##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546558_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1902352
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.47923360135243	31.0	31.0	33.0	30.0	34.0
2	31.84384645954061	31.0	31.0	34.0	30.0	34.0
3	31.921080851493308	33.0	31.0	34.0	30.0	34.0
4	35.690533087462256	37.0	35.0	37.0	33.0	37.0
5	35.63926865269939	37.0	35.0	37.0	33.0	37.0
6	35.68500256524555	37.0	35.0	37.0	33.0	37.0
7	36.02181089514454	37.0	35.0	37.0	35.0	37.0
8	34.70283470146429	37.0	35.0	37.0	33.0	37.0
9	36.98946251797774	39.0	37.0	39.0	33.0	39.0
10	36.95912428404417	39.0	37.0	39.0	32.0	39.0
11	36.88809431692978	39.0	37.0	39.0	33.0	39.0
12	36.52353770490424	39.0	35.0	39.0	32.0	39.0
13	36.39192115864992	39.0	35.0	39.0	32.0	39.0
14	37.486424173864776	40.0	35.0	41.0	32.0	41.0
15	37.66948808632682	40.0	35.0	41.0	33.0	41.0
16	37.72341501467657	40.0	35.0	41.0	33.0	41.0
17	37.61545970461828	40.0	35.0	41.0	33.0	41.0
18	37.5337750321707	39.0	36.0	41.0	33.0	41.0
19	37.42795970461828	39.0	35.0	41.0	33.0	41.0
20	37.26121769262471	39.0	35.0	41.0	32.0	41.0
21	37.14441596507902	39.0	35.0	41.0	32.0	41.0
22	37.04931316601765	39.0	35.0	41.0	32.0	41.0
23	36.988587811298856	39.0	35.0	41.0	32.0	41.0
24	36.85326269796547	38.0	35.0	41.0	32.0	41.0
25	36.73502275078429	38.0	35.0	41.0	31.0	41.0
26	36.634743202099294	38.0	35.0	41.0	31.0	41.0
27	36.51498092887121	38.0	35.0	40.0	31.0	41.0
28	36.53305644801803	38.0	35.0	40.0	31.0	41.0
29	36.478521325180616	38.0	35.0	40.0	31.0	41.0
30	36.40382904951345	38.0	35.0	40.0	31.0	41.0
31	36.269938476160036	38.0	35.0	40.0	30.0	41.0
32	36.13401147631984	38.0	35.0	41.0	30.0	41.0
33	35.95254032902428	38.0	35.0	41.0	30.0	41.0
34	35.75056666694702	38.0	35.0	41.0	29.0	41.0
35	35.58989450953346	38.0	35.0	41.0	28.0	41.0
36	35.43606283169466	38.0	35.0	40.0	27.0	41.0
37	35.39196163486042	38.0	35.0	40.0	27.0	41.0
38	35.27173572503932	38.0	34.0	40.0	27.0	41.0
39	35.19529245901915	38.0	34.0	40.0	26.0	41.0
40	35.040846278711825	38.0	34.0	40.0	25.0	41.0
41	34.95428448573135	37.0	34.0	40.0	25.0	41.0
42	34.855490992203336	37.0	34.0	40.0	25.0	41.0
43	34.75435460945188	37.0	34.0	40.0	24.0	41.0
44	34.623530240460234	37.0	34.0	40.0	24.0	41.0
45	34.49706731456639	36.0	34.0	40.0	23.0	41.0
46	34.44329072642707	36.0	34.0	40.0	23.0	41.0
47	34.369364870434076	36.0	34.0	40.0	23.0	41.0
48	34.2841193427925	36.0	34.0	40.0	23.0	41.0
49	34.222698533184186	36.0	34.0	40.0	24.0	41.0
50	34.10859189045981	35.0	33.0	40.0	24.0	41.0
51	33.74180698419641	35.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	3.0
11	6.0
12	9.0
13	13.0
14	15.0
15	56.0
16	138.0
17	359.0
18	825.0
19	1561.0
20	2730.0
21	4498.0
22	7019.0
23	10261.0
24	15126.0
25	22421.0
26	30438.0
27	34818.0
28	36381.0
29	38821.0
30	44811.0
31	53375.0
32	66933.0
33	87251.0
34	145010.0
35	222662.0
36	153029.0
37	195962.0
38	277976.0
39	449478.0
40	366.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.040913563840974	23.40539500576129	26.589243210509938	15.964448219887803
2	34.20323893790423	24.579573075855574	27.848946987728873	13.368240998511316
3	29.398660184865893	24.347281680782526	32.371348730413715	13.882709403937863
4	25.19239341615011	27.338473636845332	31.809991000613973	15.659141946390573
5	23.212265658511146	30.916150113123127	30.827102450019765	15.044481778345965
6	20.60712213092004	39.91564126933396	28.57383912125621	10.903397478489785
7	84.03392221839071	4.243168456731457	9.520057276466185	2.2028520484116503
8	81.33268711573884	6.398027284119869	9.094216002085838	3.17506959805546
9	78.53793619687629	5.424127606247424	11.882396107555278	4.155540089321009
10	42.996932218643025	23.83812249257761	18.279424628039393	14.885520660739967
11	35.67373440877398	23.865036544235767	24.449996635743542	16.01123241124671
12	31.49916524386654	21.43467665290125	28.778375400556783	18.287782702675425
13	23.54506421524513	28.29870602286012	30.39952648090364	17.75670328099111
14	19.599685021489186	31.36606684777581	31.280856539694017	17.753391591040984
15	18.841518288939167	22.516022271377746	40.87844941419884	17.764010025484243
16	21.108448909560376	20.257081759842553	38.57587870173344	20.058590628863637
17	21.266411263530618	21.083637518188013	30.466601344020454	27.183349874260916
18	23.619182990319352	22.728285827228607	32.62929258097345	21.023238601478592
19	27.387413054997182	24.21686417655618	27.34309948947408	21.052623278972558
20	28.8790928282463	23.8927390935011	26.303123712120573	20.92504436613203
21	25.36428589451374	25.799378874151575	28.84371556893782	19.992619662396866
22	24.213762752634633	23.18361691211721	27.976578467076546	24.626041868171612
23	22.583570233058868	26.691537633413798	27.09640487144335	23.62848726208399
24	22.897444847220704	23.80469019403349	32.78609847178651	20.511766486959303
25	22.457883714475553	24.365049160197483	30.647745527641572	22.529321597685392
26	20.389759623876127	27.2609380388067	28.71293009916146	23.636372238155715
27	21.335799052961807	26.072882410826175	29.52986618669941	23.061452349512603
28	20.227591949334297	26.308012397285047	32.754611134006744	20.709784519373912
29	21.608093559972076	24.318264968838573	32.18605179272816	21.88758967846119
30	22.889612437656123	25.06502476933817	31.374477488918977	20.67088530408673
31	26.567533243059117	24.65784460499424	27.284698100036163	21.489924051910478
32	27.9200169053887	24.17039538424014	27.792490559055317	20.117097151315843
33	25.492337905918568	24.53068622421087	27.770202360025902	22.206773509844655
34	21.91587045930511	26.4266024374038	29.647142064139548	22.010385039151533
35	22.416671572874	24.99274582201401	30.264588257062837	22.325994348049154
36	26.371617870930304	26.782793089817236	26.691642766428085	20.153946272824378
37	23.26940545177759	27.533127412802678	28.09558903925246	21.10187809616727
38	23.465899055485	29.61260586894539	24.794780356106546	22.126714719463063
39	23.03427546531872	27.45522384921403	25.785133350715324	23.72536733475193
40	23.918128716452056	25.294057040968234	27.416219500912554	23.371594741667156
41	21.70933665273304	24.157726856018233	27.684518953379815	26.448417537868913
42	24.052646408235702	25.8897932664407	26.109784098841853	23.947776226481746
43	23.201594657560744	25.11401675399716	28.011219795284998	23.6731687931571
44	23.415330075611664	26.08386881081945	27.723733567709868	22.77706754585902
45	21.247697586987055	27.877911133165682	27.449441533428093	23.42494974641917
46	23.35219770053071	26.816172821854213	27.492756335315445	22.338873142299636
47	23.029965011732845	26.200829289216713	28.070882780894387	22.698322918156048
48	23.242964498683737	25.17814789271386	28.5958644877499	22.983023120852504
49	23.088629233706484	24.09674970773022	28.971977846371228	23.842643212192066
50	22.351068571957242	26.127078479692507	28.308535959696208	23.213316988654046
51	21.652722524538046	27.52750279653818	26.902749859121762	23.917024819802013
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2498.0
1	3078.5
2	3659.0
3	16307.5
4	28956.0
5	19805.0
6	10654.0
7	10766.0
8	10878.0
9	11526.0
10	12174.0
11	12524.0
12	12874.0
13	12744.0
14	12614.0
15	12105.5
16	11597.0
17	11058.0
18	10519.0
19	10185.0
20	9851.0
21	9687.5
22	9524.0
23	9687.5
24	9851.0
25	10268.0
26	12051.5
27	13418.0
28	15577.5
29	17737.0
30	20179.0
31	22621.0
32	25153.0
33	27685.0
34	31988.0
35	36291.0
36	40202.0
37	44113.0
38	52240.0
39	60367.0
40	74602.5
41	88838.0
42	105900.0
43	122962.0
44	135067.0
45	147172.0
46	151272.5
47	155373.0
48	149598.0
49	143823.0
50	136234.0
51	128645.0
52	121253.0
53	113861.0
54	107662.5
55	101464.0
56	99338.0
57	97212.0
58	93539.5
59	89867.0
60	84530.0
61	79193.0
62	73835.5
63	68478.0
64	63127.5
65	57777.0
66	48826.0
67	39875.0
68	34951.5
69	30028.0
70	26223.5
71	22419.0
72	18708.0
73	14997.0
74	12598.0
75	7988.0
76	5777.0
77	4338.0
78	2899.0
79	2302.5
80	1706.0
81	1180.5
82	655.0
83	504.5
84	354.0
85	230.0
86	106.0
87	81.5
88	57.0
89	37.0
90	17.0
91	14.5
92	12.0
93	11.0
94	10.0
95	8.5
96	7.0
97	5.0
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1902352.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.95247051249658
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.16487725607332	19.75359265357581
2	6.422077418333445	3.2049339481987094
3	2.383461139282817	1.7841973138690799
4	1.3184402280038185	1.315933636470182
5	0.8968383135898274	1.1189165787163664
6	0.6945333890978497	1.0398194346845044
7	0.5467085005404013	0.9549209417065909
8	0.48353757270509623	0.9652365619686467
9	0.4152093661889823	0.9324449519708677
>10	6.042744840931342	38.379429739470574
>50	1.5256521977995416	24.989482388705202
>100	0.10156094011574948	3.765619698230459
>500	0.003705011737208873	0.6068473997152962
>1k	4.358837337892792E-4	0.22546727477548328
>5k	0.0	0.0
>10k+	2.179418668946396E-4	0.9631574779422539
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17711	0.9310054080422551	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2283	0.12000933581166892	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.051330142896793E-4	0.0	0.0	0.05119977795907382	0.0
2	1.051330142896793E-4	0.0	0.0	0.16211510803468548	0.0
3	1.051330142896793E-4	0.0	0.0	0.2737138027031801	0.0
4	1.051330142896793E-4	0.0	0.0	0.44776150785974417	0.0
5	1.051330142896793E-4	0.0	0.0	0.7708878272790735	0.0
6	1.051330142896793E-4	0.0	0.0	1.3954830651740582	0.0
7	1.051330142896793E-4	0.0	0.0	1.7823199912529333	0.0
8	1.051330142896793E-4	0.0	0.0	2.737032894017511	0.0
9	1.051330142896793E-4	0.0	0.0	3.2939224707099424	0.0
10	1.051330142896793E-4	0.0	0.0	4.058397184117346	0.0
11	1.051330142896793E-4	0.0	0.0	4.501427706334054	0.0
12	1.051330142896793E-4	0.0	0.0	4.909974599863747	0.0
13	1.051330142896793E-4	0.0	0.0	5.114826278207188	0.0
14	1.051330142896793E-4	0.0	0.0	5.196672329831703	0.0
15	1.051330142896793E-4	0.0	0.0	5.282198036956357	0.0
16	1.051330142896793E-4	0.0	0.0	5.4378474646122275	0.0
17	1.051330142896793E-4	0.0	0.0	5.608425780297232	0.0
18	1.051330142896793E-4	0.0	0.0	5.8834537456790335	0.0
19	1.051330142896793E-4	0.0	0.0	6.008614599190897	0.0
20	1.051330142896793E-4	0.0	0.0	6.142922024945961	0.0
21	1.051330142896793E-4	0.0	0.0	6.297362422937501	0.0
22	1.051330142896793E-4	0.0	0.0	6.464891881208104	0.0
23	1.051330142896793E-4	0.0	0.0	6.650346518415099	0.0
24	1.051330142896793E-4	0.0	0.0	6.799582832199299	0.0
25	1.051330142896793E-4	0.0	0.0	6.92222049336821	0.0
26	1.051330142896793E-4	0.0	0.0	7.046750548794335	0.0
27	1.051330142896793E-4	0.0	0.0	7.173488397520543	0.0
28	1.051330142896793E-4	0.0	0.0	7.301067310361069	0.0
29	1.051330142896793E-4	0.0	0.0	7.439948022237735	0.0
30	1.051330142896793E-4	0.0	0.0	7.648794755124183	0.0
31	1.051330142896793E-4	0.0	0.0	7.802026123451391	0.0
32	1.051330142896793E-4	0.0	0.0	7.957044753021523	0.0
33	1.051330142896793E-4	0.0	0.0	8.121367654356291	0.0
34	1.051330142896793E-4	0.0	0.0	8.281432668612329	0.0
35	1.051330142896793E-4	0.0	0.0	8.457109935490383	0.0
36	1.051330142896793E-4	0.0	0.0	8.62053920620369	0.0
37	1.051330142896793E-4	0.0	0.0	8.77413854008091	0.0
38	1.051330142896793E-4	0.0	0.0	8.949605540930385	0.0
39	1.5769952143451896E-4	0.0	0.0	9.181266137917694	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTCGA	90	0.0	42.500004	14
CGTTTTT	10125	0.0	41.333332	1
AACCCGA	105	0.0	38.57143	24
GTACGAT	35	6.250446E-6	38.57143	22
ATTGGGC	580	0.0	38.40517	4
TCACGAC	300	0.0	38.250004	25
CGGTCTA	300	0.0	38.250004	31
CGCATCG	165	0.0	38.181816	21
TATAGCG	165	0.0	38.181816	1
TAGCATA	1510	0.0	38.145695	30
CGACGGT	290	0.0	38.01724	28
CGCCAGT	160	0.0	37.968754	26
GGGCGAT	4235	0.0	37.561985	7
ATAATCG	30	1.1402031E-4	37.500004	1
GCGTAAG	145	0.0	37.24138	1
AGGGTAC	940	0.0	37.101063	6
CATATGC	1555	0.0	37.041798	33
CAGTAGC	1630	0.0	36.993866	27
CGTAAGG	280	0.0	36.964287	2
GGTACCT	1645	0.0	36.93009	8
>>END_MODULE