FastQCFastQC Report
Thu 26 May 2016
SRR1546555_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546555_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1546687
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT189411.2246175211920705No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT135750.042.8979761
CCGATGA6950.039.56834418
CGATGAA7300.037.3698619
GTTTTTT157750.037.2361342
ACGGGAT3900.037.230775
CGGGGTA2450.036.8163266
CGACCCG8350.036.62275332
GCAACGA1700.036.23529411
GCGCGAC8400.036.1428579
TCAGACG8650.035.60693722
TAAGGGA12550.035.5856554
TAGGGCG5750.035.582615
ACCCGCT8600.035.5581434
TACGGGA3050.035.344264
TAGGGAC10550.035.2417075
GCGAACC250.002352995635.233
ACGGGAC2500.035.25
TAGGACG751.8189894E-1235.21
CGGTAGT2250.035.212
CTTACCG250.002352995635.21
Produced by FastQC (version 0.11.5)