##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546556_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1934174
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.50089650672587	31.0	31.0	33.0	30.0	34.0
2	31.860668171529554	31.0	31.0	34.0	30.0	34.0
3	31.843306238218485	31.0	31.0	34.0	30.0	34.0
4	35.70102483023761	37.0	35.0	37.0	33.0	37.0
5	35.666672698526604	37.0	35.0	37.0	33.0	37.0
6	35.71905164685287	37.0	35.0	37.0	33.0	37.0
7	36.01140745351763	37.0	35.0	37.0	35.0	37.0
8	34.675170382809405	37.0	35.0	37.0	33.0	37.0
9	36.98184806537571	39.0	37.0	39.0	33.0	39.0
10	36.99886101250456	39.0	37.0	39.0	32.0	39.0
11	36.960756891572316	39.0	37.0	39.0	33.0	39.0
12	36.7355134543221	39.0	35.0	39.0	33.0	39.0
13	36.68736576957399	39.0	35.0	39.0	33.0	39.0
14	37.816937876323436	40.0	36.0	41.0	33.0	41.0
15	37.941669673979696	40.0	36.0	41.0	33.0	41.0
16	37.9493463359553	40.0	36.0	41.0	33.0	41.0
17	37.836379767280505	40.0	36.0	41.0	33.0	41.0
18	37.663331220458964	39.0	36.0	41.0	33.0	41.0
19	37.48127107488778	39.0	36.0	41.0	33.0	41.0
20	37.210576711298984	39.0	35.0	41.0	32.0	41.0
21	37.12798693395734	39.0	35.0	41.0	32.0	41.0
22	37.054896301987306	39.0	35.0	41.0	32.0	41.0
23	36.96700968992448	38.0	35.0	41.0	32.0	41.0
24	36.80580547561905	38.0	35.0	41.0	32.0	41.0
25	36.68880721176068	38.0	35.0	41.0	31.0	41.0
26	36.59753827732148	38.0	35.0	41.0	31.0	41.0
27	36.47692968678103	38.0	35.0	40.0	31.0	41.0
28	36.45340698406658	38.0	35.0	40.0	31.0	41.0
29	36.33706636528048	38.0	35.0	40.0	31.0	41.0
30	36.21366640229886	38.0	35.0	40.0	30.0	41.0
31	36.00328357221222	38.0	35.0	40.0	30.0	41.0
32	35.70218501541226	38.0	35.0	40.0	29.0	41.0
33	35.32504624713185	38.0	35.0	41.0	25.0	41.0
34	34.923050873396086	38.0	34.0	41.0	23.0	41.0
35	34.64217645361793	38.0	34.0	41.0	21.0	41.0
36	34.46431034643212	38.0	34.0	40.0	21.0	41.0
37	34.399356004165085	38.0	34.0	40.0	20.0	41.0
38	34.27114054888547	38.0	34.0	40.0	20.0	41.0
39	34.164490371600486	38.0	34.0	40.0	18.0	41.0
40	34.006513374701555	37.0	33.0	40.0	18.0	41.0
41	33.8792786998481	37.0	33.0	40.0	18.0	41.0
42	33.78382761840455	37.0	33.0	40.0	18.0	41.0
43	33.67014239670267	37.0	33.0	40.0	18.0	41.0
44	33.53650033554375	36.0	33.0	40.0	17.0	41.0
45	33.420139036094994	36.0	33.0	40.0	18.0	41.0
46	33.35700769424054	36.0	33.0	40.0	18.0	41.0
47	33.28713342232912	36.0	33.0	40.0	18.0	41.0
48	33.20366471682485	35.0	33.0	40.0	17.0	41.0
49	33.12598349476314	35.0	33.0	40.0	17.0	41.0
50	33.0346049528119	35.0	32.0	40.0	17.0	41.0
51	32.69128320409643	35.0	32.0	39.0	15.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	1.0
11	8.0
12	10.0
13	3.0
14	23.0
15	55.0
16	195.0
17	437.0
18	989.0
19	1936.0
20	3464.0
21	5680.0
22	8772.0
23	13129.0
24	19990.0
25	31737.0
26	45943.0
27	53090.0
28	51240.0
29	48789.0
30	49737.0
31	55923.0
32	66209.0
33	83481.0
34	131941.0
35	187613.0
36	154875.0
37	193227.0
38	274751.0
39	450534.0
40	390.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.1786075089418	21.12359074209456	24.167008759294664	21.530792989668974
2	39.935290206568794	22.508884929690918	24.79963022975182	12.756194633988462
3	28.205011544979925	23.147400389003263	35.494117902525836	13.15347016349098
4	24.586826211085455	25.60793392941897	35.15903946594256	14.646200393553011
5	21.660098832886803	28.766543237578418	34.703858081020634	14.869499848514145
6	19.493334105411407	37.90372531116642	32.799375857601234	9.803564725820944
7	78.83230774480477	3.882329097588945	15.207783787808129	2.077579369798167
8	76.33118840393884	6.257348097947754	14.598841676084984	2.8126218220284214
9	73.57631733235996	5.046391896489148	17.401640183354754	3.975650587796134
10	37.54858663181286	24.435030147235977	23.733490368498387	14.282892852452777
11	30.245469125321712	23.943812707646778	30.118903469904986	15.691814697126524
12	26.759588330729294	21.025254191194794	34.542755718978746	17.672401759097163
13	23.502694173326702	23.12832247770883	35.679158131584856	17.689825217379614
14	19.79020501774918	26.234816515990804	36.375941357912986	17.599037108347027
15	19.25018121430647	21.68382989327744	40.91364065487386	18.152348237542228
16	22.59729476251878	20.141776282795654	38.78658279968607	18.4743461549995
17	21.60710463484671	20.799111145119312	35.40829315252919	22.185491067504785
18	22.99322604894906	21.461254261509048	36.33054730339669	19.214972386145195
19	25.603073973696265	23.941279326472177	31.786592105984262	18.669054593847296
20	26.259167996260935	23.4705874445629	31.226508059771252	19.043736499404915
21	24.56991976936925	24.259658127965736	33.241735231680295	17.92868687098472
22	22.882119188863047	21.996986827451927	32.989637953979326	22.131256029705703
23	21.52081456994045	24.91482152071117	32.39548251605078	21.168881393297603
24	21.877297492366253	23.120877439154906	35.97964815988634	19.022176908592506
25	22.875966691724734	23.3683732694163	33.291575628666294	20.46408441019267
26	20.043594836865765	24.20904220613037	33.319701329870014	22.42766162713386
27	20.350961185498306	22.971873264763147	34.26403208811617	22.41313346162238
28	20.323042290921087	24.291713155072912	35.15914286925582	20.226101684750184
29	21.474903498857913	23.80204676518245	34.66141102093193	20.06163871502771
30	22.142009974283596	23.695696457505893	35.14275344410586	19.019540124104655
31	24.723473689543958	23.3856933243855	31.967185992573572	19.923646993496966
32	24.811573312432078	23.996341590777252	31.353073715187985	19.83901138160269
33	24.29543567434988	24.467291980969655	30.4084844486587	20.828787896021765
34	21.50282239343513	25.80052260034516	32.60006597131385	20.096589034905858
35	21.284486297509943	26.473884976222408	32.18996843096846	20.051660295299182
36	24.079891467882415	27.66447072497097	28.453851618313553	19.801786188833063
37	22.469384864029813	28.45286928683769	28.830291380196403	20.247454468936095
38	23.556567299529412	28.204804738353427	27.652010625724472	20.586617336392692
39	22.635657391734146	26.773651181331154	27.387660055403494	23.203031371531207
40	23.72072005931214	24.722904971321093	29.726591299438414	21.82978366992835
41	21.407277731993087	24.824808936527944	29.844005761632612	23.923907569846353
42	22.853993487659334	26.358900491889557	27.72222147542052	23.06488454503059
43	21.90257960245562	25.139413517087917	29.477285911195168	23.4807209692613
44	22.167240382716344	25.659997497639818	29.988253383614914	22.18450873602892
45	21.61196459056941	26.36432916583513	29.49207258498977	22.531633658605692
46	22.442396599271834	26.24153773135199	29.577793931673156	21.73827173770302
47	21.842967592367597	26.56477648856825	29.902842246871273	21.689413672192885
48	21.64112432490562	25.38613382249994	30.888844540356764	22.08389731223768
49	22.508626421407797	24.599182907018708	30.51380072320277	22.378389948370724
50	21.157662133810092	25.39052846331302	31.3613459802479	22.09046342262899
51	21.025926312730913	26.460752755439792	30.302030737668893	22.2112901941604
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2243.0
1	3321.0
2	4399.0
3	33625.5
4	62852.0
5	42704.5
6	22557.0
7	22419.5
8	22282.0
9	22259.5
10	22237.0
11	22284.0
12	22331.0
13	21378.5
14	20426.0
15	19387.5
16	18349.0
17	17468.5
18	16588.0
19	15691.0
20	14794.0
21	14406.5
22	14019.0
23	13760.0
24	13501.0
25	14055.5
26	15891.5
27	17173.0
28	18790.0
29	20407.0
30	23148.0
31	25889.0
32	29041.5
33	32194.0
34	36635.5
35	41077.0
36	45631.0
37	50185.0
38	55673.5
39	61162.0
40	70126.0
41	79090.0
42	88649.0
43	98208.0
44	108609.5
45	119011.0
46	125659.5
47	132308.0
48	131765.0
49	131222.0
50	125626.0
51	120030.0
52	114532.0
53	109034.0
54	104200.0
55	99366.0
56	97773.0
57	96180.0
58	92616.0
59	89052.0
60	84100.5
61	79149.0
62	73039.0
63	66929.0
64	62117.0
65	57305.0
66	49615.5
67	41926.0
68	36219.0
69	30512.0
70	26754.0
71	22996.0
72	20274.5
73	17553.0
74	14388.0
75	9033.5
76	6844.0
77	5136.5
78	3429.0
79	2656.0
80	1883.0
81	1378.5
82	874.0
83	681.0
84	488.0
85	327.5
86	167.0
87	115.5
88	64.0
89	48.5
90	33.0
91	22.5
92	12.0
93	8.5
94	5.0
95	3.0
96	1.0
97	1.5
98	2.0
99	2.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1934174.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.986394982946802
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.59999844318551	23.11585017487763
2	6.319901557507859	3.9166193182855835
3	2.9485443234942217	2.7409427709755287
4	1.8833321641061045	2.3343069728431898
5	1.4583982808236953	2.2595252586026797
6	1.1978027745436972	2.226935393020835
7	1.0372182734066615	2.2497758572316244
8	0.9152649434335047	2.2688608841020033
9	0.8361012157381603	2.3316986264926185
>10	8.660559975479913	49.454832530057686
>50	0.10659597711405393	2.1630226791173865
>100	0.03149024298168497	1.8737672065767768
>500	0.0035938711387179002	0.7621872585580057
>1k	0.0010268203253479716	0.37693084490574724
>5k	0.0	0.0
>10k+	1.7113672089132857E-4	1.9247442243527015
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	36296	1.8765633288421828	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0358809496973902	0.0
2	0.0	0.0	0.0	0.10903879382103161	0.0
3	0.0	0.0	0.0	0.225677731165862	0.0
4	0.0	0.0	0.0	0.33668118793862395	0.0
5	0.0	0.0	0.0	0.5647371953092121	0.0
6	0.0	0.0	0.0	1.1127230538720922	0.0
7	0.0	0.0	0.0	1.4662589818702971	0.0
8	0.0	0.0	0.0	2.2282379972019064	0.0
9	0.0	0.0	0.0	2.735793160284442	0.0
10	0.0	0.0	0.0	3.3942137573972144	0.0
11	0.0	0.0	0.0	3.7678616298223426	0.0
12	0.0	0.0	0.0	4.077864762942734	0.0
13	0.0	0.0	0.0	4.2567524948634405	0.0
14	0.0	0.0	0.0	4.364964062178481	0.0
15	0.0	0.0	0.0	4.442516547115202	0.0
16	0.0	0.0	0.0	4.549797484611002	0.0
17	0.0	0.0	0.0	4.658164156895915	0.0
18	0.0	0.0	0.0	4.839326761708099	0.0
19	0.0	0.0	0.0	4.920291555982037	0.0
20	0.0	0.0	0.0	5.012061996490491	0.0
21	0.0	0.0	0.0	5.115620414709328	0.0
22	0.0	0.0	0.0	5.22455580521711	0.0
23	0.0	0.0	0.0	5.351845283826584	0.0
24	0.0	0.0	0.0	5.464141282014959	0.0
25	0.0	0.0	0.0	5.555394705957168	0.0
26	0.0	0.0	0.0	5.645458991797015	0.0
27	0.0	0.0	0.0	5.752378017696443	0.0
28	0.0	0.0	0.0	5.857280678987516	0.0
29	0.0	0.0	0.0	5.965233738019434	0.0
30	0.0	0.0	0.0	6.114237912411189	0.0
31	0.0	0.0	0.0	6.230463236503024	0.0
32	0.0	0.0	0.0	6.3671107149615285	0.0
33	0.0	0.0	0.0	6.499208447637079	0.0
34	0.0	0.0	0.0	6.632650423384866	0.0
35	0.0	0.0	0.0	6.778500796722528	0.0
36	0.0	0.0	0.0	6.911787667500442	0.0
37	0.0	0.0	0.0	7.0534502066515214	0.0
38	0.0	0.0	0.0	7.235336634656448	0.0
39	0.0	0.0	0.0	7.485986265971934	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	22215	0.0	42.64011	1
CGTTCGA	215	0.0	39.76744	14
CCAACGG	70	0.0	38.571426	2
AGGGTAC	1005	0.0	38.28358	6
AGGGCGA	2565	0.0	38.245617	6
CGTTCGG	410	0.0	37.865852	45
CGCGTAT	30	1.1402101E-4	37.500004	31
GGGCGAT	4505	0.0	37.208656	7
GTTGATC	1770	0.0	36.991524	16
TCACGAC	195	0.0	36.923077	25
GTATGCG	55	2.752131E-9	36.81818	1
TAATGCG	55	2.752131E-9	36.81818	1
GTTTTTT	25665	0.0	36.80304	2
CATATGC	1725	0.0	36.26087	33
TAGCATA	1725	0.0	36.26087	30
ACGGGCT	530	0.0	36.084908	5
GGTACCT	1810	0.0	36.049725	8
CGTATAA	25	0.0021074633	36.000004	33
ACGGGTA	175	0.0	36.000004	5
CGCAGTA	100	0.0	36.000004	37
>>END_MODULE