##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546552_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2383988
Sequences flagged as poor quality	0
Sequence length	50
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.62151613179261	31.0	30.0	31.0	28.0	33.0
2	30.946981696216593	31.0	31.0	33.0	28.0	34.0
3	31.12382864343277	31.0	31.0	34.0	28.0	34.0
4	34.95147123223774	35.0	35.0	37.0	32.0	37.0
5	34.71598766436744	35.0	35.0	37.0	32.0	37.0
6	34.769425013884295	35.0	35.0	37.0	32.0	37.0
7	35.40157542739309	35.0	35.0	37.0	35.0	37.0
8	34.37686179628421	35.0	35.0	37.0	33.0	37.0
9	36.543933945976235	39.0	37.0	39.0	32.0	39.0
10	35.87986390871095	38.0	35.0	39.0	30.0	39.0
11	35.56889422262193	37.0	35.0	39.0	30.0	39.0
12	34.894700392787215	37.0	33.0	39.0	30.0	39.0
13	34.390891648783466	37.0	33.0	39.0	26.0	39.0
14	35.3003475688636	37.0	33.0	40.0	26.0	41.0
15	35.74706416307465	37.0	33.0	40.0	29.0	41.0
16	35.95071787274097	37.0	34.0	40.0	30.0	41.0
17	35.7828197960728	37.0	34.0	40.0	29.0	41.0
18	35.81699740099363	37.0	34.0	40.0	30.0	41.0
19	35.820507905241136	37.0	34.0	40.0	29.0	41.0
20	35.437532403686596	37.0	34.0	40.0	29.0	41.0
21	35.331040676379246	37.0	34.0	40.0	29.0	41.0
22	35.218360159531	37.0	33.0	40.0	27.0	41.0
23	35.00079195029505	36.0	33.0	40.0	27.0	41.0
24	35.10788519069727	36.0	33.0	40.0	27.0	41.0
25	35.08687417889687	36.0	33.0	40.0	27.0	41.0
26	34.77733738592644	36.0	33.0	40.0	27.0	41.0
27	34.47968404203377	36.0	33.0	40.0	25.0	41.0
28	34.587482822900114	36.0	33.0	40.0	25.0	41.0
29	34.39009214811484	36.0	33.0	40.0	25.0	41.0
30	34.57425163213909	36.0	33.0	40.0	25.0	41.0
31	34.54895494440408	36.0	33.0	40.0	25.0	41.0
32	34.147279684293714	36.0	33.0	40.0	24.0	41.0
33	33.893157180321374	36.0	32.0	40.0	23.0	41.0
34	33.81754899772985	36.0	32.0	40.0	23.0	41.0
35	33.553914281447724	36.0	32.0	40.0	23.0	41.0
36	33.551579957617236	36.0	32.0	40.0	21.0	41.0
37	33.559920184162	36.0	32.0	40.0	22.0	41.0
38	33.567976852232476	35.0	32.0	40.0	22.0	41.0
39	33.52975853905305	36.0	32.0	40.0	22.0	41.0
40	33.45448928434204	35.0	32.0	40.0	21.0	41.0
41	33.60942504744151	36.0	32.0	40.0	22.0	41.0
42	33.35178826403489	35.0	32.0	40.0	21.0	41.0
43	33.39399233553189	35.0	32.0	40.0	22.0	41.0
44	33.231819539360096	35.0	31.0	39.0	21.0	41.0
45	33.226996528506014	35.0	31.0	39.0	22.0	41.0
46	33.19344057100959	35.0	31.0	39.0	22.0	41.0
47	33.01534613429262	35.0	31.0	39.0	20.0	41.0
48	33.163509631759894	35.0	31.0	39.0	21.0	41.0
49	33.22799946979599	35.0	31.0	39.0	22.0	41.0
50	32.55343189646928	35.0	31.0	39.0	19.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	1.0
12	4.0
13	11.0
14	32.0
15	88.0
16	249.0
17	717.0
18	1618.0
19	3496.0
20	7059.0
21	12569.0
22	20415.0
23	29418.0
24	36714.0
25	44451.0
26	52178.0
27	59792.0
28	66522.0
29	75746.0
30	89511.0
31	109525.0
32	132905.0
33	165469.0
34	236933.0
35	261467.0
36	190164.0
37	225928.0
38	276734.0
39	284271.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.58839725703317	23.144369854210677	28.3761495443769	11.89108334437925
2	29.961811888314877	28.145275899039763	29.20102785752277	12.691884355122593
3	28.317759988724777	28.149009139307747	30.263868777862974	13.269362094104501
4	25.26946444361297	26.51577944184283	33.86434831047807	14.350407804066128
5	25.536873507752556	31.873398691604155	29.010464817775926	13.579262982867363
6	19.463604682573905	44.68982226420603	26.286290031661235	9.560283021558833
7	86.80303759918255	3.3102515616689345	8.743877905425698	1.142832933722821
8	85.94019768555881	8.391275459440232	4.606650704617641	1.0618761503833074
9	85.02060413055771	3.5965785062676487	5.835809576222699	5.547007786951948
10	49.60352149423571	25.245051569051526	13.097297469618136	12.054129467094633
11	42.438720329129175	23.859474124869756	20.96927501312926	12.732530532871811
12	35.90324280155773	22.608293330335556	23.339043652904294	18.149420215202426
13	23.899071639622345	35.26083184982475	24.486616543371863	16.35347996718104
14	15.871682240011275	40.24214886987686	27.56419075934946	16.321978130762403
15	15.737075857764385	23.803139948691015	42.17240187450608	18.28738231903852
16	17.680458123111357	21.351030290420926	40.04739117814351	20.921120408324203
17	18.62404508747527	24.233930707704904	27.36838440461949	29.773639800200336
18	21.20455304305223	24.461448631452843	32.05049689847432	22.283501427020607
19	27.644518344891	25.188214034634406	25.05025193079831	22.11701568967629
20	32.21891217573243	24.745258784859654	24.8904776366324	18.145351402775518
21	23.001667793629835	30.684508479069528	27.39506239125365	18.918761336046995
22	22.115505614961148	24.08820849769378	29.37351194720779	24.422773940137283
23	22.67909905586773	29.25543249378772	26.647449567699166	21.41801888264538
24	19.610459448621388	26.612927581850244	35.50466696980018	18.271945999728185
25	18.005166133386577	28.814868195645282	33.167994134198665	20.01197153676948
26	18.108270679214826	32.781373060602654	29.64566096809212	19.464695292090397
27	21.251575091820932	31.880865172140126	28.431812576237796	18.435747159801142
28	16.62923638877377	31.232581707626046	35.21431315929443	16.923868744305757
29	17.825257509685454	25.522360011879254	37.049053938190966	19.60332854024433
30	19.923841898533045	31.549529611726236	29.51822744074215	19.008401048998568
31	27.228199135230547	25.27395272123853	25.32944796701997	22.168400176510954
32	30.851833146811142	26.130710389481827	26.421903130384884	16.595553333322147
33	29.16633808559439	26.1502994142588	25.35377694854169	19.329585551605124
34	20.15714844202236	26.97584887172251	28.058111030760223	24.80889165549491
35	22.14600073490303	25.46581610310119	30.834509234106882	21.5536739278889
36	27.943932603687603	27.457394919773087	25.784987172754224	18.813685303785086
37	20.89658169420316	31.481953768223665	27.49774747188325	20.123717065689927
38	20.939786609664143	31.682625919257983	24.508806252380467	22.86878121869741
39	21.072421505477376	31.710562301488093	25.841321348932965	21.375694844101563
40	23.39919496239075	25.59400466780873	26.07341144334619	24.933388926454327
41	18.59375970013272	25.083138002372497	26.767122988874103	29.555979308620685
42	22.09558101802526	26.114728765413247	25.478442005580565	26.311248210980924
43	22.333837250858647	27.424215222559845	25.272316806963797	24.969630719617715
44	20.296117262335216	29.483663508373365	28.848215678937983	21.37200355035344
45	19.48264840259263	33.663969785082806	24.550207467487255	22.303174344837306
46	22.58589388872763	31.991226465905033	25.912672379223384	19.510207266143954
47	21.060886212514493	28.114403260419095	27.369349174576378	23.45536135249003
48	22.310976397532205	25.359691407842654	30.093020602452697	22.236311592172445
49	21.22145749055784	24.883430621295073	31.864254350273573	22.030857537873512
50	20.384792205329894	31.68912762983706	26.762047459970432	21.16403270486261
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	484.0
1	1421.5
2	2359.0
3	7859.5
4	13360.0
5	10422.5
6	7485.0
7	7611.0
8	7737.0
9	8079.0
10	8421.0
11	8388.5
12	8356.0
13	8288.0
14	8220.0
15	7683.0
16	7146.0
17	6964.0
18	6782.0
19	7211.0
20	7640.0
21	8707.0
22	9774.0
23	11075.5
24	12377.0
25	14320.5
26	16264.0
27	20464.5
28	24665.0
29	30755.5
30	36846.0
31	41881.5
32	46917.0
33	53855.5
34	60794.0
35	67102.5
36	73411.0
37	82816.0
38	92221.0
39	110975.5
40	129730.0
41	159861.0
42	189992.0
43	209848.0
44	229704.0
45	227680.0
46	225656.0
47	211914.0
48	198172.0
49	191273.5
50	184375.0
51	173963.0
52	163551.0
53	156337.5
54	149124.0
55	135973.0
56	122822.0
57	109390.0
58	95958.0
59	87404.5
60	78851.0
61	71991.0
62	65131.0
63	54265.5
64	43400.0
65	33352.0
66	23304.0
67	18093.5
68	12883.0
69	10533.5
70	8184.0
71	6820.0
72	5456.0
73	4365.5
74	3275.0
75	2394.5
76	1514.0
77	1137.5
78	761.0
79	602.5
80	444.0
81	352.5
82	261.0
83	188.0
84	115.0
85	71.5
86	28.0
87	25.5
88	23.0
89	17.0
90	11.0
91	7.0
92	3.0
93	2.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2383988.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.719914014411227
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.87843767551472	16.550743602421708
2	6.453586770245791	2.6743552592806927
3	2.358253340057403	1.465884192905624
4	1.2699863928363997	1.052560354361699
5	0.8514256437352933	0.8820733063930001
6	0.5975422598498443	0.7428614546439445
7	0.44429308056115935	0.6444000098497571
8	0.361834479753691	0.5997743446356557
9	0.32255671787985735	0.6015012713317209
>10	4.760526187601853	25.060499958831567
>50	2.032980519690495	29.80314447727011
>100	0.6640747340665296	18.578386639893903
>500	0.0028650352226330814	0.382469154871555
>1k	0.0014325176113165407	0.6805152401921936
>5k	2.0464537304522008E-4	0.28083073311688567
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6623	0.27781180106611275	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACGATCTGTCGTATGCC	3965	0.16631795126485538	No Hit
GAATGATACGGCGACCACCGATGAATGATACGGCGACCACCTGTCGAATG	3333	0.13980775071015458	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACGATCTGTCGTATG	2439	0.10230756195081518	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.007676213135301016	0.0
2	0.0	0.0	0.0	0.04110758946773222	0.0
3	0.0	0.0	0.0	0.06094829336389277	0.0
4	0.0	0.0	0.0	0.11824723949952769	0.0
5	0.0	0.0	0.0	0.27730844282773237	0.0
6	0.0	0.0	0.0	0.37131059384527104	0.0
7	0.0	0.0	0.0	0.4543227566581711	0.0
8	0.0	0.0	0.0	0.6342733268791622	0.0
9	0.0	0.0	0.0	0.7067149666860739	0.0
10	0.0	0.0	0.0	0.9873791311030089	0.0
11	0.0	0.0	0.0	1.0752151437003878	0.0
12	0.0	0.0	0.0	1.2737060757017233	0.0
13	0.0	0.0	0.0	1.3183371728381184	0.0
14	0.0	0.0	0.0	1.3381359302144138	0.0
15	0.0	0.0	0.0	1.3818442039137782	0.0
16	0.0	0.0	0.0	1.444428411552407	0.0
17	0.0	0.0	0.0	1.5085226939061773	0.0
18	0.0	0.0	0.0	1.606132245632109	0.0
19	0.0	0.0	0.0	1.7143542668838938	0.0
20	0.0	0.0	0.0	1.7711918013010133	0.0
21	0.0	0.0	0.0	1.8386837517638512	0.0
22	0.0	0.0	0.0	1.913809968842125	0.0
23	0.0	0.0	0.0	1.991494923632166	0.0
24	0.0	0.0	0.0	2.049255281486316	0.0
25	4.194651986503288E-5	0.0	0.0	2.1042471690293745	0.0
26	4.194651986503288E-5	0.0	0.0	2.160203826529328	0.0
27	4.194651986503288E-5	0.0	0.0	2.2215296385720062	0.0
28	4.194651986503288E-5	0.0	0.0	2.2780735473500706	0.0
29	4.194651986503288E-5	0.0	0.0	2.3458591234519637	0.0
30	4.194651986503288E-5	0.0	0.0	2.4310944518177107	0.0
31	4.194651986503288E-5	0.0	0.0	2.5123448607962793	0.0
32	4.194651986503288E-5	0.0	0.0	2.589148938669154	0.0
33	4.194651986503288E-5	0.0	0.0	2.6644429418268882	0.0
34	4.194651986503288E-5	0.0	0.0	2.7399886241038125	0.0
35	4.194651986503288E-5	0.0	0.0	2.840240806581241	0.0
36	4.194651986503288E-5	0.0	0.0	2.9234207554736016	0.0
37	4.194651986503288E-5	0.0	0.0	3.0077752069221826	0.0
38	4.194651986503288E-5	0.0	0.0	3.101903197499316	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCGTTC	25	4.4442837E-5	44.0	30
CCGATGA	6500	0.0	42.815384	18
CGATGAA	6595	0.0	42.665657	19
CCCCGTA	160	0.0	42.625	40
TATAGCG	120	0.0	42.16667	1
CGATCGC	80	0.0	41.25	34
CGTTTTT	6190	0.0	41.227787	1
ACCGATG	6810	0.0	40.83407	17
CCACCGA	7400	0.0	40.283783	15
GATGAAT	7055	0.0	40.25797	20
CGTTAGG	345	0.0	40.173912	2
TGAATGA	6960	0.0	40.143677	22
GTCGAAT	650	0.0	39.93846	43
TTTAGCG	160	0.0	39.875	1
GTATCGA	50	1.3496901E-9	39.600002	10
CACCGAT	7305	0.0	39.57289	16
GCGAACC	340	0.0	39.47059	33
ATGAATG	7145	0.0	39.227432	21
TAGAACG	90	0.0	39.11111	1
ACCACCG	7805	0.0	38.672646	14
>>END_MODULE