##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546553_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	813925
Sequences flagged as poor quality	0
Sequence length	50
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.595769880517246	31.0	30.0	31.0	28.0	33.0
2	30.916884233805327	31.0	31.0	33.0	28.0	34.0
3	31.071382498387443	31.0	31.0	34.0	28.0	34.0
4	34.90542740424486	35.0	35.0	37.0	32.0	37.0
5	34.694178210523084	35.0	35.0	37.0	32.0	37.0
6	34.72540590349234	35.0	35.0	37.0	32.0	37.0
7	35.38325029947477	35.0	35.0	37.0	33.0	37.0
8	34.363497865282426	35.0	35.0	37.0	32.0	37.0
9	36.506730964155174	39.0	37.0	39.0	32.0	39.0
10	35.91831556961637	38.0	35.0	39.0	30.0	39.0
11	35.58865866019596	37.0	35.0	39.0	30.0	39.0
12	34.80435912399791	37.0	33.0	39.0	29.0	39.0
13	34.35479436065977	37.0	33.0	39.0	25.0	39.0
14	35.20337254661056	37.0	33.0	40.0	25.0	41.0
15	35.62168995914857	37.0	33.0	40.0	28.0	41.0
16	35.8228473139417	37.0	34.0	40.0	30.0	41.0
17	35.76040667137636	37.0	34.0	40.0	29.0	41.0
18	35.69613416469576	37.0	34.0	40.0	29.0	41.0
19	35.64336271769512	37.0	34.0	40.0	29.0	41.0
20	35.43833768467611	36.0	34.0	40.0	29.0	41.0
21	35.22635009368185	36.0	33.0	40.0	28.0	41.0
22	35.195490985041616	36.0	33.0	40.0	27.0	41.0
23	35.16722671007771	36.0	33.0	40.0	28.0	41.0
24	35.09522130417422	36.0	33.0	40.0	27.0	41.0
25	34.886482169733085	36.0	33.0	40.0	27.0	41.0
26	34.71546764136745	36.0	33.0	40.0	27.0	41.0
27	34.50696194366803	35.0	33.0	39.0	25.0	41.0
28	34.537458611051385	36.0	33.0	39.0	25.0	41.0
29	34.389426544214764	36.0	33.0	39.0	25.0	41.0
30	34.410687716927235	36.0	33.0	39.0	25.0	41.0
31	34.295690634886505	36.0	33.0	39.0	25.0	41.0
32	34.04627084805111	35.0	33.0	39.0	24.0	41.0
33	33.888559756734345	35.0	32.0	39.0	23.0	41.0
34	33.78711306324293	35.0	32.0	39.0	23.0	41.0
35	33.58839573670793	35.0	32.0	39.0	23.0	41.0
36	33.46226863654513	35.0	32.0	39.0	22.0	41.0
37	33.4540565776945	35.0	32.0	39.0	23.0	41.0
38	33.498354270970914	35.0	32.0	39.0	23.0	41.0
39	33.431950118254136	35.0	32.0	39.0	23.0	41.0
40	33.290707374758114	35.0	31.0	39.0	22.0	41.0
41	33.336580151733884	35.0	32.0	39.0	22.0	41.0
42	33.19760911631907	35.0	31.0	39.0	22.0	41.0
43	33.21824492428664	35.0	32.0	39.0	22.0	41.0
44	32.90872254814633	35.0	31.0	39.0	21.0	41.0
45	32.83046472340818	35.0	31.0	39.0	21.0	40.0
46	32.80039438523205	35.0	31.0	39.0	21.0	40.0
47	32.72680652394262	35.0	31.0	39.0	21.0	40.0
48	32.75424148416623	35.0	31.0	39.0	21.0	40.0
49	32.777470897195684	35.0	31.0	39.0	21.0	40.0
50	32.209079460638264	35.0	30.0	38.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	2.0
12	4.0
13	7.0
14	17.0
15	42.0
16	139.0
17	331.0
18	684.0
19	1411.0
20	2429.0
21	3719.0
22	5447.0
23	7765.0
24	10574.0
25	14154.0
26	18265.0
27	22323.0
28	24895.0
29	28850.0
30	33526.0
31	40364.0
32	48383.0
33	59407.0
34	83974.0
35	90711.0
36	67628.0
37	77482.0
38	88198.0
39	83193.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.41702245292871	23.100654237184017	27.90699388764321	13.575329422244064
2	33.20490217157601	24.582731824185274	28.911386184230736	13.300979820007985
3	29.983106551586445	25.329360813342756	30.972386890684035	13.715145744386767
4	27.119329176521177	26.919187885861717	30.57480726111128	15.38667567650582
5	23.28113769696225	31.857480726111127	29.848450410050063	15.012931166876555
6	20.455324507786344	41.14642012470436	28.03501551125718	10.363239856252111
7	87.32905365973524	3.742973861228	7.267868661117424	1.6601038179193415
8	85.48588629173449	5.871671222778511	6.821390177227632	1.8210523082593606
9	84.22532788647604	4.23405104892957	8.597966643118223	2.9426544214761803
10	50.28448567128421	23.177688361949812	14.202168504469084	12.335657462296895
11	42.06186073655435	22.41238443345517	20.589857787879716	14.93589704211076
12	36.819854409190036	21.602358939705745	25.827318241852748	15.750468409251466
13	23.26184845041005	33.35577602358939	26.547777743649597	16.834597782350954
14	17.771047700955247	35.48484196946893	29.8522591147833	16.891851214792517
15	16.620450287188625	22.954326258561906	42.825444604846886	17.599778849402586
16	19.819639401664773	19.58792272015235	40.078385600638875	20.514052277544
17	19.990908253217434	19.668151242436345	28.81801148754492	31.522929016801303
18	23.281874865620296	23.111588905611697	33.04788524741223	20.558650981355775
19	28.492182940688636	25.003777989372484	25.92302730595571	20.581011763983167
20	30.411155819025094	24.064010811806984	24.968885339558312	20.55594802960961
21	24.84368952913352	27.533617962342966	27.843965967380285	19.778726541143225
22	23.774672113523973	22.46251190220229	27.78536105906564	25.9774549252081
23	21.873022698651596	27.881315846054612	26.68341677673004	23.562244678563747
24	21.80262309180821	24.4402125502964	34.848788279018336	18.908376078877044
25	21.235248947998894	24.471910802592376	32.40273981017907	21.89010043922966
26	19.04770095524772	30.84608532727217	27.226095770494823	22.880117946985287
27	19.94458948920355	29.454188039438524	30.035936972079735	20.56528549927819
28	18.279694075006912	26.663636084405812	35.75857726448997	19.298092576097307
29	19.3429370027951	25.59584728322634	33.94305372116595	21.118161992812606
30	22.227232238842646	27.432748717633686	31.291212335288876	19.04880670823479
31	30.2939460023958	24.304204932886936	25.217556900205796	20.184292164511472
32	29.350247258654054	25.241023435820253	25.930030408207145	19.47869889731855
33	27.251036643425376	25.578769542648278	25.497680990263234	21.672512823663116
34	20.517983843720245	26.609699910925457	28.060079245630742	24.81223699972356
35	21.40148048038824	25.24950087538778	30.206960100746382	23.142058543477592
36	29.441901895137757	24.645268298676168	25.816137850539057	20.09669195564702
37	21.748195472555825	30.393586632675003	26.405749915532756	21.452467979236417
38	22.172313173818225	31.125103664342536	23.452283687071905	23.25029947476733
39	22.113216819731548	28.490708603372543	25.449273581718217	23.94680099517769
40	24.246828639002366	24.47854532051479	26.799643701815278	24.474982338667566
41	19.94360659765949	23.83856006388795	27.648616272998126	28.56921706545443
42	22.4851184077157	25.48785207482262	24.790736247197223	27.23629327026446
43	21.6304942101545	24.984980188592314	26.3518137420524	27.032711859200788
44	20.79773934944866	27.275608932026906	28.850201185612924	23.076450532911508
45	20.328285775716438	31.695672205670057	24.695641490309303	23.280400528304206
46	23.274134594710816	28.385539208158	27.02939460023958	21.310931596891606
47	22.38990078938477	26.612280001228616	27.184322879872227	23.81349632951439
48	22.28657431581534	25.289553705808277	30.15548115612618	22.268390822250208
49	22.424179131983905	24.35531529317812	30.694720029486746	22.52578554535123
50	20.58408330005836	29.30872009091747	27.633135731179166	22.47406087784501
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	414.0
1	810.5
2	1207.0
3	4341.5
4	7476.0
5	5532.0
6	3588.0
7	3682.5
8	3777.0
9	3934.0
10	4091.0
11	4027.5
12	3964.0
13	3933.0
14	3902.0
15	3750.0
16	3598.0
17	3375.0
18	3152.0
19	3081.5
20	3011.0
21	3298.5
22	3586.0
23	3647.0
24	3708.0
25	4357.0
26	5006.0
27	5645.0
28	6284.0
29	7722.5
30	9161.0
31	10795.0
32	12429.0
33	14789.0
34	17149.0
35	18351.5
36	19554.0
37	21457.0
38	23360.0
39	28636.0
40	33912.0
41	43442.5
42	52973.0
43	60374.0
44	67775.0
45	68114.0
46	68453.0
47	65869.5
48	63286.0
49	63242.5
50	63199.0
51	59258.0
52	55317.0
53	50151.0
54	44985.0
55	43499.0
56	42013.0
57	41075.0
58	40137.0
59	39303.0
60	38469.0
61	35225.0
62	31981.0
63	28230.5
64	24480.0
65	20915.0
66	17350.0
67	14424.0
68	11498.0
69	9757.0
70	8016.0
71	6529.0
72	5042.0
73	4230.5
74	3419.0
75	2532.0
76	1645.0
77	1257.5
78	870.0
79	635.5
80	401.0
81	284.0
82	167.0
83	113.0
84	59.0
85	47.0
86	35.0
87	24.5
88	14.0
89	9.5
90	5.0
91	5.0
92	5.0
93	3.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	813925.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.368494003488784
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.57012944380912	23.253195168606773
2	6.415889785925587	3.8968182098185227
3	2.419270674762299	2.2040882093800542
4	1.4972435150844063	1.8187612283841308
5	1.1428040800037012	1.7352619425377456
6	0.9491380110966489	1.7294335199083108
7	0.8125454111345692	1.7273046311921743
8	0.7342487741033599	1.7838423594741502
9	0.696147439165288	1.9026854398651607
>10	8.52696984803862	52.93057869209764
>50	0.1918335715828753	3.7043937103282425
>100	0.040197490678625394	2.115380537675154
>500	0.003183959657712901	0.6959411002133205
>1k	3.9799495721411265E-4	0.5023152505186801
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4156	0.5106121571397856	No Hit
GTACAAGGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACT	857	0.10529225665755444	No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	837	0.10283502779740149	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0229750898424302	0.0
2	0.0	0.0	0.0	0.0911631907116749	0.0
3	0.0	0.0	0.0	0.13871056915563473	0.0
4	0.0	0.0	0.0	0.22459071781798076	0.0
5	0.0	0.0	0.0	0.435052369690082	0.0
6	0.0	0.0	0.0	0.6273305279970514	0.0
7	0.0	0.0	0.0	0.7603894707743343	0.0
8	0.0	0.0	0.0	1.0681573855084927	0.0
9	0.0	0.0	0.0	1.188561599655988	0.0
10	0.0	0.0	0.0	1.4119237030438923	0.0
11	0.0	0.0	0.0	1.6188223730687716	0.0
12	0.0	0.0	0.0	1.7972171883158767	0.0
13	0.0	0.0	0.0	1.8682311023742975	0.0
14	0.0	0.0	0.0	1.8988236016832019	0.0
15	0.0	0.0	0.0	1.9420708296218938	0.0
16	0.0	0.0	0.0	2.044045827318242	0.0
17	0.0	0.0	0.0	2.161255643947538	0.0
18	0.0	0.0	0.0	2.347759314433148	0.0
19	0.0	0.0	0.0	2.4267592222870658	0.0
20	0.0	0.0	0.0	2.5079706361151213	0.0
21	0.0	0.0	0.0	2.609208465153423	0.0
22	0.0	0.0	0.0	2.7070061737875113	0.0
23	0.0	0.0	0.0	2.8213901772276317	0.0
24	0.0	0.0	0.0	2.915870626900513	0.0
25	0.0	0.0	0.0	2.9965905949565377	0.0
26	0.0	0.0	0.0	3.0793992075436925	0.0
27	0.0	0.0	0.0	3.16417360321897	0.0
28	0.0	0.0	0.0	3.254845348158614	0.0
29	0.0	0.0	0.0	3.356451761525939	0.0
30	0.0	0.0	0.0	3.4984795896427805	0.0
31	0.0	0.0	0.0	3.6150750990570386	0.0
32	0.0	0.0	0.0	3.721350247258654	0.0
33	0.0	0.0	0.0	3.8426144915072027	0.0
34	0.0	0.0	0.0	3.965844518843874	0.0
35	0.0	0.0	0.0	4.123475750222687	0.0
36	0.0	0.0	0.0	4.240071259636944	0.0
37	0.0	0.0	0.0	4.37927327456461	0.0
38	0.0	0.0	0.0	4.526215560401757	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTTACG	40	8.307325E-9	44.0	1
GCGTAGT	30	2.5268018E-6	44.0	16
GTACGTC	20	7.855126E-4	44.0	28
CGTTTTT	3250	0.0	42.17231	1
CGGGTAT	75	0.0	41.066666	6
CGACGGT	130	0.0	40.615383	28
CGGTCTA	130	0.0	40.615383	31
TATAGCG	60	3.6379788E-12	40.333332	1
CGACCCG	585	0.0	40.239315	32
ACCCGCT	595	0.0	39.563026	34
CCGCTGA	605	0.0	39.272728	36
GCGCGAC	630	0.0	39.11111	9
TTACGGG	180	0.0	39.11111	3
CGCTGAA	620	0.0	39.032257	37
CACGACG	130	0.0	38.923077	26
TCAGACG	635	0.0	38.803146	22
AGACGTG	620	0.0	38.67742	24
ATAGGGC	370	0.0	38.64865	4
ACGTAAG	40	4.1225576E-7	38.5	1
AGGGCGC	360	0.0	38.5	6
>>END_MODULE