FastQCFastQC Report
Thu 26 May 2016
SRR1546554_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546554_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1174011
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35370.3012748602866583No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTCTA650.044.00000431
ACACCGT207.8565517E-444.027
TACGATC207.8565517E-444.039
TATTACG254.442898E-544.01
CGTACTA551.8189894E-1244.032
TAAGCGA207.8565517E-444.030
TACGTTA207.8565517E-444.044
CGTTTTT27950.042.4257621
TATAGCG800.041.251
CACAACG800.041.2512
TACACGG557.8216544E-1140.02
ATTAACG357.2903404E-637.7142871
TAATGCG357.2903404E-637.7142871
TACGTAG1050.037.7142871
ACGTAGG1700.037.529412
CGGGATC1950.037.2307746
GCGATGT1300.037.230779
TTGTACG1250.036.961
ATTTACG601.9826984E-1036.6666641
GGGCGAT10700.036.1869167
Produced by FastQC (version 0.11.5)