303812	41.074173193969614	17.127264789347304
25	17.235173697590355	26.580896444918846	39.14051018671782	17.04341967077298
26	16.085887103011927	27.715882273065574	38.811042529310555	17.387188094611947
27	17.327441142548732	26.286630674112693	37.823636485517895	18.56229169782068
28	17.436037587639465	26.06049980491142	39.717044210338145	16.78641839711097
29	17.354255079608173	25.347902239135628	38.15967011982048	19.138172561435717
30	17.172985989717887	26.605063365114724	37.71648386780729	18.5054667773601
31	17.861794780564022	25.653615498455622	36.089393540935085	20.395196180045275
32	17.626794686027704	26.120453018028932	36.27939434879088	19.973357947152483
33	17.228195198586423	25.87407418866355	35.104015730843074	21.793714881906954
34	16.555852915242177	26.600078722969062	34.136720139844996	22.70734822194377
35	18.216254402384376	25.583486739992473	33.881918984095556	22.318339873527595
36	19.114693178087514	26.547379023594548	32.38339804773912	21.954529750578818
37	18.708187016897938	27.53416628422429	34.032248915840334	19.725397783037437
38	18.324988354844642	28.388018295355515	33.175990525742236	20.111002824057604
39	19.16866482063023	27.59033804467958	32.36414701600414	20.87685011868605
40	19.584418352421075	27.218930639563965	33.08396371868055	20.112687289334414
41	18.40481138286005	26.910089087587036	32.78715406153799	21.897945468014925
42	19.430650736437908	29.036949949036334	31.358108723639404	20.174290590886354
43	19.07646612935662	28.17381619046146	32.52812455417533	20.221593126006596
44	20.079788651173025	29.253833447636335	30.51089144308828	20.155486458102363
45	18.712999774831683	29.057026024988524	31.147172418771817	21.082801781407973
46	20.753196616631172	28.584035050628493	29.271331260409738	21.391437072330596
47	20.234965717693935	28.021733039755098	30.550046666563535	21.19325457598743
48	19.529552911976374	27.75115291335145	30.983057373231098	21.736236801441077
49	19.410334022589023	26.757043385293933	31.15229456828702	22.680328023830025
50	18.886809089924665	26.89967290434472	32.53348734158721	21.680030664143406
51	18.332620013853866	27.658060424175858	31.294580318913965	22.714739243056307
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2440.0
1	2889.5
2	3339.0
3	52053.0
4	100767.0
5	69533.5
6	38300.0
7	39557.0
8	40814.0
9	44640.0
10	48466.0
11	51566.0
12	54666.0
13	56323.5
14	57981.0
15	57259.5
16	56538.0
17	54706.5
18	52875.0
19	50067.5
20	47260.0
21	44990.5
22	42721.0
23	41434.5
24	40148.0
25	39201.5
26	38853.0
27	39451.0
28	41032.0
29	42613.0
30	45179.5
31	47746.0
32	53433.5
33	59121.0
34	67006.5
35	74892.0
36	82332.5
37	89773.0
38	99603.0
39	109433.0
40	121770.0
41	134107.0
42	150733.5
43	167360.0
44	186138.5
45	204917.0
46	236154.0
47	267391.0
48	268655.5
49	269920.0
50	246017.0
51	222114.0
52	191165.0
53	160216.0
54	139351.5
55	118487.0
56	103526.0
57	88565.0
58	75713.0
59	62861.0
60	53971.5
61	45082.0
62	37494.5
63	29907.0
64	25103.0
65	20299.0
66	15889.5
67	11480.0
68	9190.0
69	6900.0
70	5805.0
71	4710.0
72	3909.0
73	3108.0
74	2430.5
75	1358.5
76	964.0
77	744.0
78	524.0
79	407.0
80	290.0
81	231.5
82	173.0
83	139.5
84	106.0
85	77.0
86	48.0
87	40.5
88	33.0
89	20.5
90	8.0
91	7.0
92	6.0
93	5.5
94	5.0
95	3.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2908935.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.12007572335335
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.36773041353982	30.99126781702039
2	8.36792319636708	6.881792709636388
3	4.212877541909972	5.197015306096583
4	2.5807341154490917	4.244799289964319
5	1.744555326513359	3.586812356490438
6	1.2457369796087507	3.073487935973599
7	0.9756385393739153	2.8082831432374076
8	0.7794498475192058	2.564082940203677
9	0.6071743911044635	2.247035124554685
>10	3.9880416915174264	28.511027430563434
>50	0.09590737243384581	2.609741962556514
>100	0.030816906405912184	2.153613293114052
>500	0.0017921810850458411	0.4742424384372697
>1k	0.0012801293464349322	1.2148382961037711
>5k	1.7068391285799097E-4	0.5542186495570761
>10k+	1.7068391285799097E-4	2.8877413064903585
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	71122	2.4449497840274876	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTT	11167	0.3838861989009723	No Hit
CGTTCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTC	7925	0.27243647589237985	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCT	7868	0.27047699587649776	No Hit
CGTCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCT	4208	0.14465775275143652	No Hit
CGCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTG	3851	0.13238522002038547	TruSeq Adapter, Index 14 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGC	3851	0.13238522002038547	TruSeq Adapter, Index 15 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGCT	3506	0.12052520939794117	TruSeq Adapter, Index 14 (95% over 23bp)
CGTTTTTCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTC	3192	0.10973088088939767	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.13039136316211947	0.0
2	0.0	0.0	0.0	0.4143440812531047	0.0
3	0.0	0.0	0.0	0.7405803154762826	0.0
4	0.0	0.0	0.0	1.0920835288516244	0.0
5	0.0	0.0	0.0	1.8822008742031018	0.0
6	0.0	0.0	0.0	3.457004023809401	0.0
7	0.0	0.0	0.0	4.507079051267904	0.0
8	3.4376842383896515E-5	0.0	0.0	6.1955320417953645	0.0
9	3.4376842383896515E-5	0.0	0.0	7.245194547145261	0.0
10	3.4376842383896515E-5	0.0	0.0	8.55753050515051	0.0
11	3.4376842383896515E-5	0.0	0.0	9.20553398408696	0.0
12	3.4376842383896515E-5	0.0	0.0	9.90709658345752	0.0
13	3.4376842383896515E-5	0.0	0.0	10.390710002114176	0.0
14	3.4376842383896515E-5	0.0	0.0	10.626466387182939	0.0
15	3.4376842383896515E-5	0.0	0.0	10.809007420241429	0.0
16	3.4376842383896515E-5	0.0	0.0	11.076631138200064	0.0
17	3.4376842383896515E-5	0.0	0.0	11.34463300142492	0.0
18	3.4376842383896515E-5	0.0	0.0	11.853169630809901	0.0
19	3.4376842383896515E-5	0.0	0.0	12.041451596546503	0.0
20	3.4376842383896515E-5	0.0	0.0	12.264488549933223	0.0
21	3.4376842383896515E-5	0.0	0.0	12.468240094742578	0.0
22	3.4376842383896515E-5	0.0	0.0	12.685398608081652	0.0
23	3.4376842383896515E-5	0.0	0.0	12.934974483788741	0.0
24	3.4376842383896515E-5	0.0	0.0	13.156980131903945	0.0
25	3.4376842383896515E-5	0.0	0.0	13.34945607241138	0.0
26	3.4376842383896515E-5	0.0	0.0	13.532994033899005	0.0
27	3.4376842383896515E-5	0.0	0.0	13.752524549362567	0.0
28	3.4376842383896515E-5	0.0	0.0	13.951566466765328	0.0
29	3.4376842383896515E-5	0.0	0.0	14.169790662218304	0.0
30	3.4376842383896515E-5	0.0	0.0	14.486917033209748	0.0
31	3.4376842383896515E-5	0.0	0.0	14.705760011825634	0.0
32	3.4376842383896515E-5	0.0	0.0	14.921955973577958	0.0
33	3.4376842383896515E-5	3.4376842383896515E-5	0.0	15.142002141677281	0.0
34	3.4376842383896515E-5	3.4376842383896515E-5	0.0	15.364798457167314	0.0
35	3.4376842383896515E-5	3.4376842383896515E-5	0.0	15.596463997992393	0.0
36	3.4376842383896515E-5	6.875368476779303E-5	0.0	15.816338281879794	0.0
37	3.4376842383896515E-5	6.875368476779303E-5	0.0	16.04683501006382	0.0
38	3.4376842383896515E-5	6.875368476779303E-5	0.0	16.28774792149017	0.0
39	3.4376842383896515E-5	6.875368476779303E-5	0.0	16.52800767291122	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	54320	0.0	44.21714	1
GTTTTTT	58935	0.0	39.594048	2
TACGGGA	690	0.0	38.804348	4
ACGCCGA	140	0.0	38.571426	12
CGTTCTG	995	0.0	38.44221	1
CGTTTTC	1130	0.0	38.429203	1
CGTTTCT	1505	0.0	38.272427	1
AGGGCGA	2605	0.0	38.262955	6
CGCATCG	100	0.0	38.250004	21
CGCGTAG	30	1.1403516E-4	37.500004	1
TAGGGCG	1195	0.0	37.46862	5
CGTAAGG	350	0.0	37.285713	2
ACCGCTC	140	0.0	36.964283	18
TGGGCGA	1585	0.0	36.90852	6
GGCACCG	1205	0.0	36.59751	8
CGACGGT	160	0.0	36.562504	28
TACGCGG	130	0.0	36.346153	2
TTCGCGG	130	0.0	36.346153	2
TAGGGTA	2040	0.0	36.286766	5
AAGGGCG	1105	0.0	36.244347	5
>>END_MODULE