FastQCFastQC Report
Thu 26 May 2016
SRR1546556_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546556_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1934174
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT362961.8765633288421828No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT222150.042.640111
CGTTCGA2150.039.7674414
CCAACGG700.038.5714262
AGGGTAC10050.038.283586
AGGGCGA25650.038.2456176
CGTTCGG4100.037.86585245
CGCGTAT301.1402101E-437.50000431
GGGCGAT45050.037.2086567
GTTGATC17700.036.99152416
TCACGAC1950.036.92307725
GTATGCG552.752131E-936.818181
TAATGCG552.752131E-936.818181
GTTTTTT256650.036.803042
CATATGC17250.036.2608733
TAGCATA17250.036.2608730
ACGGGCT5300.036.0849085
GGTACCT18100.036.0497258
CGTATAA250.002107463336.00000433
ACGGGTA1750.036.0000045
CGCAGTA1000.036.00000437
Produced by FastQC (version 0.11.5)