FastQCFastQC Report
Thu 26 May 2016
SRR1546558_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546558_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1902352
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT177110.9310054080422551No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC22830.12000933581166892No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTCGA900.042.50000414
CGTTTTT101250.041.3333321
AACCCGA1050.038.5714324
GTACGAT356.250446E-638.5714322
ATTGGGC5800.038.405174
TCACGAC3000.038.25000425
CGGTCTA3000.038.25000431
CGCATCG1650.038.18181621
TATAGCG1650.038.1818161
TAGCATA15100.038.14569530
CGACGGT2900.038.0172428
CGCCAGT1600.037.96875426
GGGCGAT42350.037.5619857
ATAATCG301.1402031E-437.5000041
GCGTAAG1450.037.241381
AGGGTAC9400.037.1010636
CATATGC15550.037.04179833
CAGTAGC16300.036.99386627
CGTAAGG2800.036.9642872
GGTACCT16450.036.930098
Produced by FastQC (version 0.11.5)