##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546555_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1546687
Sequences flagged as poor quality	0
Sequence length	50
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.58874549278555	31.0	30.0	31.0	27.0	34.0
2	30.930890348208784	31.0	31.0	33.0	28.0	34.0
3	31.02781041025107	31.0	31.0	34.0	28.0	34.0
4	34.88859801627608	35.0	35.0	37.0	32.0	37.0
5	34.70206706334248	35.0	35.0	37.0	32.0	37.0
6	34.73482417580286	35.0	35.0	37.0	32.0	37.0
7	35.35289622270052	35.0	35.0	37.0	33.0	37.0
8	34.311375216834435	35.0	35.0	37.0	32.0	37.0
9	36.42674051052346	39.0	37.0	39.0	32.0	39.0
10	35.91857369978541	38.0	35.0	39.0	30.0	39.0
11	35.59226979990134	37.0	35.0	39.0	30.0	39.0
12	35.10686648300529	37.0	34.0	39.0	30.0	39.0
13	34.900703245065095	37.0	33.0	39.0	27.0	39.0
14	35.783731291463624	38.0	34.0	40.0	27.0	41.0
15	35.989443242233236	38.0	34.0	40.0	29.0	41.0
16	36.07704273715367	38.0	34.0	40.0	30.0	41.0
17	35.92613566933711	38.0	34.0	40.0	29.0	41.0
18	35.79008487172906	38.0	34.0	40.0	29.0	41.0
19	35.707766341864904	37.0	34.0	40.0	29.0	41.0
20	35.47083217224946	37.0	34.0	40.0	29.0	41.0
21	35.29393859261764	37.0	33.0	40.0	28.0	41.0
22	35.25422596814999	36.0	33.0	40.0	28.0	41.0
23	35.162233858563496	36.0	33.0	40.0	28.0	41.0
24	35.085308792276656	36.0	33.0	40.0	27.0	41.0
25	34.90248059238876	36.0	33.0	40.0	27.0	41.0
26	34.67581676189171	36.0	33.0	40.0	26.0	41.0
27	34.48077083469377	36.0	33.0	40.0	25.0	41.0
28	34.44485406549612	36.0	33.0	39.0	25.0	41.0
29	34.22514251429022	36.0	33.0	39.0	24.0	41.0
30	34.14421793161771	36.0	33.0	39.0	24.0	41.0
31	34.018840269556804	36.0	33.0	39.0	23.0	41.0
32	33.65308947447027	35.0	32.0	39.0	23.0	41.0
33	33.36497300358767	35.0	32.0	39.0	21.0	41.0
34	33.06473966613801	35.0	31.0	39.0	19.0	41.0
35	32.799975043431544	35.0	31.0	39.0	18.0	41.0
36	32.746094070746054	35.0	31.0	39.0	18.0	41.0
37	32.76328306890793	35.0	31.0	39.0	18.0	41.0
38	32.735982134717624	35.0	31.0	39.0	18.0	41.0
39	32.620442274358034	35.0	31.0	39.0	18.0	41.0
40	32.52017699767309	35.0	31.0	39.0	17.0	41.0
41	32.53404082403227	35.0	31.0	39.0	18.0	41.0
42	32.35177705637921	35.0	30.0	39.0	16.0	41.0
43	32.38021655318755	35.0	30.0	39.0	17.0	41.0
44	32.0828247732088	35.0	30.0	39.0	15.0	41.0
45	32.00286871228633	35.0	30.0	39.0	15.0	40.0
46	31.930135185722772	35.0	30.0	39.0	15.0	40.0
47	31.857336358293566	35.0	30.0	39.0	15.0	40.0
48	31.866267706394378	35.0	30.0	39.0	15.0	40.0
49	31.86697373159534	35.0	30.0	39.0	15.0	40.0
50	31.36457667259116	35.0	29.0	38.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	3.0
12	6.0
13	7.0
14	39.0
15	115.0
16	325.0
17	926.0
18	1860.0
19	3548.0
20	5966.0
21	9139.0
22	13343.0
23	18754.0
24	25006.0
25	33490.0
26	42851.0
27	50013.0
28	53797.0
29	57123.0
30	63952.0
31	74298.0
32	86772.0
33	104636.0
34	142070.0
35	148297.0
36	132098.0
37	151934.0
38	172003.0
39	154313.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.39032848921598	22.625715480895618	26.006619309530628	18.97733672035777
2	37.71913774409431	23.555121365861353	25.979270531141722	12.74647035890261
3	28.38040275763616	24.603232586812975	33.96065267245409	13.055711983096774
4	26.073989113505192	25.90103880099852	33.61559255363238	14.409379531863914
5	22.934957105089783	29.771828430703824	32.721229311425	14.571985152781398
6	20.44873979027431	39.269677704668105	30.455806507716172	9.825775997341415
7	82.75242502199863	3.3862701373969	12.22470997687315	1.6365948637313172
8	81.08214525628003	5.934232330135315	11.307265141557405	1.6763572720272426
9	79.35102577315256	4.348003183578837	13.171055294316176	3.129915748952438
10	44.88406510173034	26.163535350074064	17.88674760956806	11.065651938627532
11	34.7560948013399	23.275556075663662	27.293951523482125	14.674397599514316
12	31.277692254476825	20.936427344381894	31.018816347457502	16.76706405368378
13	24.061106093217308	25.24221125541237	31.62217048439665	19.07451216697367
14	18.90266097794835	28.36585553508887	34.29536809968662	18.436115387276157
15	19.63674615484581	22.390373747241686	39.821825618240794	18.15105447967171
16	23.837854717858235	20.475571334083757	37.37918531674475	18.307388631313252
17	22.774937657069593	21.05105945805454	32.19953358371797	23.974469301157892
18	24.404873125590377	22.636965332998855	33.497598415193245	19.460563126217522
19	26.90460319379422	24.894047729113904	29.51683178303044	18.684517294061436
20	29.434720793541292	24.061429364829472	28.770397630548388	17.733452211080845
21	26.02633887787251	25.408825444320666	30.800349391958427	17.764486285848395
22	24.0339512777957	21.44945939288298	32.617071197986405	21.89951813133491
23	23.725420851148293	24.500561522790328	31.454457172007004	20.319560454054375
24	23.780635642505562	22.849548745156582	34.089379428417	19.280436183920855
25	22.96456878476382	23.296310113164463	32.43739683594677	21.30172426612495
26	20.704382981172014	26.426419825084196	31.748957610686585	21.120239583057206
27	20.94231088772324	25.09169599278975	32.71715608911176	21.24883703037525
28	20.12941209178069	25.569685398532478	34.57693767387972	19.723964835807113
29	22.332184857052525	24.675451464969964	33.79209885387283	19.200264824104682
30	23.07506302180079	24.195716392521565	33.30428199112038	19.42493859455727
31	27.86724140049021	22.541535553088636	29.485215819360995	20.10600722706016
32	28.366308115345895	23.8563458540739	28.888327114665085	18.889018915915116
33	27.79592768284727	24.34299893902257	28.07051459021767	19.790558787912488
34	22.8553676341755	25.55850020075167	30.261132342872216	21.324999822200613
35	22.32804698041685	26.39208837987259	30.150314834223085	21.12954980548747
36	25.691494141995115	28.287817767912966	26.59458571773087	19.426102372361054
37	24.205479195208856	29.032635562334203	25.946167518056335	20.815717724400606
38	23.443075425086008	30.39761761752701	24.849112975023388	21.310193982363593
39	23.95436180688142	27.581857221273598	26.061187557663573	22.402593414181407
40	24.08864883457351	24.904069149090926	27.78862174441241	23.21866027192315
41	21.12948515116504	25.518673138133313	27.98885618098555	25.3629855297161
42	22.316538511023882	26.38258419447503	26.033386199017645	25.26749109548344
43	21.712149904925816	25.289926145367485	27.134707927331124	25.863216022375568
44	21.93688832970084	25.77418702038615	29.095932143995523	23.192992505917488
45	22.345826919085763	27.96545131626502	27.18759516308083	22.501126601568387
46	23.043899638388375	27.94346884663801	27.88431014161236	21.128321373361256
47	21.85510061182385	26.60312008829194	28.594214601920104	22.9475646979641
48	22.33360725214604	24.827971011587994	30.714941032025227	22.12348070424074
49	23.248207297274757	24.35754616156986	30.571861016482327	21.82238552467306
50	20.922203393446768	26.737924350563496	29.672519391447654	22.66735286454208
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	544.0
1	1490.5
2	2437.0
3	18528.5
4	34620.0
5	25059.0
6	15498.0
7	15048.5
8	14599.0
9	14786.0
10	14973.0
11	14786.5
12	14600.0
13	13795.5
14	12991.0
15	12265.0
16	11539.0
17	10655.5
18	9772.0
19	9435.5
20	9099.0
21	8777.0
22	8455.0
23	8563.0
24	8671.0
25	9769.5
26	10868.0
27	12997.5
28	15127.0
29	16498.5
30	17870.0
31	21040.5
32	24211.0
33	28100.5
34	31990.0
35	35102.0
36	38214.0
37	43052.5
38	47891.0
39	52759.0
40	57627.0
41	68595.5
42	79564.0
43	86975.5
44	94387.0
45	93979.5
46	93572.0
47	94980.5
48	96389.0
49	98548.5
50	100708.0
51	96332.0
52	91956.0
53	88199.0
54	84442.0
55	83591.0
56	82740.0
57	84425.0
58	86110.0
59	85560.0
60	85010.0
61	80788.5
62	76567.0
63	67834.0
64	59101.0
65	49704.5
66	40308.0
67	33500.0
68	26692.0
69	22572.0
70	18452.0
71	15666.5
72	12881.0
73	10415.5
74	7950.0
75	5999.0
76	4048.0
77	3232.5
78	2417.0
79	1735.5
80	1054.0
81	730.0
82	406.0
83	297.5
84	189.0
85	141.0
86	93.0
87	58.0
88	23.0
89	22.0
90	21.0
91	13.0
92	5.0
93	4.5
94	4.0
95	2.5
96	1.0
97	0.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1546687.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.05953372804882
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.49326727672691	22.692924139453766
2	5.759577106884599	3.462604046073909
3	2.717626021864936	2.450717131934166
4	1.8905723913542665	2.2731889825292595
5	1.4451432522206633	2.1720166165994597
6	1.2163796365356627	2.1938282826333313
7	1.0831353327713922	2.2790980147238136
8	0.8965679254043001	2.1560331034541855
9	0.8247775711347284	2.23131862958963
>10	8.427617167584673	50.63360970016723
>50	0.2100650112538615	3.9599570256525203
>100	0.03329961961586103	1.8450788476582096
>500	0.0015335340596538578	0.329661682655885
>1k	2.1907629423626536E-4	0.07263620374938896
>5k	0.0	0.0
>10k+	2.1907629423626536E-4	1.2473275931252732
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18941	1.2246175211920705	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0047844198599975305	0.0
2	0.0	0.0	0.0	0.023404864720528457	0.0
3	0.0	0.0	0.0	0.04357701331943697	0.0
4	0.0	0.0	0.0	0.07234818680185455	0.0
5	0.0	0.0	0.0	0.14547222547289787	0.0
6	0.0	0.0	0.0	0.27840151239391037	0.0
7	0.0	0.0	0.0	0.38708542840277316	0.0
8	0.0	0.0	0.0	0.6301856807485936	0.0
9	0.0	0.0	0.0	0.8066273266666106	0.0
10	0.0	0.0	0.0	1.0064091829827237	0.0
11	0.0	0.0	0.0	1.1131534693186145	0.0
12	0.0	0.0	0.0	1.2080660146493765	0.0
13	0.0	0.0	0.0	1.2545524724782713	0.0
14	0.0	0.0	0.0	1.276858213717449	0.0
15	0.0	0.0	0.0	1.2981941401201407	0.0
16	0.0	0.0	0.0	1.3322023137195826	0.0
17	0.0	0.0	0.0	1.3665337589311866	0.0
18	0.0	0.0	0.0	1.4277614022746683	0.0
19	0.0	0.0	0.0	1.4643557487714063	0.0
20	0.0	0.0	0.0	1.5024371446840892	0.0
21	0.0	0.0	0.0	1.5445271085875811	0.0
22	0.0	0.0	0.0	1.5917247639632324	0.0
23	0.0	0.0	0.0	1.6389224193388836	0.0
24	0.0	0.0	0.0	1.6784262103450796	0.0
25	0.0	0.0	0.0	1.7142447049726286	0.0
26	0.0	0.0	0.0	1.7523261008853117	0.0
27	0.0	0.0	0.0	1.7924764351158315	0.0
28	0.0	0.0	0.0	1.8332086582482428	0.0
29	0.0	0.0	0.0	1.8804709679463265	0.0
30	0.0	0.0	0.0	1.947452845986292	0.0
31	0.0	0.0	0.0	2.005641736175451	0.0
32	0.0	0.0	0.0	2.068162465967581	3.232716121619953E-4
33	0.0	0.0	0.0	2.1337219489140336	3.232716121619953E-4
34	0.0	0.0	0.0	2.1926866909723817	3.232716121619953E-4
35	0.0	0.0	0.0	2.258892717143158	3.232716121619953E-4
36	0.0	0.0	0.0	2.327232335954204	3.232716121619953E-4
37	0.0	0.0	0.0	2.3984167449522755	3.232716121619953E-4
38	0.0	0.0	0.0	2.5040619078068156	3.232716121619953E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	13575	0.0	42.897976	1
CCGATGA	695	0.0	39.568344	18
CGATGAA	730	0.0	37.36986	19
GTTTTTT	15775	0.0	37.236134	2
ACGGGAT	390	0.0	37.23077	5
CGGGGTA	245	0.0	36.816326	6
CGACCCG	835	0.0	36.622753	32
GCAACGA	170	0.0	36.235294	11
GCGCGAC	840	0.0	36.142857	9
TCAGACG	865	0.0	35.606937	22
TAAGGGA	1255	0.0	35.585655	4
TAGGGCG	575	0.0	35.58261	5
ACCCGCT	860	0.0	35.55814	34
TACGGGA	305	0.0	35.34426	4
TAGGGAC	1055	0.0	35.241707	5
GCGAACC	25	0.0023529956	35.2	33
ACGGGAC	250	0.0	35.2	5
TAGGACG	75	1.8189894E-12	35.2	1
CGGTAGT	225	0.0	35.2	12
CTTACCG	25	0.0023529956	35.2	1
>>END_MODULE