FastQCFastQC Report
Thu 26 May 2016
SRR1546552_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546552_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2383988
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66230.27781180106611275No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACGATCTGTCGTATGCC39650.16631795126485538No Hit
GAATGATACGGCGACCACCGATGAATGATACGGCGACCACCTGTCGAATG33330.13980775071015458No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACGATCTGTCGTATG24390.10230756195081518No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGTTC254.4442837E-544.030
CCGATGA65000.042.81538418
CGATGAA65950.042.66565719
CCCCGTA1600.042.62540
TATAGCG1200.042.166671
CGATCGC800.041.2534
CGTTTTT61900.041.2277871
ACCGATG68100.040.8340717
CCACCGA74000.040.28378315
GATGAAT70550.040.2579720
CGTTAGG3450.040.1739122
TGAATGA69600.040.14367722
GTCGAAT6500.039.9384643
TTTAGCG1600.039.8751
GTATCGA501.3496901E-939.60000210
CACCGAT73050.039.5728916
GCGAACC3400.039.4705933
ATGAATG71450.039.22743221
TAGAACG900.039.111111
ACCACCG78050.038.67264614