FastQCFastQC Report
Thu 26 May 2016
SRR1546553_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546553_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences813925
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41560.5106121571397856No Hit
GTACAAGGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACT8570.10529225665755444No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT8370.10283502779740149No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTACG408.307325E-944.01
GCGTAGT302.5268018E-644.016
GTACGTC207.855126E-444.028
CGTTTTT32500.042.172311
CGGGTAT750.041.0666666
CGACGGT1300.040.61538328
CGGTCTA1300.040.61538331
TATAGCG603.6379788E-1240.3333321
CGACCCG5850.040.23931532
ACCCGCT5950.039.56302634
CCGCTGA6050.039.27272836
GCGCGAC6300.039.111119
TTACGGG1800.039.111113
CGCTGAA6200.039.03225737
CACGACG1300.038.92307726
TCAGACG6350.038.80314622
AGACGTG6200.038.6774224
ATAGGGC3700.038.648654
ACGTAAG404.1225576E-738.51
AGGGCGC3600.038.56
Produced by FastQC (version 0.11.5)