FastQCFastQC Report
Thu 26 May 2016
SRR1546557_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546557_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2085787
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT191930.9201802485105142No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC29400.1409539900287038No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGGT9100.041.2912064
CGTTTTT125150.041.0447461
AGGGTAC12100.037.9338876
TAGGGTA9450.037.857145
TATTCCG301.1402408E-437.5000041
GTTGATC16500.037.0909116
CATATGC15800.036.8829133
CACGACG2750.036.81818426
TTGATCC16600.036.7319317
GCGTTAG1550.036.290321
GGACTAA12250.036.1836748
GGGTACC17800.036.1516887
TAGCATA16150.036.0835930
TAGTAGG9250.035.7567562
GCGATCG1450.035.6896559
GGGCGAT45350.035.6725467
CGGGTAT1900.035.5263186
AGGGCGA23950.035.5114866
GGCGATA10650.035.4929548
TGATCCT17200.035.4505818
Produced by FastQC (version 0.11.5)